Muscle fatty acid profiles of sea lamprey (Petromyzon marinus L.) indicate the use of fast metabolized energy during ontogenesis

Martins, Elói; Almeida, Pedro R.; Quintella, Bernardo R.; Silva, Marco Gomes da; Lança, Maria João

doi:10.1007/s10695-018-0580-3

Cited by 5 publications

(3 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Larval lampreys are known to have unusual lipid origins, relying on muscle instead of the liver cells for synthesis [31]. Alternatively, larval lampreys may retain lipid scaffolding from other organisms they feed on, such as bacteria and algae [41], and then saturate any double bonds that occur primarily with 1 H. Stream algae δ 13 C is variable and dependent on water velocity [42] and their lipids may be relatively enriched in δ 13 C compared to larval lamprey muscle.…”

Section: Discussionmentioning

confidence: 99%

Are lipids always depleted? Comparison of hydrogen, carbon, and nitrogen isotopic values in the muscle and lipid of larval lampreys

Evans,

Beharie

2024

PLoS ONE

View full text Add to dashboard Cite

Stable isotope ratios in organisms can be used to estimate dietary source contributions, but lipids must first be accounted for to interpret values meaningfully. Lipids are depleted in heavy isotopes because during lipid synthesis light isotopes of carbon (12C) and hydrogen (1H) are preferentially incorporated. Prior work in larval lampreys has noted unusual lipid effects, which suggest lipids are enriched in the heavy isotope of carbon (13C), but still depleted in the heavy isotope of hydrogen (deuterium; 2H); nitrogen, a relatively rare element in lipids, has not been identified as being as sensitive to lipid content. Our objective was to determine if stable isotope ratios of hydrogen, carbon, and nitrogen behaved as expected in larval lampreys, or if their lipids presented different isotopic behavior. The δ2H, δ13C, and δ15N were measured from the muscle of four lamprey species before and after lipid extraction. In addition, muscle of least brook lamprey (Lampetra aepyptera) was collected every three months for a year from two streams in Maryland. Isotopic ratios were measured in bulk and lipid-extracted muscles, as well as in extracted lipids. The difference between muscle samples before and after lipid extraction (Δδ2H, Δδ13C, Δδ15N) was positively related to lipid proxy (%H or C:N ratio) and were fit best by linear models for Δδ2H and Δδ15N, and by a non-linear model for Δδ13C. The difference between lipid-extracted muscle and lipid δ13C (ΔMLδ13C) was negative and varied between months (ANOVA, F3,53 = 5.05, p < 0.005). Our work suggests that while lipids are often depleted in 13C, this is not a universal rule; however, the depletion of 2H in lipid synthesis appears broadly true.

show abstract

Section: Discussionmentioning

confidence: 99%

Are lipids always depleted? Comparison of hydrogen, carbon, and nitrogen isotopic values in the muscle and lipid of larval lampreys

Evans,

Beharie

2024

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Larval lampreys are known to have unusual lipid origins, relying on muscle instead of the liver cells for synthesis 20 . Larval lampreys may retain lipid scaffolding from other organisms they feed on, such as bacteria and algae 37 , and then saturate any double bonds that occur primarily with 1 H. Stream algae δ 13 C is variable and dependent on water velocity 38 and their lipids may be relatively enriched in δ 13 C compared to larval lamprey muscle.…”

Section: Discussionmentioning

confidence: 99%

Are lipids always light? Lipids in larval lampreys are enriched in 13C but depleted in 2H relative to muscle

