MusaMPK5, a mitogen activated protein kinase is involved in regulation of cold tolerance in banana

Tak, Himanshu; Negi, Sanjana; Rajpurohit, Yogendra S.; Misra, Hari S.; Ganapathi, T. R.

doi:10.1016/j.plaphy.2019.11.012

Cited by 21 publications

(11 citation statements)

References 39 publications

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“…Multiple reports delineate isolation and functional characterization of stress-inducible promoters from varied plant species (Ben Saad et al, 2020;Divya et al, 2019;Srinath et al, 2017;Swapna et al, 2011). A few reports on the characterization of the promoter regions of stress-responsive genes and xylem specific transcription factors have also recently emerged in banana plants (Negi et al, 2017;Negi et al, 2018b;Negi & TakH, 2019;Tak et al, 2018;Tak et al, 2020). Despite that, only few stress-related promoters have been characterized for their responses in different organs of plant towards multiple stress conditions (Yamaguchi- Shinozaki & Shinozaki, 1993).…”

Section: Discussionmentioning

confidence: 99%

“…Two-month-old plants in greenhouse were subjected to either high-salinity (250 mM NaCl), drought (dehydrating on blotting paper), cold (4 ± 2 C) or applications of methyl jasmonate (200 μM MeJA), salicylic acid (2 mM), and ABA (100 μM) for defined time points (ranging from 1 to 24 h). In different experiments, equal volume of hormonal (MeJA, SA, and ABA) and sodium chloride solutions were either sprayed or used to irrigate the plants (Negi et al, 2016;Tak et al, 2020). Leaves from treated plants were collected and total RNA was isolated immediately with the help of Concert plant RNA-reagent (Invitrogen) and RNeasy plant mini kit (Qiagen) as described previously (Tak et al, 2017).…”

Section: Expression Profiling Of Musawrky18 In Banana Plantsmentioning

confidence: 99%

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The 5′‐upstream region of WRKY18 transcription factor from banana is a stress‐inducible promoter with strong expression in guard cells

Tak

Negi

Ganapathi

2021

Physiologia Plantarum

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Increasing crop productivity in an ever-changing environmental scenario is a major challenge for maintaining the food supply worldwide. Generation of crops having broad-spectrum pathogen resistance with the ability to cope with water scarcity is the only solution to feed the expanding world population. Stomatal closure has implications on pathogen colonization and drought tolerance. Recent studies have provided novel insights into networks involved in stomatal closure which is being used in biotechnological applications for improving crop endurance. Despite that genetic engineering of stomata requires guard cell preferred or specific regulatory regions to avoid undesirable side effects. In the present study, we describe the 5 0 -upstream regulatory region of the WRKY18 transcription factor of banana and functionally analyzed its stress meditated activation and strong guard cell preferred activity. Expression ofMusaWRKY18 is augmented in leaves of banana cultivars Karibale Monthan, Rasthali and Grand Nain under multiple stress conditions suggesting its role in stress responses of banana plants. Transgenic tobacco lines harboring P MusaWRKY18β-D-glucuronidase (GUS) were regenerated and GUS staining demonstrated substantial GUS expression in guard cells which corroborates with multiple Dof1 binding cis-elements in P MusaWRKY18 . Fluorescent β-galactosidase assay demonstrated the stress-mediated strong induction profiles of P MusaWRKY18 at different time points in transgenic tobacco lines exposed to drought, high-salinity, cold, and applications of abscisic acid, salicylic acid, methyl jasmonate, and ethephon. This study sheds novel insights into guard cell preferred expression of WRKY genes under stress and confirm the utility of P MusaWRKY18 for exploring guard cell functions and guard cell engineering.

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Section: Discussionmentioning

confidence: 99%

Section: Expression Profiling Of Musawrky18 In Banana Plantsmentioning

confidence: 99%

The 5′‐upstream region of WRKY18 transcription factor from banana is a stress‐inducible promoter with strong expression in guard cells

Tak

Negi

Ganapathi

2021

Physiologia Plantarum

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“…Antarctica represents one of the coldest environments, and low temperature is the fundamental property in Antarctic area. MAPKs are widely known to act as vital functions in stress responses of plants [33], animals [34] and yeasts [35], and then activate other functional proteins to accommodate low temperature stress. In yeast Dekkera bruxellensis, HOG-MAPK signaling pathway was activated in cold stress response [35].…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning and sequence analysis of a mitogen-activated protein kinase gene in the Antarctic yeast Rhodotorula mucilaginosa AN5

