Muscle fibre types in the myotome of stickleback, <i>Gasterosteus aculeatus</i> L.; a histochemical, immunohistochemical and ultrastructural study

Kronnie, G. te; Tatarczuch, Liliana; Raamsdonk, W. van; Kilarski, Wincenty

doi:10.1111/j.1095-8649.1983.tb04754.x

Cited by 50 publications

(29 citation statements)

References 20 publications

(49 reference statements)

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“…While there was no evidence of EPOC after swimming at 1.9L F s -1 (Fig.1), it remains unclear how E. lateralis powered swimming at this speed. The red oxidative component in the myotomal musculature is usually limited in labriform swimmers (Kronnie et al, 1983). In E. lateralis, only about 0.3% of the axial muscles is red (Cannas et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

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Partition of aerobic and anaerobic swimming costs related to gait transitions in a labriform swimmer

Svendsen

Tudorache

Jordan

et al. 2010

Journal of Experimental Biology

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SUMMARYMembers of the family Embiotocidae exhibit a distinct gait transition from exclusively pectoral fin oscillation to combined pectoral and caudal fin propulsion with increasing swimming speed. The pectoral-caudal gait transition occurs at a threshold speed termed U p-c . The objective of this study was to partition aerobic and anaerobic swimming costs at speeds below and above the U p-c in the striped surfperch Embiotoca lateralis using swimming respirometry and video analysis to test the hypothesis that the gait transition marks the switch from aerobic to anaerobic power output. Exercise oxygen consumption rate was measured at 1.4, 1.9 and 2.3Ls , E. lateralis switched to an unsteady burst and flap gait. This swimming speed resulted in EPOC, suggesting that anaerobic metabolism constituted 25% of the total costs. Burst activity correlated positively with the magnitude of the EPOC. Collectively, these data indicate that steady axial propulsion does not lead to EPOC whereas transition to burst-assisted swimming above U p-c is associated with anaerobic metabolism in this labriform swimmer.

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Section: Discussionmentioning

confidence: 99%

“…The myotomal musculature is usually spatially divided into separate regions of red and white fibres (Sänger and Stoiber, 2001). In labriform swimmers, and other median and paired fin swimmers, the red myotomal musculature may be scarce (Fernández et al, 1999;Martinez et al, 2000) or absent (Davison, 1987;Kronnie et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

Partition of aerobic and anaerobic swimming costs related to gait transitions in a labriform swimmer

Svendsen

Tudorache

Jordan

et al. 2010

Journal of Experimental Biology

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“…The mammalian literature suggests that white fibres should have the highest activities of PK and LDH, but in carp (Cyprinus carpio), pink fibres have LDH activities that are higher (Johnston et al, 1977) or similar to those of white fibres (Jabarsyah et al, 2000). Te Kronnie et al (Te Kronnie et al, 1983) found that the axial musculature of threespine stickleback is primarily composed of white fibres, with some pink fibres and no red fibres. To date, the fibre-type composition of pectoral abductors and adductors had not been separately studied due to their small size and similar 'red' phenotype (e.g.…”

Section: Muscle Histologymentioning

confidence: 99%

Mechanisms underlying parallel reductions in aerobic capacity in non-migratory threespine stickleback (Gasterosteus aculeatus) populations

Dalziel

Schulte

2012

Journal of Experimental Biology

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SUMMARYNon-migratory, stream-resident populations of threespine stickleback, Gasterosteus aculeatus, have a lower maximum oxygen consumption (M O2,max ) than ancestral migratory marine populations. Here, we examined laboratory-bred stream-resident and marine crosses from two locations (West and Bonsall Creeks) to determine which steps in the oxygen transport and utilization cascade evolved in conjunction with, and thus have the potential to contribute to, these differences in M O2,max . We found that West Creek stream-resident fish have larger muscle fibres (not measured in Bonsall fish), Bonsall Creek stream-resident fish have smaller ventricles, and both stream-resident populations have evolved smaller pectoral adductor and abductor muscles. However, many steps of the oxygen cascade did not evolve in stream-resident populations (gill surface area, hematocrit, mean cellular hemoglobin content and the activities of mitochondrial enzymes per gram ventricle and pectoral muscle), arguing against symmorphosis. We also studied F1 hybrids to determine which traits in the oxygen cascade have a genetic architecture similar to that of M O2,max . In West Creek, M O2,max , abductor and adductor size all showed dominance of marine alleles, whereas in Bonsall Creek, M O2,max and ventricle mass showed dominance of stream-resident alleles. We also found genetically based differences among marine populations in hematocrit, ventricle mass, pectoral muscle mass and pectoral muscle pyruvate kinase activity. Overall, reductions in pectoral muscle mass evolved in conjunction with reductions in M O2,max in both stream-resident populations, but the specific steps in the oxygen cascade that have a genetic basis similar to that of M O2,max , and are thus predicted to have the largest impact on M O2,max , differ among populations. Supplementary material available online at

