Muscle Degeneration: Preparation of Stretchable Nanofibrous Sheets with Sacrificial Coaxial Electrospinning for Treatment of Traumatic Muscle Injury (Adv. Healthcare Mater. 8/2021)

A Self‐Renewing Biomimetic Skeletal Muscle Construct Engineered using Induced Myogenic Progenitor Cells

“…[10] As a consequence of these attributes, electrospun substrates have been utilized to produce highly aligned myotubes using multiple materials including decellularized extracellular matrix (ECM), [21,22] synthetic or natural polymers such as polyurethane, [23] poly(ɛ-caprolactone) (PCL), [27] poly(lactic-co-glycolic acid), [19] fibrin/alginate, [25] and gelatin. [11,26] However, incorporation of self-renewing muscle stem cells into electrospun nano-scaled fibers that can regenerate muscle fibers has seldom been reported. [10,11] To date, a variety of myogenic cells have been used for skeletal muscle tissue engineering purposes, predominantly immortalized myoblasts such as C2C12 or primary myoblasts.…”

Reactive Oxygen Species (ROS)‐Assisted Nano‐Therapeutics Surface‐Decorated with Epidermal Growth Factor Fragments for Enhanced Wound Healing

“…[ 13,16,17 ] We previously demonstrated the potential of polycaprolactone (PCL) nanofibers coated with gelatin in wound healing by co‐culturing them with stem cells to create a cell‐nanofiber composite. [ 18 ] When this composite was applied to a full‐thickness wound on the back of a mouse, wound recovery was 1.26‐times faster compared with the non‐treated mouse. In addition, gelatin could be directly electrospun into nanofiber to treat traumatic muscle injuries, resulting in a 1.29 times increase in the limb weight after 4 weeks of treatment compared to non‐treated group, and the immunostaining results confirmed well‐recovered muscle tissue.…”

“…In addition, gelatin could be directly electrospun into nanofiber to treat traumatic muscle injuries, resulting in a 1.29 times increase in the limb weight after 4 weeks of treatment compared to non‐treated group, and the immunostaining results confirmed well‐recovered muscle tissue. [ 18 ] Nanofibrous matrix prepared from collagen was also reported to enhance cell adhesion and proliferation for early‐stage wound healing, resulting in 2.23‐fold and 2.07‐fold increases, respectively, after 18 h‐application of nanofibrous matrix to human oral keratinocytes. [ 19 ] In our previous study, EGF‐conjugated nanofiber was investigated its efficacy in promoting wound healing.…”

“…[10][11][12] Among different strategies to develop EGF-contained biomaterials, electrospun scaffolds have become popular as they can support 3D cell cultivation to imitate the structure of extracellular substrates. [13][14][15] Therefore, numerous studies have been conducted to explore the potential of fibrous matrices as biomedical devices to promote cell proliferation, particularly in wound healing applications. [13,16,17] We previously demonstrated the potential of polycaprolactone (PCL) nanofibers coated with gelatin in wound healing by co-culturing them with stem cells to create a cell-nanofiber composite.…”