Evans

Beharie

2022

Preprint

View full text Add to dashboard Cite

Stable isotope ratios in organisms can be used to estimate source contributions to the organism. However, during lipid synthesis light isotopes of carbon (12C) and hydrogen (1H) are preferentially incorporated into the lipids, potentially causing source contributions to be poorly estimated. Contrary to expectations and other published examples in animals, larval lampreys, which are basal vertebrates, have lipids which are enriched in heavy isotopes of carbon (13C), but still depleted in heavy hydrogen (deuterium; 2H). Four lamprey species were collected and their isotopes ratios of δ2H, δ13C, δ15N were measured in their muscle before and after lipid extraction. Larval lamprey of one species was collected every three months for a year from two streams in Maryland and the isotope ratios of muscle before and after lipid extraction, as well as the extracted lipid were measured. Muscle δ13C was positively related to C:N ratios in samples when lipids were not removed and δ2H was negatively associated with the percent hydrogen in a sample. As expected, the measured difference between muscle and lipid δ2H (ΔMLδ2H) was the same for all months and was 111‰ (SE = ± 21, n = 35), but the ΔMLδ13C was different between months (ANOVA, F3,53 = 5.05, p < 0.005) and was always negative. Our work suggests that while lipids are often enriched in 12C relative to muscle, this is not a universal rule. The physiological mechanism(s) for generating heavy carbon-backbones in lipids remains unknown and requires exploration.

show abstract

“…In the sea lamprey, gonad development occurs when they stop feeding and initiate the atrophy of the digestive tract (Beamish et al, 1979). Consequently, gonadal growth and the energy expenditure for migration depend on mobilization of lipid and protein reserves accumulated during the parasitic phase (Hardisty 2006) and are not restored during the process of reproductive migration after feeding has ceased (Martins et al, 2019). A drawback of this study is that we only measured more polar molecules due to the extraction and optimized analytical methods.…”

Section: Discussionmentioning

confidence: 99%

Plasma metabolomic profiles reveal sex- and maturation-dependent metabolic strategies in sea lamprey (Petromyzon marinus)

et al. 2022

View full text Add to dashboard Cite

Introduction Adult sea lamprey (Petromyzon marinus) cease feeding and migrate to spawning streams where males build nests, undergo nal sexual maturation, and subsequently produce and release large quantities of bile acid pheromones that attract mature females. These animals are predicted to rearrange their metabolic pathways drastically to support their reproductive strategies, presenting advantageous opportunities to examine how sex and the maturation processes affect metabolism.Objectives The objective is to investigate the metabolic differences between sexes and maturation states in sea lamprey that support changes in physiological functions.Methods We compared plasma metabolomes of spawning and prespawning sea lamprey in both sexes using both non-targeted and targeted metabolomics approaches using UPLC/MS-MS with electrospray ionization in both positive and negative modes. The data were processed using Progenesis QI, Compound Discoverer and XCMS softwares for alignment, peak picking, and deconvolution of the peaks. Principle component analyses (PCA) and partial least squares discriminant analyses (PLS-DA) were performed using SIMCA and Metaboanalyst softwares to identify discriminating features, followed by fragmentation matching with extensive database search and pathway mapping. ResultsThe pheromonal bile acid biosynthesis was upregulated signi cantly in males compared to females. Spermiating males further upregulated bile acid biosynthesis by altering amino acid metabolisms, upregulating cofactors and nucleotide metabolisms, but downregulating carbohydrate and energy metabolisms.Conclusion Plasma metabolomes are sex-and maturation-dependent and re ect the special metabolic demands at each life stage and reproductive strategy.

show abstract

Muscle fatty acid profiles of sea lamprey (Petromyzon marinus L.) indicate the use of fast metabolized energy during ontogenesis

Cited by 5 publications

References 76 publications

Are lipids always depleted? Comparison of hydrogen, carbon, and nitrogen isotopic values in the muscle and lipid of larval lampreys

Are lipids always depleted? Comparison of hydrogen, carbon, and nitrogen isotopic values in the muscle and lipid of larval lampreys

Are lipids always light? Lipids in larval lampreys are enriched in 13C but depleted in 2H relative to muscle

Plasma metabolomic profiles reveal sex- and maturation-dependent metabolic strategies in sea lamprey (Petromyzon marinus)

Contact Info

Product

Resources

About