Murraya koenigii leaf extract inhibits proteasome activity and induces cell death in breast cancer cells

Noolu, Bindu; Ajumeera, Rajanna; Chauhan, Anitha; Balakrishna, Nagalla; Raghunath, Manchala; Ismail, Azman

doi:10.1186/1472-6882-13-7

Cited by 50 publications

(41 citation statements)

References 37 publications

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“…In particular, the 26S protease regulatory subunit 10B is a protein associated with apoptosis, and disappears in the floating cells. The decreased activity of the 26S proteasome and induction of cell death was observed only in breast cancer cells and not in normal cells, treated with Murraya koenigii leaf extract (37). The western blot of the present study also demonstrated that expression of hnRNP H and hnRNP A2/B1 disappeared completely subsequent to apigenin treatment.…”

Section: Discussionsupporting

confidence: 52%

Apigenin inhibits growth and induces apoptosis in human cholangiocarcinoma cells

et al. 2017

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Abstract.A promising nutraceutical, apigenin, was recently revealed to exhibit biological activity in inhibiting several types of cancer. The effects of apigenin on the growth inhibition and apoptosis of the cholangiocarcinoma HuCCA-1 cell line were investigated. Protein alterations subsequent to apigenin treatment were studied using a proteomic approach. The values of 20, 50 and 90% inhibition of cell growth (IC 20 , IC 50 and IC 90 ) were determined by MTT cell viability assay. Apoptotic cell death was detected using two different methods, a flow cytometric analysis (Muse Cell Analyzer) and DNA fragmentation assay. A number of conditions including attached and detached cells were selected to perform two-dimensional gel electrophoresis (2-DE) to study the alterations in the expression levels of treated and untreated proteins and identified by liquid chromatography (LC)/tandem mass spectrometry (MS/MS). The IC 20 , IC 50 and IC 90 values of apigenin after 48 h treatment in HuCCA-1 cells were 25, 75 and 200 µM, respectively, indicating the cytotoxicity of this compound. Apigenin induced cell death in HuCCA-1 cells via apoptosis as detected by flow cytometric analysis and exhibited, as confirmed with DNA fragmentation, characteristics of apoptotic cells. A total of 67 proteins with altered expression were identified from the 2-DE analysis and LC/MS/MS. The cleavage of proteins involved in cytoskeletal, cytokeratin 8, 18 and 19, and high expression of S100-A6 and S100-A11 suggested that apoptosis was induced by apigenin via the caspase-dependent pathway. Notably, two proteins, heterogeneous nuclear ribonucleoprotein H and A2/B1, disappeared completely subsequent to treatment, suggesting the role of apigenin in inducing cell death. The present study indicated that apigenin demonstrates an induction of growth inhibition and apoptosis in cholangiocarcinoma cells and the apoptosis pathway was confirmed by proteomic analysis.

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Section: Discussionsupporting

confidence: 52%

Apigenin inhibits growth and induces apoptosis in human cholangiocarcinoma cells

et al. 2017

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“…Increased activation in the DPPH assay from the optimized extracts corroborates these earlier findings. The anticancer activity of curry leaf extracts against MDA-MB-231 and MCF-7 cell lines (breast cancer) has been reported previously [15, 29, 30]. The anticancer activity of curry leaves against HeLa cancer cells and the effective dose of this extract have been scarcely reported.…”

Section: Resultsmentioning

confidence: 99%

Optimization of ultrasound-assisted extraction of flavonoid compounds and their pharmaceutical activity from curry leaf (Murraya koenigii L.) using response surface methodology

