Muscle Extracellular Matrix Scaffold Is a Multipotent Environment

Aulino, Paola; Costa, Alessandra; Chiaravalloti, Ernesto; Perniconi, Barbara; Adamo, Sergio; Coletti, Dario; Marrelli, Massimo; Tatullo, Marco; Teodori, Laura

doi:10.7150/ijms.10761

Cited by 48 publications

(36 citation statements)

References 15 publications

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“…Although the mechanisms are not yet fully understood, paracrine secreted cytokines and/or the extracellular matrix might affect the differentiation [ 11 ]. Muscle extracellular matrix scaffolds can recruit stem cells and induce differentiation, and this extracellular matrix scaffold promotes regeneration of other tissues such as bone cells depending on the surrounding environment [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Supplementation with IL-6 and Muscle Cell Culture Conditioned Media Enhances Myogenic Differentiation of Adipose Tissue-Derived Stem Cells through STAT3 Activation

Seo

Kang

Lim

et al. 2018

IJMS

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Mature skeletal muscle cells cannot be expanded in culture systems. Therefore, it is difficult to construct an in vitro model for muscle diseases. To establish an efficient protocol for myogenic differentiation of human adipose tissue-derived stem cells (hADSCs), we investigated whether addition of IL-6 and/or myocyte-conditioned media (CM) to conventional differentiation media can shorten the differentiation period. hADSCs were differentiated to myocytes using the conventional protocol or modified with the addition of 25 pg/mL IL-6 and/or C2C12 CM (25% v/v). The expression of MyoD and myogenine mRNA was significantly higher at 5–6 days after differentiation using the modified protocol than with the conventional protocol. mRNA and protein expression of myosin heavy chain, a marker of myotubes, was significantly upregulated at 28 and 42 days of differentiation using the modified protocol, and the level achieved after a 4-week differentiation period was similar to that achieved at 6 weeks using the conventional protocol. The expression of p-STAT3 was significantly increased when the modified protocol was used. Similarly, addition of colivelin, a STAT3 activator, instead of IL-6 and C2C12 CM, promoted the myogenic differentiation of ADSCs. The modified protocol improved differentiation efficiency and reduced the time required for differentiation of myocytes. It might be helpful to save cost and time when preparing myocytes for cell therapies and drug discovery.

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Section: Discussionmentioning

confidence: 99%

Supplementation with IL-6 and Muscle Cell Culture Conditioned Media Enhances Myogenic Differentiation of Adipose Tissue-Derived Stem Cells through STAT3 Activation

Seo

Kang

Lim

et al. 2018

IJMS

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“…The cell niche is a concept relatively new 18 and many authors have demonstrated that a scaffold can be multipotent 19 and can induce different phenotypes. On the other hand, cells can follow their own basal commitment 20 or they can be induced to differentiate into a phenotype definitely different from the biological niche where they were harvested.…”

Section: Discussionmentioning

confidence: 99%

Mechanical influence of tissue culture plates and extracellular matrix on mesenchymal stem cell behavior: A topical review

Tatullo

Marrelli

Falisi

et al. 2015

Int J Immunopathol Pharmacol

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Tissue engineering applications need a continuous development of new biomaterials able to generate an ideal cellextracellular matrix interaction. The stem cell fate is regulated by several factors, such as growth factors or transcription factors. The most recent literature has reported several publications able to demonstrate that environmental factors also contribute to the regulation of stem cell behavior, leading to the opinion that the environment plays the major role in the cell differentiation.The interaction between mesenchymal stem cells (MSCs) and extracellular environment has been widely described, and it has a crucial role in regulating the cell phenotype. In our laboratory (Tecnologica Research Institute, Crotone, Italy), we have recently studied how several physical factors influence the distribution and the morphology of MSCs isolated from dental pulp, and how they are able to regulate stem cell differentiation. Mechanical and geometrical factors are only a small part of the environmental factors able to influence stem cell behavior, however, this influence should be properly known: in fact, this assumption must be clearly considered during those studies involving MSCs; furthermore, these interactions should be considered as an important bias that involves an high number of studies on the MSCs, since in worldwide laboratories the scientists mostly use tissue culture plates for their experiments.

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“…In recent years, regenerative medicine issues have stimulated research to investigate an elevated number of new biomatrices 4 aimed at improving tissue healing; in this light, the old concept of bioscaffolds has given way to new concepts, such as acellular scaffolds, 5 cell niche, and cell commitment. [6][7][8][9][10] Regarding the scaffolds, the exposed surface area and porosity play major roles in the proliferative response of cultured cells. 11 Moreover, the architecture and the design of scaffolds have a clear effect on cells seeding and culturing.…”

Section: Discussionmentioning

confidence: 99%

Innovative approach for the in vitro research on biomedical scaffolds designed and customized with CAD-CAM technology

Marrelli

Pujia

Palmieri

et al. 2016

Int J Immunopathol Pharmacol

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Introduction: Studies on biomaterials involve assays aimed to assess the interactions between the biomaterial and the cells seeded on its surface. However, the morphology of biomaterials is heterogeneous and it could be tricky to standardize the results among different biomaterials and the classic plastic plates. In this light, we decided to create, by means of computer-aided design (CAD) technology, a standardized sample model, with equal shape and sizes, able to fit into a classic shape of a 96-wells tissue culture plate (TCP). Methods:The design of this sample consists of a hole in the top in order to allow the injected cells to settle without them being able to slip from the sides of the sample to the bottom of the TCP wells. This CAD project is made using the software Pro-Engineer. The sample will totally fill the wells of the 96-well TCP. Dental pulp stem cells have been used to assess the ability of the different sample to support and promote the cell proliferation. Results: Twelve titanium, 12 gold-palladium, and 12 zirconium oxide customized samples were designed by means of the software cam powermill, by importing the .stl file created in Pro-Engineer software. The proliferation rate of the tested scaffolds showed to be similar to the control in the group with the customized shape. Conclusion: We think that our method can be useful to test different types of scaffolds when a greater accuracy of the measurements is desirable in order to verify the cell behavior of these scaffolds. Our innovative method can improve the standardization process in the evaluation of cell behavior on different biomaterials to open the way to more reliable tests on biomatrices functionalized with drugs or growth factors applied to the future regenerative medicine.

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Muscle Extracellular Matrix Scaffold Is a Multipotent Environment

Cited by 48 publications

References 15 publications

Supplementation with IL-6 and Muscle Cell Culture Conditioned Media Enhances Myogenic Differentiation of Adipose Tissue-Derived Stem Cells through STAT3 Activation

Supplementation with IL-6 and Muscle Cell Culture Conditioned Media Enhances Myogenic Differentiation of Adipose Tissue-Derived Stem Cells through STAT3 Activation

Mechanical influence of tissue culture plates and extracellular matrix on mesenchymal stem cell behavior: A topical review

Innovative approach for the in vitro research on biomedical scaffolds designed and customized with CAD-CAM technology

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