Muscle does not drive persistent posttraumatic elbow contracture in a rat model

Dunham, Chelsey L.; Chamberlain, Aaron M.; Meyer, Gretchen A.; Lake, Spencer P.

doi:10.1002/mus.26344

Cited by 10 publications

(9 citation statements)

References 37 publications

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“…5 and 7). These results were also consistent with previous data that showed active and passive muscle mechanics were not significantly different compared with control at 42/42 IM-FM 14 . Similarly, studies of immobilization-induced knee contracture animal models have demonstrated that the contribution of muscles/tendons after immobilization decreased with time [4][5][6] .…”

Section: Discussionsupporting

confidence: 93%

“…This limited yet significant contribution after immobilization was consistent with the findings of our previous study, which showed that active and passive muscle mechanics were significantly altered at 42 IM (Figs. 3-B and 5) 14 . Thus, muscle contributes to early elbow contracture.…”

Section: Discussionmentioning

confidence: 99%

“…The objective of this study was to evaluate the passive contributions of muscles/tendons, capsule, and ligaments/cartilage to loss of elbow extension caused by contracture. We hypothesized that all tissues would substantially contribute to elbow contracture after immobilization, but that only the capsule, ligaments, and cartilage would contribute after free mobilization because we previously reported that altered muscle mechanics recovered after free mobilization 14 . At all time points, we hypothesized that the capsule would be the primary contributor to contracture on the basis of persistent biological changes observed in human patients 2,[8][9][10] and in our rat model 13 .…”

mentioning

confidence: 99%

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The Role of Periarticular Soft Tissues in Persistent Motion Loss in a Rat Model of Posttraumatic Elbow Contracture

Dunham

Castile

Chamberlain

et al. 2019

The Journal of Bone and Joint Surgery

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Background: Elbow injuries disrupt the surrounding periarticular soft tissues, which include the muscles, tendons, capsule, ligaments, and cartilage. Damage to these tissues as a result of elbow trauma causes clinically significant contracture in 50% of patients. However, it is unclear which of these tissues is primarily responsible for the decreased range of motion. We hypothesized that all tissues would substantially contribute to elbow contracture after immobilization, but only the capsule, ligaments, and cartilage would contribute after free mobilization, with the capsule as the primary contributor at all time points. Methods: Utilizing a rat model of posttraumatic elbow contracture, a unilateral soft-tissue injury was surgically induced to replicate the damage that commonly occurs during elbow joint dislocation. After surgery, the injured limb was immobilized for 42 days. Animals were evaluated after either 42 days of immobilization (42 IM) or 42 days of immobilization with an additional 21 or 42 days of free mobilization (42/21 or 42/42 IM-FM). For each group of animals, elbow mechanical testing in flexion-extension was completed post-mortem with (1) all soft tissues intact, (2) muscles/tendons removed, and (3) muscle/tendons and anterior capsule removed. Total extension was assessed to determine the relative contributions of muscles/tendons, capsule, and the remaining intact tissues (i.e., ligaments and cartilage). Results: After immobilization, the muscles/tendons and anterior capsule contributed 10% and 90% to elbow contracture, respectively. After each free mobilization period, the muscles/tendons did not significantly contribute to contracture. The capsule and ligaments/cartilage were responsible for 47% and 52% of the motion lost at 42/21 IM-FM, respectively, and 26% and 74% at 42/42 IM-FM, respectively. Conclusions: Overall, data demonstrated a time-dependent response of periarticular tissue contribution to elbow contracture, with the capsule, ligaments, and cartilage as the primary long-term contributors. Clinical Relevance: The capsule, ligaments, and cartilage were primarily responsible for persistent motion loss and should be considered during development of tissue-targeted treatment strategies to inhibit elbow contracture following injury. Disclosure: Funding was provided by the National Institutes of Health (NIBIB T32 EB018266 and NIAMS R03 ARR067504) but did not play a role in the investigation. On the Disclosure of Potential Conflicts of Interest forms, which are provided with the online version of the article, one or more of the authors checked "yes" to indicate that the author had a relevant financial relationship in the biomedical arena outside the submitted work (http://links. lww.com/JBJS/F85).

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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The Role of Periarticular Soft Tissues in Persistent Motion Loss in a Rat Model of Posttraumatic Elbow Contracture

Dunham

Castile

Chamberlain

et al. 2019

The Journal of Bone and Joint Surgery

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“…This study investigated sex-based differences in an established rat model of elbow PTJC where previous work evaluated only male animals. [5][6][7][8][9][10][11] While previous work investigated temporal changes in PTJC, 8 this study focused on evaluating differences between sexes.…”

Section: Discussionmentioning

confidence: 99%

“…to contracture have also been evaluated. [10][11][12] However, these previous studies that investigated disease etiology, progression and recovery were conducted only with male animals; it remains unknown whether female animals would exhibit similar responses.…”

Section: Introductionmentioning

confidence: 99%

Females and males exhibit similar functional, mechanical, and morphological outcomes in a rat model of posttraumatic elbow contracture

Reiter

Schott

Castile

et al. 2020

Journal Orthopaedic Research

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Posttraumatic joint contracture (PTJC) is a debilitating condition characterized by loss of joint motion following injury. Previous work in a rat model of elbow PTJC investigated disease etiology, progression, and recovery in only male animals; this study explored sex-based differences. Rat elbows were subjected to a unilateral anterior capsulotomy and lateral collateral ligament transection followed by 42 days of immobilization and 42 days of free mobilization. Grip strength and gait were collected throughout the free mobilization period while joint mechanical testing, microcomputed tomography and histological analysis were performed postmortem. Overall, few differences were seen between sexes in functional, mechanical, and morphological outcomes with PTJC being similarly debilitating in male and female animals. Functional measures of grip strength and gait showed that, while some baseline differences existed between sexes, traumatic injury produced similar deficits that remained significantly different long-term when compared to control animals. Similarly, male and female animals both had significant reductions in joint range of motion due to injury. Ectopic calcification (EC), which had not been previously evaluated in this injury model, was present in all limbs on the lateral side. Injury caused increased EC volume but did not alter mineral density regardless of sex.Furthermore, histological analysis of the anterior capsule showed minor differences between sexes for inflammation and thickness but not for other histological parameters. A quantitative understanding of sex-based differences associated with this injury model will help inform future therapeutics aimed at reducing or preventing elbow PTJC.

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Direct measurement of the direction-dependent mechanical behaviour of skeletal muscle extracellular matrix

et al. 2021

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Muscle does not drive persistent posttraumatic elbow contracture in a rat model

Abstract: Muscle is a transient contributor to motion loss in our rat model of posttraumatic elbow contracture. Muscle Nerve, 2018.

Cited by 10 publications

References 37 publications

The Role of Periarticular Soft Tissues in Persistent Motion Loss in a Rat Model of Posttraumatic Elbow Contracture

The Role of Periarticular Soft Tissues in Persistent Motion Loss in a Rat Model of Posttraumatic Elbow Contracture

Females and males exhibit similar functional, mechanical, and morphological outcomes in a rat model of posttraumatic elbow contracture

Direct measurement of the direction-dependent mechanical behaviour of skeletal muscle extracellular matrix

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