“…P1, a synthetic peptide derived from the cDNA sequence of 3M-1 (LLRRRHGDRRSTMSAEVP) was manually synthesized by the simultaneous multiple peptide method of Houghten (29,30 T cell lines (M52 and M61) and clones. CD8+ T cell lines and clones were isolated from immunized mice as previously described (26) and propagated by weekly passage with 20 jig/ml antigen (SRTA, P1, or PPD), 20% MLA-144 supernatants as a source or IL-2 and other growth factors (31) (19,20). Induced splenocytes were then depleted with aCD4 Ab (from GK 1.5 hybridoma [33] ) and a mixture of guinea pig and rabbit C. Freezethaw lysates were made of the remaining CD8+ T cells.…”