Shi

Zhang

et al. 2021

Mol Biol Rep

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The mitogen-activated protein kinase (MAPK) cascades play important roles in various signaling transduction networks of biotic and abiotic stress responses. However, MAPK signaling pathways in cold-active yeast Rhodotorula mucilaginosa have not been reported comprehensively. In the present study, MAPK gene (RmMAPK) was first cloned and characterized from Antarctic sea ice yeast R. mucilaginosa AN5. The full length of the RmMAPK gene is 1086 bp and encodes a 361 amino acids protein with a predicted molecular mass of 40.9 kDa and a pI of 5.25. The RmMAPK contains 11 MAPK conserved subdomains and the phosphorylation motif TGY located in the activation loop of the kinase. Quantitative real-time PCR assay revealed that the expression of RmMAPK up-regulated rapidly and significantly when yeast cells were subjected to low temperature (4 °C ), high salinity (120‰ NaCl) and heavy metal (2 mmol/L CuCl2), which suggested that the RmMAPK might act as a key function in response to extreme stresses, such as low temperature, high salinity and heavy metal.

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“…These genes might regulate a range of ABA effector proteins via phosphorylation (De Zelicourt et al, 2016) and may also play major roles in the regulation of cell growth as well as cellular biotic and abiotic stress signaling (Lammers and Lavi, 2007;Fan et al, 2019). Proteins belonging to MAPK cascades have also been shown to be involved in the response of plants to cold stress (Markus et al, 2004;Tak et al, 2019), while PTP1 is known to downregulate cold stress (Liu et al, 2016). Thus, we propose that the ABA signaling pathway via PP2C is most important in terms of the cold stress responses of S. spontaneum at the seedling stage; however, future research should be conducted on this pathway.…”

Section: Transcriptional Regulatory Network Underlying Cold Stress At the Seedling Stagementioning

confidence: 99%

Integration of Transcriptional and Post-transcriptional Analysis Revealed the Early Response Mechanism of Sugarcane to Cold Stress

et al. 2021

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Cold stress causes major losses to sugarcane production, yet the precise molecular mechanisms that cause losses due to cold stress are not well-understood. To survey miRNAs and genes involved in cold tolerance, RNA-seq, miRNA-seq, and integration analyses were performed on Saccharum spontaneum. Results showed that a total of 118,015 genes and 6,034 of these differentially expressed genes (DEGs) were screened. Protein–protein interaction (PPI) analyses revealed that ABA signaling via protein phosphatase 2Cs was the most important signal transduction pathway and late embryogenesis abundant protein was the hub protein associated with adaptation to cold stress. Furthermore, a total of 856 miRNAs were identified in this study and 109 of them were differentially expressed in sugarcane responding to cold stress. Most importantly, the miRNA–gene regulatory networks suggested the complex post-transcriptional regulation in sugarcane under cold stress, including 10 miRNAs−42 genes, 16 miRNAs−70 genes, and three miRNAs−18 genes in CT vs. LT0.5, CT vs. LT1, and CT0.5 vs. LT1, respectively. Specifically, key regulators from 16 genes encoding laccase were targeted by novel-Chr4C_47059 and Novel-Chr4A_40498, while five LRR-RLK genes were targeted by Novel-Chr6B_65233 and Novel-Chr5D_60023, 19 PPR repeat proteins by Novel-Chr5C_57213 and Novel-Chr5D_58065. Our findings suggested that these miRNAs and cell wall-related genes played vital regulatory roles in the responses of sugarcane to cold stress. Overall, the results of this study provide insights into the transcriptional and post-transcriptional regulatory network underlying the responses of sugarcane to cold stress.

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MusaMPK5, a mitogen activated protein kinase is involved in regulation of cold tolerance in banana

Cited by 21 publications

References 39 publications

The 5′‐upstream region of WRKY18 transcription factor from banana is a stress‐inducible promoter with strong expression in guard cells

The 5′‐upstream region of WRKY18 transcription factor from banana is a stress‐inducible promoter with strong expression in guard cells

Molecular cloning and sequence analysis of a mitogen-activated protein kinase gene in the Antarctic yeast Rhodotorula mucilaginosa AN5

Integration of Transcriptional and Post-transcriptional Analysis Revealed the Early Response Mechanism of Sugarcane to Cold Stress

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