Murine thymomas induced by fractionated-X-irradiation have specific T-cell receptor rearrangements and characteristics associated with day-15 to -16 fetal thymocytes.

Amari, N. M. B.; Meruelo, Daniel

doi:10.1128/mcb.7.12.4159

Cited by 15 publications

(4 citation statements)

References 39 publications

(62 reference statements)

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“…Cells. In vitro-adapted tumors (radiation leukemia virusinduced tumors and X-irradiation-induced tumors) of various inbred mouse strains were maintained in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (FBS) (2,19,20 prepared from quiescent and activated splenocytes (A and C) and thymocytes (B) harvested at the indicated times following activation with the Tand B-cell mitogens, ConA (10 p.g/ml) and LPS (50 p.g/ml). After electrophoresis and Northern transfer, ERR transcripts (approximately 9 and 8 kb) were detected by hybridization to 32P-labelled ERR cDNA (BamHI-EcoRI) probe.…”

Section: Methodsmentioning

confidence: 99%

Enhanced gene expression of the murine ecotropic retroviral receptor and its human homolog in proliferating cells

Yoshimoto

Meruelo

1992

J Virol

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The receptor for gp70 envelope glycoprotein of murine ecotropic leukemia virus is essential for virus entry into the host cell and has been recently demonstrated to function as a cationic amino acid transporter. In the experiments reported herein, we compared the gene expression of the murine ecotropic retroviral receptor (ERR) and its human homolog (H13) in rapidly proliferating cells versus resting cells using four different systems. (i) The expression of ERR gene is enhanced during activation of T and B lymphocytes by concanavalin A and lipopolysaccharide, respectively. Similar enhancement is observed by adding phorbol 12-myristate 13-acetate (PMA) or calcium ionophore (A23187). These phenomena appear to involve protein kinase C; two PMA analogs, 4 alpha-phorbol and 4 alpha-PMA, lacking the ability to activate protein kinase C fail to induce elevated levels of gene expression, and the protein kinase C inhibitor, H7 [1-(5-isoquinolinylsulfonyl)-2-methylpiperazine dihydrochloride[, inhibits the enhancement induced by PMA. (ii) Friend murine leukemia virus induces rapid splenomegaly, and acute erythroleukemia in sensitive mice. Concomitantly with splenomegaly, ERR gene expression in spleen cells increases dramatically. (iii) The level of expression of the ERR or H13 gene in a variety of tumor cells is highly elevated compared with the level in noncancerous cells. (iv) H13 gene expression decreases upon terminal differentiation of the human promyelocytic leukemia cell line HL-60 into granulocytes or macrophages by dimethyl sulfoxide or PMA, respectively. These results suggest that ERR and H13 genes play an important role in cellular proliferation.

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Section: Methodsmentioning

confidence: 99%

Enhanced gene expression of the murine ecotropic retroviral receptor and its human homolog in proliferating cells

Yoshimoto

Meruelo

1992

J Virol

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“…Some reports have shown that nearly all phenotypes of lymphoma cells corresponding to each stage of normal differentiation were induced by radiation (Gremers et al 1986) and carcinogens (Diamond et al 1987). Other reports have suggested that the lymphomas could result from the expansion of lymphoma cells arrested at a specific maturation stage (Amari and Meruelo 1987). These results suggests that is a strain difference in the predominant phenotype of lymphoma.…”

Section: Fractionatedmentioning

confidence: 38%

Strain Difference in the Phenotype of Radiation-Induced Thymic Lymphoma in DBA1 and NFS Mice.

1999

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SummaryWe investigated the phenotype of thymic lymphoma induced by fractionated wholebody X-irradiation in DBA1 and NFS mice using mAbs for different cell surface antigens and flow . These results suggests that is a strain difference in the predominant phenotype of lymphoma. To ascertain this, we analyzed cell surface phenotype of radiation-induced lymphomas from DBA1 and NFS mice strain, which are both highly susceptible to induction of lymphoma by fractionated X-irradiation (Mori and Takamori 1989, Sally andFrank 1989). The phenotype of lymphoma cells was determined on the basis of different surface antigens, such as CD4, CD8, CD3, HSA, Thy-1, B220, VLA-4 and LECAM-1. The result demonstrated that there is a clear strain difference in the phenotypes of lymphoma between DBA1 and NFS mice.Materials and methods AnimalsFemale DBA1 and NFS mice were used in this study. DBA1 mice were originally provided by Dr. J. Hayakawa (Kanazawa University). The origin of NFS mice was previously described (Mori and Takamori 1989). Mice have been bred by brother-sister mating in our laboratory.2. X-irradiation X-irradiation was carried out with a Radioflex X-ray generator (Rigaku Denki Co. Ltd., Japan) operated at 250 kV and 12.5 mA with filters of 0.3 mm Cu and 0.5 mm Al. Mice were exposed to 4

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“…In the normal adult thymus less than 3% of the cells in the cortex express MEL-14 hi or IL-2R + [127,128] and the presence of MEL-14 hi may signify appearance of a leukemic clone [129]. Additionally, most of the TL tumors bear T-lymphocyte specific antigens Thy-1, Lyt-1, and Lyt-2 [130,131].…”

Section: Resultsmentioning

confidence: 99%

Mouse Models for Efficacy Testing of Agents against Radiation Carcinogenesis — A Literature Review

Rivina

Schiestl

2012

IJERPH

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As the number of cancer survivors treated with radiation as a part of their therapy regimen is constantly increasing, so is concern about radiation-induced cancers. This increases the need for therapeutic and mitigating agents against secondary neoplasias. Development and efficacy testing of these agents requires not only extensive in vitro assessment, but also a set of reliable animal models of radiation-induced carcinogenesis. The laboratory mouse (Mus musculus) remains one of the best animal model systems for cancer research due to its molecular and physiological similarities to man, small size, ease of breeding in captivity and a fully sequenced genome. This work reviews relevant M. musculus inbred and F1 hybrid animal models and methodologies of induction of radiation-induced leukemia, thymic lymphoma, breast, and lung cancer in these models. Where available, the associated molecular pathologies are also included.

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Murine thymomas induced by fractionated-X-irradiation have specific T-cell receptor rearrangements and characteristics associated with day-15 to -16 fetal thymocytes.

Cited by 15 publications

References 39 publications

Enhanced gene expression of the murine ecotropic retroviral receptor and its human homolog in proliferating cells

Enhanced gene expression of the murine ecotropic retroviral receptor and its human homolog in proliferating cells

Strain Difference in the Phenotype of Radiation-Induced Thymic Lymphoma in DBA1 and NFS Mice.

Mouse Models for Efficacy Testing of Agents against Radiation Carcinogenesis — A Literature Review

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