Murine lymphoma cells possess blood group Tn-, T-, N-, M- and S-active substances

Gf, Springer; Pr, Desai; Tegtmeyer, H.; Schirmacher,; Cheingsong-Popov, Rachanee

doi:10.1007/bf00365514

Cited by 8 publications

(6 citation statements)

References 12 publications

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“…Treatment of cells with either glycosidases (33), lectins (33), tunicamycin (34,35), or neuraminidase (31) modulates their attachment and spreading in vitro. Recently, cell-surface lectins on normal hepatocytes that aggregate liver-metastasizing tumor cells and that may be involved in the organ selectivity of these cells have been identified (36)(37)(38). These findings support the conclusions of earlier correlative studies that linked the cell-surface protein profiles of different tumor cell sublines with their preferred organ colonization (39,40).…”

Section: Discussionsupporting

confidence: 78%

Oligosaccharide modification by swainsonine treatment inhibits pulmonary colonization by B16-F10 murine melanoma cells.

Humphries¹,

Matsumoto²,

White³

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

165

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Oligosaccharide moieties of cell-surface glycoconijugates are thought to be involved in recognition events associated with tumor metastasis and invasion. Using swainsonine (SW), an inhibitor of Golgi a-mannosidase II that results in the formation of hybrid-type oligosaccharides on N-linked glycoproteins, we have tested the hypothesis that specific glycan structures are required for pulmonary colonization by tumor cells. B16-F10 murine melanoma cells were treated with SW in growth medium and then injected intravenously into syngeneic C57BL/6 mice. This treatment resulted in dramatic inhibition ofcolonization, but it had no effect on B16-F1O viability or on cellular tumorigenicity after subcutaneous implantation. SW-treated radiolabeled B16-F1O cells were cleared from the lungs at a greater rate than control cells, suggesting that one effect of treatment is to alter tumor cell retention in the target organ. Our results implicate specific glycan structures in pulmonary colonization and offer a potential approach for identification of specific macromolecules involved in tumor cell-organ recognition during metastasis. MD), and passaged twice weekly by short exposure to 0.25% trypsin/0.02% EDTA (GIBCO). Only cells <60 days old were used for these studies. Cultures were routinely tested for microbial infection and verified to be free of mycoplasma.Pulmonary Colonization. Six-week-old pathogen-free C57BL/6 mice (Charles River Breeding Laboratories, Wilmington, MA) were quarantined for 1 week and used over an age range of 8-10 weeks. SW was obtained either as a gift from H. P. Broquist (Vanderbilt University, Nashville, TN) or from Boehringer Mannheim and was dissolved in water.B16-F1O cells were plated at 106 or 5 x 105 cells per 75-cm2 flask and 2 or 3 days later, respectively, were treated with SW in growth medium for 18-22 hr. The just confluent cultures were then washed with divalent cation-free Dulbecco's phosphate-buffered saline (PBS-; GIBCO), detached with 0.02% EDTA for 2 min, and resuspended gently to 2.5 x 105 cells per ml in Dulbecco's MEM containing 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (GIBCO).Prior to injection, animals were warmed for 10 min to elicit vasodilation, and 0.2-ml aliquots of each cell suspension (containing 5 X 104 cells) were then injected slowly into the lateral tail vein as described by Fidler (19 The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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Section: Discussionsupporting

confidence: 78%

Oligosaccharide modification by swainsonine treatment inhibits pulmonary colonization by B16-F10 murine melanoma cells.

Humphries¹,

Matsumoto²,

White³

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

165

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“…Thomsen-Friedenreich (TF) antigens have been shown to be expressed in carci noma and lymphoma cells [1][2][3]. In normal cells, these antigens are not expressed or are covered by sialic acid depending on the cell type.…”

Section: Introductionmentioning

confidence: 99%

Increased Expression of Thomsen-Friedenreich Antigens during Tumor Progression in Breast Cancer Patients

Wolf

Ludwig²,

Fritz³

et al. 1988

Tumor Biol

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Paraffin-embedded tissue sections of primary tumors and lymph node metastases of 80 breast cancer patients were tested for the expression of Thomsen-Friedenreich (TF) antigens with the aid of a monoclonal IgM antibody (49H8) highly specific for phenyl-beta-galactoside. TF antigens were not expressed in 16 different normal tissues with the exception of some structures in the kidney. In tumor cells, two types of antigen expression were found; namely, cryptic and exposed. From stage T1/No to stages T2–4/N1,2 the number of cases expressing high amounts of TF antigens increased from 9% (2/22) to 22% (4/18) while the percentage of patients with low intensity of antibody binding was reduced from 59% (13/22) to 39% (7/18). The total amount of TF-positive primary tumors at stages T2–4/No increased from 42% (8/19) to 69% (18/26) when lymph nodes were infiltrated (T2–4/N1,2). At this stage 80% (21/26) of the patients with lymph node infiltration carried TF antigens in the nodes. The distribution of antigens was heterogeneous among the tumor cells and was expressed mainly in an apical or luminal position. The increased expression of antigens was attributed to exposed TF antigens, while cryptic antigens remained constant. When primary tumors expressed exposed TF antigens, the corresponding lymph nodes also contained exposed antigen. The same was true for the cryptic antigen. The data demonstrate an increase in the intensity of TF antigen expression during tumor progression and a spread of TF-positive tumor cells into the axillary lymph nodes with an increasing number of breast cancer patients being TF-positive at this stage.

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“…These polyclonal antibodies, present in all human sera, have been used in studies of the presence of T and Tn EPs in CA [10,21]. Using monospecific polyclonal antisera, Springer et al [11,27] showed in 1983 that murine DBA/2 ESb lymphoma cells have T and Tn specific structures on their surfaces. In 1985, using the polyclonal antisera, Springer et al [lo] showed the presence of T and Tn on various T-lymphoblastic and erythroleukemic cell lines; however, the B-lymphoblastic cell line RPMI-7666 was found to be negative.…”

Section: Discussionmentioning

confidence: 99%

“…Berdinskikh et al [9] suggested that leukocytes from leukemia patients have T antigens. Using polyclonal antisera, Springer et al [10,11] showed the presence of Tn and T on various T-lymphoblastic and erythroleukemic cell lines. Tn expression on red blood cells has also been shown to occur in B-cell lymphoma [ 121 and myelodysplasia [13].…”

Section: Introductionmentioning

confidence: 99%

Flow cytometric analysis of T and Tn epitopes on chronic lymphocytic leukemia cells

et al. 1996

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lmmunophenotyping of peripheral blood lymphocytes from six patients with B-cell chronic lymphocytic leukemia (6-CLL) and five normal volunteers was done and their T and Tn epitopes analyzed using specific monoclonal antibodies and flow cytometry. Lymphocytes from all patients showed strong Tn expression as compared to normal control lympho-cytes. By contrast, T antigen was not expressed. The Tn expression may be a useful diagnostic and prognostic marker for B-CLL.

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Murine lymphoma cells possess blood group Tn-, T-, N-, M- and S-active substances

Cited by 8 publications

References 12 publications

Oligosaccharide modification by swainsonine treatment inhibits pulmonary colonization by B16-F10 murine melanoma cells.

Oligosaccharide modification by swainsonine treatment inhibits pulmonary colonization by B16-F10 murine melanoma cells.

Increased Expression of Thomsen-Friedenreich Antigens during Tumor Progression in Breast Cancer Patients

Flow cytometric analysis of T and Tn epitopes on chronic lymphocytic leukemia cells

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