Muscle cell proliferation using water-soluble extract from nitrogen-fixing cyanobacteria Anabaena sp. PCC 7120 for sustainable cultured meat production

Microbiota-Derived Postbiotics Enhance the Proliferative Effects of Growth Factors on Satellite Cells in Cultivated Meat Applications

“…Similar to our approach, algae extract obtained from Chlorella vulgaris [31] and cyanobacteria extracts derived from Spirulina [30] have been used as a partial FBS and GFs replacement in mesenchymal stem cells and cell cultures, respectively. In another study conducted by Ghosh et al (2023), bioactive compound was extracted from the filamentous nitrogen-fixing cyanobacteria Anabaena sp. PCC 7120 by the ultrasonication method and used as a growth supplement to cultivate muscle cells.…”

“…PCC 7120 by the ultrasonication method and used as a growth supplement to cultivate muscle cells. Their results indicated that water-soluble compounds had a positive role in muscle cell proliferation [15]. Although several compounds have been clarified in these extracts such as phenolic compounds and proteins, it is still unknown which compounds or molecules are responsible for stimulation of the cell growth [37].…”

“…The cDNAs were synthesized with the iScript cDNA Synthesis Kit (BioRad, #1708891), following the manufacturer's guidelines. Reaction ingredients [0.5 µL of cDNA, 5 µL of SsoAdvanced Universal SYBR Green Supermix (BioRad, #1725270), and 10 µM from each primer pair listed in Table 1] were mixed for qRT-PCR analysis and run in LightCycler ® 480 (Roche, Basel, Switzerland) [15,16]. The gene expression levels relative to the housekeeping gene (i.e., Rps15a) were calculated using the 2 −∆Ct formula.…”

“…Therefore, novel strategies for manufacturing of culture meat at a reduced cost by replacing GFs with less expensive ones have still gained attention. Nowadays, researchers are looking to replace growth factors with plant-based alternatives [14] or with bioactive compounds such as cyanobacteria [15] and algae extracts [8].…”