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“…In agreement with Mosse (1979), we noticed that the ATPase activity of fish muscle fibres was more sensitive to pH treatment than those of other vertebrates. White fibres, however, showed positive reactions for ATPase but only after short periods of alkaline preincubation (Te Kronnie et al 1983). The most stable were intermediate fibres, which displayed stability after alkaline as well as acid pre-treatment.…”

Section: Ultrastructurementioning

confidence: 99%

Effect of Propolis on the Fish Muscular Development and Histomorphometrical Characteristics

Velotto¹,

Vitale²,

Varricchio

et al. 2010

Acta Vet. Brno

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The aim of our study was to evaluate the effect of propolis on mortality of fish eggs due to mycosis, to study its efficacy on the fish growth rate and to analyze the histochemical and ultrastructural characteristics of muscle fibres. We evaluated the muscular development of fish in two different experimental conditions: traditional feeding and feeding with addition of propolis (groups A and B, respectively). The study was carried out on two species of teleosts bred in Italy, Oncorhynchus mykiss (rainbow trout) and Salmo trutta (brown trout). The use of propolis reduced mortality of fish eggs. The muscles were stained for myosin ATPase and succinic dehydrogenase to evaluate fibre type. The area, maximum and minimum diameter for each fibre type in relation to the body length were measured. The animals fed propolis showed a more rapid muscular growth compared to control fed the standard diet. Fibres were larger in the Salmo trutta than in the Oncorhynchus mykiss in both groups. In fish of A group, myotomal muscle, the presence of giant fibres and index of environmental stress was noticed. This type of study is valuable in order to extend the use of propolis as an antifungal agent and a natural product that can improve fish farming. ATPase and SDH activities, specific growth-rate, ultrastructural analysisEuropean aquaculture has shown a steady increase during the last twenty years, thanks to many initiatives promoted by the European Community to support this sector. But there are still many problems to solve, such as the support of ecocompatible activities, the improvement of animal health and welfare, product quality and consumer health assurance. Propolis is a mixture of various amounts of beeswax and resins collected by the honeybee from plants, particularly from flowers and leaf buds (Krell 1996). The colour of propolis ranges from yellow to dark brown depending on the origin of the resins. Propolis has attracted the researchers' interest in the last decades due to its biological and pharmacological properties, such as immunomodulatory, antitumor, antimicrobial, antiinflammatory, and antioxidant properties (Kanbur et al. 2009). The goal of this study was to analyze, for the first time, the influence of propolis on the fish muscular development and on the structural and ultrastructural characteristics of muscle fibre types. This study opens a new perspective on the investigation of biological properties of propolis, mainly with respect to the influence on the development of the trout and the mortality of fish eggs. Materials and MethodsThe following species of freshwater fish were used in our study: Salmo trutta (brown trout) and Oncorhynchus mykiss (rainbow trout). Experiment with eggsIn order to evaluate the effect of propolis on mortality of fish eggs, approximately 600 eggs (half and half) were placed in 3 "Californian tanks" arranged in cascade. Untreated samples (about 250 eggs) were used as control. The eggs were rinsed in propolis solution (1%) for 15 min, once every four days and then they were ...

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Muscle fibre types in the myotome of stickleback, Gasterosteus aculeatus L.; a histochemical, immunohistochemical and ultrastructural study

Cited by 50 publications

References 20 publications

Partition of aerobic and anaerobic swimming costs related to gait transitions in a labriform swimmer

Partition of aerobic and anaerobic swimming costs related to gait transitions in a labriform swimmer

Mechanisms underlying parallel reductions in aerobic capacity in non-migratory threespine stickleback (Gasterosteus aculeatus) populations

Effect of Propolis on the Fish Muscular Development and Histomorphometrical Characteristics

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