Murine gammaherpesvirus 68 establishes a latent infection in mouse B lymphocytes in vivo

Sunil-Chandra, N.P.; Efstathiou, Stacey; Nash, Anthony

doi:10.1099/0022-1317-73-12-3275

Cited by 323 publications

(316 citation statements)

References 20 publications

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“…By 30 days p.i., the viral load had decreased by over 1 log but was still almost 1610 4 copies per 100 ng DNA. This pattern of viral DNA detection in the spleens of mice infected as adults was similar to previous observations (Sunil-Chandra et al, 1992b;Tripp et al, 1997;Usherwood et al, 1996). In contrast, following infection of pups, viral load was readily detected in the spleens at 6 days p.i.…”

Section: Chv-68 Pathogenesis Differs At Acute and Latent Stages Follosupporting

confidence: 74%

Infection of neonates with murine gammaherpesvirus 68 results in enhanced viral persistence in lungs and absence of infectious mononucleosis syndrome

Ptaschinski

Rochford

2008

Journal of General Virology

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We used the murine gammaherpesvirus 68 (γHV-68), which serves as a model for human gammaherpesvirus infection, to determine whether age at infection altered the pattern of gammaherpesvirus pathogenesis. We infected mice intranasally at 8 days old (pups) and 6 weeks old (adults) to investigate differences in γHV-68 pathogenesis. There was no difference between adults or pups in acute infection in the lungs at 6 days post-infection (p.i.). However, mice infected as pups exhibited a more disseminated viral infection with viral DNA detected in the spleen, liver and heart as measured by quantitative PCR (Q-PCR). In addition, viral DNA was detected in the lungs of mice infected as pups until 60 days p.i. Three viral transcripts (M2, M3 and M9) were expressed at both 30 and 60 days p.i. In contrast, no viral DNA or mRNA expression was detected in lungs of mice infected as adults at 30 or 60 days p.i. Mice infected as adults experienced a peak in latent infection in the spleen at 16 days p.i., corresponding with an increase in splenic weight and expansion of the Vβ4+ CD8+ T-cell population, similar to infectious mononucleosis observed following infection of young adults with Epstein–Barr virus. However, the increase in splenic weight of infected pups was not as pronounced and no significant increase in Vβ4+ CD8+ T-cell expansion was observed in infected pups. Together, these data suggest that the pathogenesis of murine gammaherpesvirus γHV-68 is age-dependent.

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Section: Chv-68 Pathogenesis Differs At Acute and Latent Stages Follosupporting

confidence: 74%

Infection of neonates with murine gammaherpesvirus 68 results in enhanced viral persistence in lungs and absence of infectious mononucleosis syndrome

Ptaschinski

Rochford

2008

Journal of General Virology

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“…on April 4, 2019. by guest www.bloodjournal.org From infection. Because latent MuHV-4 is predominantly found in B cells in the spleen 31 and macrophages in the peritoneal cavity, 26 the type of cell harboring the latent virus may contribute to these tissue-specific arming effects. Previous authors 12 have identified IL-15/IL-15R signaling as central to the arming of murine NK cells for potent cytotoxicity in vitro via induction of GzmB and perforin protein translation.…”

Section: Latent Herpesvirus Infection Arms Nk Cells 4381mentioning

confidence: 99%

Latent herpesvirus infection arms NK cells

White

Keppel²,

Schneider³

et al. 2010

Blood

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“…by CD8 + T-cells (Ehtisham et al, 1993) but the virus then persists in a latent form in epithelial cells at this site (Stewart et al, 1998). MHV-68 spreads to the spleen during the subsequent viraemia, where it becomes latent in B lymphocytes, macrophages and dendritic cells (Flano et al, 2000;Sunil-Chandra et al, 1992b;Usherwood et al, 1996;Weck et al, 1999).…”

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confidence: 99%

The wood mouse is a natural host for Murid herpesvirus 4

Blasdell

McCracken

Morris

et al. 2003

Journal of General Virology

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Infection of laboratory mice by the Murid herpesvirus 4 (MHV-4) is a much studied model system for gammaherpesvirus pathogenesis. Little, however, is known about its natural host range, epidemiology and pathogenesis outside the laboratory. We have studied MHV-4 infection in freeliving murids in the UK. Using a combination of serology and PCR analysis, we found that MHV-4 was endemic in wood mice (Apodemus sylvaticus) but not in two species of voles (Clethrionomys glareolus, Microtus agrestis). The sites of detection of viral DNA were the lungs and, less commonly, the spleen, emphasizing the importance of the former in virus persistence during natural infection and confirming similar data in laboratory mice.

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Murine gammaherpesvirus 68 establishes a latent infection in mouse B lymphocytes in vivo

Cited by 323 publications

References 20 publications

Infection of neonates with murine gammaherpesvirus 68 results in enhanced viral persistence in lungs and absence of infectious mononucleosis syndrome

Infection of neonates with murine gammaherpesvirus 68 results in enhanced viral persistence in lungs and absence of infectious mononucleosis syndrome

Latent herpesvirus infection arms NK cells

The wood mouse is a natural host for Murid herpesvirus 4

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