Ghasemzadeh

Jaafar

Karimi

et al. 2014

BMC Complement Altern Med

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BackgroundExtraction prior to component analysis is the primary step in the recovery and isolation of bioactive phytochemicals from plant materials.MethodsResponse surface methodology was applied to optimize ultrasound-assisted extraction conditions followed by ultra high performance liquid chromatography (UHPLC) to achieve high catechin, myricetin, and quercetin contents, and high antioxidant and anticancer activities in the curry leaf extracts. The antioxidant and anticancer activities of the leaf extracts were determined by the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays, respectively. The central composite experimental design (3-level, 3-factorial) was employed to consider the effects of ultrasonic power (80–150 W), temperature (40–80°C), and methanol dilution (40–80%) on the properties of the curry leaf extracts.ResultsIt was found that ultrasonic power of 145.49 W at 55.9°C with 80% methanol was the most appropriate set of conditions for the extraction of catechin, myricetin, and quercetin from curry leaves with the consequent high antioxidant activity. Using the optimum extraction conditions, the extraction yields of catechin, myricetin, and quercetin were 0.482, 0.517, and 0.394 mg/g DW, respectively, and the antioxidant activity was enhanced to 83%. The optimized extract showed more distinct anticancer activity against HeLa cancer cells in a concentration of 67.2 μg/mL (P < 0.01) without toxicity to normal cells.ConclusionsThe results indicated that the pharmaceutical quality of curry leaves could be improved significantly by optimizing the extraction process using response surface methodology.

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“…Murraya koenigii leaves to be a potent source of proteasome inhibitors that lead to cancer cell death. The active component(s) such as koenidine from the leaf extract might be playing role in the reducing the proliferation rate of the cells [39]. When we tested the KND on human brain cells, the similar kind of effects such as a significant reduction on cell proliferation was observed.…”

Section: Discussionmentioning

confidence: 54%

Mitigative Effects of Koenidine in Reducing the Toxicity Induced by Heavy Metal Lead in Human Neuroblastoma Cells

Challa¹

2016

J Chem Biol Ther

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Lead is a heavy metal been widely used in the manufacturing of glass, pigments, makeup, pipes, wine, cooking, electronic components and batteries. It is a multimedia environmental pollutant, and its exposure via different routes is found to affect metabolic functions. Primarily Lead (Pb) plays an important role in inducing oxidative stress one of the important pathologies and can affect the functioning of antioxidant defense system. The cell growth and proliferation is severely affected with the exposure of lead in designated quantities. There is no specific therapy available to treat the toxicity being generated by Pb and the efforts are on to find suitable natural biologically active components that can effectively combat the toxicity. Koenidine (KND) one of the active ingredients from the plant Murraya koenigii was considered to test its efficacy in mitigating the toxicity elicited by Pb. Human SH-SY5Y neuroblastoma cells were exposed to different concentrations (0.01-10 μM) of Pb for 48 h and the cell viability was determined. The IC50 was observed at 5 μM and this particular concentration used for further investigations. The cells were also pre-treated with KND with a range of concentration from 0-100 μM for 48 h, no change in the cell viability was observed. KND was treated (50 μM) to the cells to verify its protective effects against the pre-treatment of Pb at a concentration of 5 μM 48 h, resulted in the significant increase in the cell viability at least by 29.4% from 12.1% when compared with Pb alone treated group. Intracellular glutathione, caspase-3 and prostaglandin E2 levels were quantified to find the protective effects of koenidine. Some significant results (but less than 18%) were observed in decreasing the oxidative stress, combating apoptosis and lessening the inflammation. This clearly indicates that koenidine can be effectively used to mitigate the toxicity provoked by Pb indicating the nature of koenidine as an effective antioxidant, anti-apoptotic, and anti-inflammatory agent.

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Murraya koenigii leaf extract inhibits proteasome activity and induces cell death in breast cancer cells

Cited by 50 publications

References 37 publications

Apigenin inhibits growth and induces apoptosis in human cholangiocarcinoma cells

Apigenin inhibits growth and induces apoptosis in human cholangiocarcinoma cells

Optimization of ultrasound-assisted extraction of flavonoid compounds and their pharmaceutical activity from curry leaf (Murraya koenigii L.) using response surface methodology

Mitigative Effects of Koenidine in Reducing the Toxicity Induced by Heavy Metal Lead in Human Neuroblastoma Cells

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