Murine fecal proteomics: A model system for the detection of potential biomarkers for colorectal cancer

C, Ang; Rothacker, Julie; Patsiouras, Heather; Burgess, Antony W.; Nice, Edouard C.

doi:10.1016/j.chroma.2009.10.007

Cited by 38 publications

(30 citation statements)

References 94 publications

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“…The murine fecal proteome has previously been examined for identification of possible biomarkers of colorectal adenomas [13]. Ang et al identified 115 proteins of murine origin, which was similar to the number of proteins identified in the present study.…”

Section: Discussionsupporting

confidence: 82%

“…The effect of colitis on the fecal proteome has not yet been analyzed in a controlled system such as a laboratory mouse model. Only a few studies have analyzed the fecal proteome of laboratory mice, but to our knowledge never in the context of IBD [4, 13, 14]. Also, since all Galphai2 -/- mice eventually develop colitis, mice predestined for disease but still in the pre-symptomatic phase can be studied.…”

Section: Introductionmentioning

confidence: 99%

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Elevated fecal peptidase D at onset of colitis in Galphai2-/- mice, a mouse model of IBD

et al. 2017

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BackgroundThe identification of novel fecal biomarkers in inflammatory bowel disease (IBD) is hampered by the complexity of the human fecal proteome. On the other hand, in experimental mouse models there is probably less variation. We investigated the fecal protein content in mice to identify possible biomarkers and pathogenic mechanisms.MethodsFecal samples were collected at onset of inflammation in Galphai2-/- mice, a well-described spontaneous model of chronic colitis, and from healthy littermates. The fecal proteome was analyzed by two-dimensional electrophoresis and quantitative mass spectrometry and results were then validated in a new cohort of mice.ResultsAs a potential top marker of disease, peptidase D was found at a higher ratio in Galphai2-/- mouse feces relative to controls (fold change 27; p = 0.019). Other proteins found to be enriched in Gαi2-/- mice were mainly pancreatic proteases, and proteins from plasma and blood cells. A tendency of increased calprotectin, subunit S100-A8, was also observed (fold change 21; p = 0.058). Proteases are potential activators of inflammation in the gastrointestinal tract through their interaction with the proteinase-activated receptor 2 (PAR2). Accordingly, the level of PAR2 was found to be elevated in both the colon and the pancreas of Galphai2-/- mice at different stages of disease.ConclusionsThese findings identify peptidase D, an ubiquitously expressed intracellular peptidase, as a potential novel marker of colitis. The elevated levels of fecal proteases may be involved in the pathogenesis of colitis and contribute to the clinical phenotype, possibly by activation of intestinal PAR2.

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Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Elevated fecal peptidase D at onset of colitis in Galphai2-/- mice, a mouse model of IBD

et al. 2017

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“…1A). These data confirm the presence of Plg and its derivatives in the healthy human GIT and strengthen previous data reporting the occurrence of plasma proteins in fecal extracts (1,16). The capability of B. animalis subsp.…”

Section: Vol 77 2011supporting

confidence: 81%

Relevance of Bifidobacterium animalis subsp. lactis Plasminogen Binding Activity in the Human Gastrointestinal Microenvironment

Candela

Turroni

Centanni

et al. 2011

Appl Environ Microbiol

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Human plasmin(ogen) is regarded as a component of the molecular cross talk between the probiotic species Bifidobacterium animalis subsp. lactis and the human host. However, up to now, only in vitro studies have been reported. Here, we demonstrate that the probiotic strain B. animalis subsp. lactis BI07 is capable of recruiting plasmin(ogen) present at physiological concentrations in crude extracts from human feces. Our results provide evidence that supports the significance of the B. lactis-plasmin(ogen) interaction in the human gastrointestinal tract.

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“…Interestingly, the concentrations of HPX in OC ascites were higher than normal concentrations in human plasma and serum [52]. Furthermore, HPX was overexpressed during other malignancies, like breast cancer [53] or colorectal cancer [54]. …”

Section: Discussionmentioning

confidence: 99%

Understanding Ovarian Cancer: iTRAQ-Based Proteomics for Biomarker Discovery

Światły

Horała

Matysiak

et al. 2018

IJMS

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Despite many years of studies, ovarian cancer remains one of the top ten cancers worldwide. Its high mortality rate is mainly due to lack of sufficient diagnostic methods. For this reason, our research focused on the identification of blood markers whose appearance would precede the clinical manifestation of the disease. ITRAQ-tagging (isobaric Tags for Relative and Absolute Quantification) coupled with mass spectrometry technology was applied. Three groups of samples derived from patients with: ovarian cancer, benign ovarian tumor, and healthy controls, were examined. Mass spectrometry analysis allowed for highlighting the dysregulation of several proteins associated with ovarian cancer. Further validation of the obtained results indicated that five proteins (Serotransferrin, Amyloid A1, Hemopexin, C-reactive protein, Albumin) were differentially expressed in ovarian cancer group. Interestingly, the addition of Albumin, Serotransferrin, and Amyloid A1 to CA125 (cancer antigen 125) and HE4 (human epididymis protein4) improved the diagnostic performance of the model discriminating between benign and malignant tumors. Identified proteins shed light on the molecular signaling pathways that are associated with ovarian cancer development and should be further investigated in future studies. Our findings indicate five proteins with a strong potential to use in a multimarker test for screening and detection of ovarian cancer.

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Murine fecal proteomics: A model system for the detection of potential biomarkers for colorectal cancer

Cited by 38 publications

References 94 publications

Elevated fecal peptidase D at onset of colitis in Galphai2-/- mice, a mouse model of IBD

Elevated fecal peptidase D at onset of colitis in Galphai2-/- mice, a mouse model of IBD

Relevance of Bifidobacterium animalis subsp. lactis Plasminogen Binding Activity in the Human Gastrointestinal Microenvironment

Understanding Ovarian Cancer: iTRAQ-Based Proteomics for Biomarker Discovery

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