Muscle and Liver Protein Synthesis Adapt Efficiently to Food Deprivation and Refeeding in 12-Month-Old Rats

Mosoni, Laurent; Malmezat, T.; Valluy, Marie-Claude; Houlier, Marie-Louise; Mirand, Philippe Patureau

doi:10.1093/jn/126.2.516

Cited by 29 publications

(32 citation statements)

References 24 publications

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“…There was a Ͻ10% difference between free valine enrichments in plasma, gastrocnemius, and liver (88-89%, 84-87%, and 78-85% of injected valine, respectively). We also demonstrated that free valine enrichment in plasma reached 89% of the plateau value within 5 min after injection and remained stable for more than 45 min (23). These measurements were made between 1:30 PM and 4:30 PM in fed or refed rats (postprandial state) and between 9 AM and 11 AM in starved rats.…”

Section: Methodssupporting

confidence: 51%

“…We used male Sprague-Dawley rats (obtained from Iffa Credo, Lyon, France) aged 12 or 24 mo at the end of the experimentation. They were maintained in individual metabolism cages at 22°C with a 12:12-h lightdark cycle (lights on from 9 PM to 9 AM) with free access to water; they were weighed daily and were offered a semiliquid casein-based diet containing 18.7 g protein/100 g dry matter, as described previously (23). The animals had free access to food between 9 AM and 5 PM.…”

Section: Methodsmentioning

confidence: 99%

“…Our aim was to induce a significant loss of muscle proteins to be able to analyze recovery. We first used 4-day starvation, but no significant loss occurred (23). We had to use 10-day starvation to obtain a significant but moderate…”

Section: Methodsmentioning

confidence: 99%

“…Protein synthesis rates were measured according to the flooding-dose method (10), which reduces uncertainty over the labeling of the tracer amino acid in the precursor pool for protein synthesis. The method was adapted for mature rats as described previously (23): each rat was injected subcutaneously with a flooding dose of valine (300 µmol/100 g body wt) to flood the precursor pools, with 50% L-[1-13 C]valine (MassTrace, Woburn, MA). The incorporation time was 40 min.…”

Section: Methodsmentioning

confidence: 99%

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Lower recovery of muscle protein lost during starvation in old rats despite a stimulation of protein synthesis

Mosoni

Malmezat

Valluy

et al. 1999

American Journal of Physiology-Endocrinology and Metabolism

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Sarcopenia could result from the inability of an older individual to recover muscle lost during catabolic periods. To test this hypothesis, we compared the capacity of 5-day-refed 12- and 24-mo-old rats to recover muscle mass lost after 10 days without food. We measured gastrocnemius and liver protein synthesis with the flooding-dose method and also measured nitrogen balance, 3-methylhistidine excretion, and the gene expression of components of proteolytic pathways in muscle comparing fed, starved, and refed rats at each age. We show that 24-mo-old rats had an altered capacity to recover muscle proteins. Muscle protein synthesis, inhibited during starvation, returned to control values during refeeding in both age groups. The lower recovery in 24-mo-old rats was related to a lack of inhibition of muscle proteolysis during refeeding. The level of gene expression of components of the proteolytic pathways did not account for the variations in muscle proteolysis at both ages. In conclusion, this study highlights the role of muscle proteolysis in the lower recovery of muscle protein mass lost during catabolic periods.

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Section: Methodssupporting

confidence: 51%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Lower recovery of muscle protein lost during starvation in old rats despite a stimulation of protein synthesis

Mosoni

Malmezat

Valluy

et al. 1999

American Journal of Physiology-Endocrinology and Metabolism

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show abstract

“…However, feeding has little effect on muscle protein synthesis in adult mammals (Melville et al 1989;Baillie and Garlick, 1992;Tessari et al 1996). By contrast, feeding stimulates liver protein synthesis in both growing (Burrin et al 1992 and adult (Preedy et al 1988;Mosoni et al 1996) animals.…”

Section: Protein Deposition Is Developmentallymentioning

confidence: 92%

Interaction of amino acids and insulin in the regulation of protein metabolism in growing animals

Davis

Suryawan²,

Bush³

et al. 2003

Can. J. Anim. Sci.

