Murine RAW 264.7 cell line as an immune target: are we missing something?

Merly, Liza; Smith, Sylvia L.

doi:10.1080/08923973.2017.1282511

Cited by 75 publications

(45 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RAW 264.7 is the murine macrophage cell line used for screening natural products for biological activity and for predicting their potential effect on primary cells or in vivo. The RAW 264.7 cell line response is used to determine the effective biological activity and regarded as a reflection of the potential human de novo response [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Metabolites in White Flowers of Magnolia Denudata Desr. and Violet Flowers of Magnolia Liliiflora Desr.

Park

Lee

et al. 2018

Molecules

View full text Add to dashboard Cite

A total of seven phenolics and 44 metabolites was profiled in white flowers of Magnolia denudata and violet flowers of Magnolia liliiflora using high-performance liquid chromatography (HPLC), electrospray ionization-mass spectrometry (ESI-MS), and gas chromatography time-of-flight mass spectrometry (GC-TOFMS). Seven phenylpropanoid compounds were identified in white flowers by liquid chromatography mass spectrometry (LC-MS). An HPLC analysis showed that phenylpropanoid accumulation in violet flowers was 1.48 times higher than that in white flowers. Furthermore, superoxide dismutase (SOD)-like activity and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity were determined to investigate the antioxidant properties of secondary metabolites in different flowers. Violet flowers showed higher SOD-like and DPPH activity than white flowers. In addition, anti-inflammatory activity measured using a nitric oxide assay was higher in violet flowers than in white flowers. Our results provide valuable information on the relationship between primary and secondary metabolites, and synergistic antioxidant and anti-inflammatory properties derived from phenolic compounds in different colored flowers.

show abstract

Section: Introductionmentioning

confidence: 99%

Analysis of Metabolites in White Flowers of Magnolia Denudata Desr. and Violet Flowers of Magnolia Liliiflora Desr.

Park

Lee

et al. 2018

Molecules

View full text Add to dashboard Cite

show abstract

“…Recent research has found various macrophages roles and functions, and macrophages play an important role in maintaining homeostasis and normal physiological conditions by coordinating a variety of biological activities [ 23 ]. Actually, studies examining the putative immunological properties of natural products and/or their derivatives depend heavily on the use of cell lines as an initial screen for biological activity [ 24 ]. In addition, because of their popularity as targeted single cells for assessing immune reactivity [ 25 – 28 ], we also chose RAW 264.7 macrophages for our current study.…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory and Antioxidant Effects of Carpesium cernuum L. Methanolic Extract in LPS-Stimulated RAW 264.7 Macrophages

Park

Cheon

Lee

et al. 2020

Mediators of Inflammation

View full text Add to dashboard Cite

A hypernomic reaction or an abnormal inflammatory process could cause a series of diseases, such as cardiovascular disease, neurodegeneration, and cancer. Additionally, oxidative stress has been identified to induce severe tissue injury and inflammation. Carpesium cernuum L. (C. cernuum) is a Chinese folk medicine used for its anti-inflammatory, analgesic, and detoxifying properties. However, the underlying molecular mechanism of C. cernuum in inflammatory and oxidative stress conditions remains largely unknown. The aim of this study was to examine the effects of a methanolic extract of C. cernuum (CLME) on lipopolysaccharide- (LPS-) induced RAW 264.7 mouse macrophages and a sepsis mouse model. The data presented in this study indicated that CLME inhibited LPS-induced production of proinflammatory mediators such as nitric oxide (NO) and prostaglandin E2 (PGE2) in RAW 264.7 cells. CLME treatment also reduced reactive oxygen species (ROS) generation and enhanced the expression of heme oxygenase-1 (HO-1) protein in a dose-dependent manner in the LPS-stimulated RAW 264.7 cells. Moreover, CLME treatment abolished the nuclear translocation of nuclear factor-κB (NF-κB), enhanced the activation of nuclear factor-erythroid 2 p45-related factor 2 (Nrf2), and reduced the expression of extracellular signal-related kinase (ERK) and ERK kinase (MEK) phosphorylation in LPS-stimulated RAW 264.7 cells. These outcomes implied that CLME could be a potential antioxidant and anti-inflammatory agent.

show abstract

“…Its cell line is popular mainly in exploratory experiments about putative biological agents or drugs [37]. However, there are some investigations that consider this cell line not so good to evaluate inflammatory response, since it is usually slow to respond to stimulation and often constitutively exhibit high levels of cytokines in the absence of stimulant, including high levels of IL-1β and TNF-α [38]. Despite these limitations we believe that RAW cells could be useful in the in vitro studies considering some important methodological points, such as conducted analysis just in 72 hours cell cultures and considering as basal reference of cytokines and other molecules levels as found in untreated control cells cultured concomitantly to treatments and in the same conditions.…”

Section: Discussionmentioning

confidence: 99%

Antidepressant Drugs Modulate Differentially Anti-inflammatory Lithium’s Property: An in Vitro and in Vivo Study

Barbisan¹,

Prado-Lima²,

Azzolin³

et al. 2018

JPP

View full text Add to dashboard Cite

Li (lithium), a mood stabilizer has anti-inflammatory effect. However, in clinical practice, Li can be administered together with other antidepressants drugs, such as FLX (fluoxetine), IMI (imipramine), NOR (nortriptyline) and ESC (escitalopram). As interaction between Li and these antidepressant drugs on inflammatory modulation has not been investigated yet, we performed an in vitro protocol using a non-human macrophage cell line. Oxidative and inflammatory markers, as well as cell cycle analysis and cytokine gene expressions were compared among treatments. An IR (inflammatory ratio) was calculated based on the following oxidative-inflammatory variables: nitric oxide, superoxide anion, reactive oxygen molecules, cytokines IL-1β, IL-6, TNF-α and IL-10. The in vitro calculated IR data were validated through an in vivo analysis of 154 human subjects with similar IR. Li and control cells presented similar IR values. FLX, NOR and IMI increased slightly IR values indicating some proinflammatory effect, whereas ESC decreased IR values indicating some anti-inflammatory effect. However, cells exposed to Li + ESC triggered a proinflammatory response on macrophages. Thus, IR comparison results suggest that the Li anti-inflammatory effect is not universal and could be influenced by both basal macrophage-inflammatory state and interaction of other psychiatric drugs. These results could be useful to understand some inconsistencies observed in human studies involving Li and other psychiatric drugs.

show abstract

Murine RAW 264.7 cell line as an immune target: are we missing something?

Cited by 75 publications

References 17 publications

Analysis of Metabolites in White Flowers of Magnolia Denudata Desr. and Violet Flowers of Magnolia Liliiflora Desr.

Analysis of Metabolites in White Flowers of Magnolia Denudata Desr. and Violet Flowers of Magnolia Liliiflora Desr.

Anti-Inflammatory and Antioxidant Effects of Carpesium cernuum L. Methanolic Extract in LPS-Stimulated RAW 264.7 Macrophages

Antidepressant Drugs Modulate Differentially Anti-inflammatory Lithium’s Property: An in Vitro and in Vivo Study

Contact Info

Product

Resources

About