Murine sarcoma virus pseudotypes acquire a determinant specifying N or B tropism from leukaemia virus during rescue

Bassin, Robert H.; Duran-Troise, Graciela; Gerwin, Brenda I.; Gisselbrecht, S; Rein, Alan

doi:10.1038/256223a0

Cited by 36 publications

(14 citation statements)

References 26 publications

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“…(iii) A single p30 tryptic peptide can indicate whether a virus is N-tropic, B-tropic, xenotropic, or from a wild mouse. Thus, a small, structurally variable region of the MuLV p30 is functionally associated with Fv-1 tropism and could directly or indirectly be the viral target (2,3) of the product(s) of the Fv-l locus of the mouse (4). This supports previouts biochemical and genetic data (5,6) suggesting that p30 is the viral determinant of Fv-1 tropism.…”

supporting

confidence: 74%

Structural markers on core protein p30 of murine leukemia virus: functional correlation with Fv-1 tropism.

Gautsch¹,

Elder²,

Schindler³

et al. 1978

Proc. Natl. Acad. Sci. U.S.A.

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Tryptic peptide maps from more than 50 isolates of murine leukemia virus (MuLV) have shown that, in general, the structure of core protein p30 is highly conserved. However, a structurally variable region of p30 has been identified that is functionally associated with Fv-1 tropism. On the basis of this structural variability, MuLV strains can be classified as B-tropic, N-tropic, xenotropic, and/ MATERIALS AND METHODSThe sources of the MuLV strains utilized in this study are listed in Table 1. Viruses were concentrated and purified from tissue culture media obtained from the third and fourth subcultures by differential centrifugation and banding in 15-50% sucrose gradients, as previously described (11, 13). Virus with a buoyant density of 1.16 g/cm3 was collected and the viral proteins were separated by sodium dodecyl sulfate/polyacrylamide gel electrophoresis (24). Tryptic peptide maps were derived as previously described (1, 13). RESULTSWe have made two-dimensional tryptic peptide maps of the p30 from more than 50 strains of ecotropic, amphotropic, and xenotropic MuLVs isolated from numerous strains of mice. As expected from the NH2-terminal amino acid sequence homology (25), all of the MuLV p3Os displayed a remarkable structural similarity. This indicated that these viruses are all members of a single closely related group, despite their rather wide phenotypic diversity. Fig. 1 upper left is a representative tryptic peptide map of an MuLV p30 from a BALB/c mouse. Twelve peptides have been numbered in Fig. 1 upper right to facilitate subsequent discussion. Some of the lighter spots (e.g., 1, 4, 6, and 9) always appear with a darker spot as a doublet. The spots that appear as doublets (1-2, 4-5, 6-7, 9-10, and 12) could represent the same tyrosine-containing peptide, the darker of the two containing monoiodotyrosine and the lighter one diiodotyrosine (26). As long as labeling and trypsin treatment conditions were held constant, identical peptide maps were generated from the same protein time after time. Several spots or "smudges" were seen in the right half of the MuLV p30 maps. These regions were usually not resolved as discrete spots but showed a similar pattern in most of the maps and were not considered in the comparisons described below.Fine Structural Differences in MuLV p3Os Are Excellent Markers for Specific Viral Strains. Although all MuLV p3Os obviously belong to a single structural group, there are minor differences in the peptide profiles indicative of small variances in the amino acid sequence of these molecules. These differences are reproducibly seen and are thus excellent markers for certain specific MULV strains.

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supporting

confidence: 74%

Structural markers on core protein p30 of murine leukemia virus: functional correlation with Fv-1 tropism.

Gautsch¹,

Elder²,

Schindler³

et al. 1978

Proc. Natl. Acad. Sci. U.S.A.

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“…It has been proposed on the basis of the experiments of Bassin et al (29) and of Rein et al (30) that the Fv-1 effector molecule interacts with a protein structure of the virus. If this protein is the reverse transcriptase molecule, then it is interfering with a yet-to-be-defined effect of the enzyme on circularization or integration of the proviral DNA.…”

Section: Methodsmentioning

confidence: 99%

Host restriction of Friend leukemia virus: synthesis and integration of the provirus.

Sveda¹,

Soeiro²

1976

Proc. Natl. Acad. Sci. U.S.A.

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Host restriction of exogenous infection by murine leukemia viruses is controlled in vitro predominantly by the murine Fv-1 locus. The mechanism of this-host restriction was investigated by comparing the early events in the replication of N-tropic versus B-tropic Friend leukemia virus in NIH 3T3 cells. These cells, which are Fv-l" in type, are permissive for the N-tropic strain, but nonpermissive for the -tropic strain, which replicates permissively in Balb/c cells. We have studied the synthesis, intracellular location, and molecular form of virusspecific DNA early in replication by means of molecular hybridization with a virus-specific DNA probe. Our results suggest that in the permissive infection viral DNA rapidly becomes integrated with cellular DNA. However, in the nonpermissive infection, although almost equal amounts of both positive and negative strand viral DNA are synthesized, integration of the provirus does not occur.

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“…But since neither the blood types nor HL-A types tested correlated with in vitro susceptibility, the evidence is that expression of these antigens is not linked to expression of factors important for in vitro susceptibility. In vitro-grown chick fibroblasts vary in adsorption of C-type virus (Vogt & Ishizaki, 1965) whereas the resistance of mouse fibroblasts to certain C-type viruses resides in intracellular events (Bassin et al, 1975) as does cellular resistance to vesicular stomatitis (Simpson & Obijeski, 1974). Non-permissiveness, of course, could also reflect true interference from pre-existing infection of the cells.…”

Section: Discussionmentioning

confidence: 99%

In vitro susceptibilities of normal human skin fibroblasts to oncoviruses, and the decreased susceptibility to HSV of fibroblasts from untreated Hodgkin's patients

Ebbesen¹,

Vestergaard²,

Ting³

et al. 1981

Br J Cancer

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Summary.-Fibroblast cultures established from the skin of 56 healthy controls and 15 untreated Stages I and II Hodgkin's patients (HD) were studied in their 3rd, 4th and 5th in vitro passage with respect to transformation with Simian sarcoma virus (SSV) and SV40 and with respect to replication of herpes simplex virus (HSV) Types 1 and 2, pox virus and interferon release. Susceptibility to the 5 viruses varied independently, except for an inverse correlation between susceptibility to SSV and HSV. HD cultures showed a depressed replication of both types of HSV. There was a borderline (P=0.02) correlation between magnitude of HSV replication and presence of HL-A type B-w44, but this does not explain the HD control difference. Furthermore, the level of serum antibodies to HSV common antigen was not related to magnitude of in vitro replication. The results thus speak against generally enhanced cellular susceptibility to HSV as a reason for the high titres of serum antibodies to HSV in HD patients.

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Murine sarcoma virus pseudotypes acquire a determinant specifying N or B tropism from leukaemia virus during rescue

Cited by 36 publications

References 26 publications

Structural markers on core protein p30 of murine leukemia virus: functional correlation with Fv-1 tropism.

Structural markers on core protein p30 of murine leukemia virus: functional correlation with Fv-1 tropism.

Host restriction of Friend leukemia virus: synthesis and integration of the provirus.

In vitro susceptibilities of normal human skin fibroblasts to oncoviruses, and the decreased susceptibility to HSV of fibroblasts from untreated Hodgkin's patients

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