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Connor, P. M. and Thivierge, M. C. 2003. Interaction of amino acids and insulin in the regulation of protein metabolism in growing animals. Can. J. Anim. Sci. 83: 357-364. Young animals utilize their dietary amino acids more efficiently for growth because they are capable of a greater increase in tissue protein synthesis in response to feeding than older animals. This response to feeding is particularly profound in skeletal muscle. The feeding-induced stimulation of protein synthesis in skeletal muscle is uniquely and independently regulated by both insulin and amino acids. In most visceral tissues, the stimulation of protein synthesis by feeding is mediated by amino acids alone and not by insulin. The stimulation of protein synthesis by nutrition and hormones is regulated by alterations in the expression and activity of components of the intracellular signaling pathways that control the initiation of translation. Les jeunes animaux utilisent mieux les acides aminés des aliments pour la croissance parce que leurs tissus synthétisent plus de protéines que ceux de leurs aînés. Cette réaction est particulièrement évidente dans le muscle squelettique. La stimulation de la synthèse des protéines par les aliments dans le muscle squelettique est particulière et est régulée de manière indépendante par l'insuline et les acides aminés. Dans la plupart des viscères, cette stimulation n'est actionnée que par les acides aminés des aliments, pas l'insuline. La stimulation de la synthèse des protéines par la nutrition et les hormones est contrôlée par un changement au niveau de l'expression et de l'activité de certains éléments des systèmes intracellulaires qui amorcent la traduction des signaux.

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Decreased glutamate, glutamine and citrulline concentrations in plasma and muscle in endotoxemia cannot be reversed by glutamate or glutamine supplementation: a primary intestinal defect?

Boutry

Matsui²,

Bos

et al. 2012

Amino Acids

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Endotoxemia affects intestinal physiology. A decrease of circulating citrulline concentration is considered as a reflection of the intestinal function. Citrulline can be produced in enterocytes notably from glutamate and glutamine. The aim of this work was to determine if glutamate, glutamine and citrulline concentrations in blood, intestine and muscle are decreased by endotoxemia, and if supplementation with glutamate or glutamine can restore normal concentrations. We induced endotoxemia in rats by an intraperitoneal injection of 0.3 mg kg(-1) lipopolysaccharide (LPS). This led to a rapid anorexia, negative nitrogen balance and a transient increase of the circulating level of IL-6 and TNF-α. When compared with the values measured in pair fed (PF) animals, almost all circulating amino acids (AA) including citrulline decreased, suggesting a decrease of intestinal function. However, at D2 after LPS injection, most circulating AA concentrations were closed to the values recorded in the PF group. At that time, among AA, only glutamate, glutamine and citrulline were decreased in gastrocnemius muscle without change in intestinal mucosa. A supplementation with 4% monosodium glutamate (MSG) or an isomolar amount of glutamine failed to restore glutamate, glutamine and citrulline concentrations in plasma and muscle. However, MSG supplementation led to an accumulation of glutamate in the intestinal mucosa. In conclusion, endotoxemia rapidly but transiently decreased the circulating concentrations of almost all AA and more durably of glutamate, glutamine and citrulline in muscle. Supplementation with glutamate or glutamine failed to restore glutamate, glutamine and citrulline concentrations in plasma and muscles. The implication of a loss of the intestinal capacity for AA absorption and/or metabolism in endotoxemia (as judged from decreased citrulline plasma concentration) for explaining such results are discussed.

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Muscle and Liver Protein Synthesis Adapt Efficiently to Food Deprivation and Refeeding in 12-Month-Old Rats

Cited by 29 publications

References 24 publications

Lower recovery of muscle protein lost during starvation in old rats despite a stimulation of protein synthesis

Lower recovery of muscle protein lost during starvation in old rats despite a stimulation of protein synthesis

Interaction of amino acids and insulin in the regulation of protein metabolism in growing animals

Decreased glutamate, glutamine and citrulline concentrations in plasma and muscle in endotoxemia cannot be reversed by glutamate or glutamine supplementation: a primary intestinal defect?

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