Murine epidermal lipoxygenase (Aloxe) encodes a 12‐lipoxygenase isoform¹

Constitutive expression of 8-lipoxygenase in papillomas and clastogenic effects of lipoxygenase-derived arachidonic acid metabolites in keratinocytes

“…The recombinant enzyme exhibited the same stereospecificity as the native enzyme from phorbol ester-treated mouse epidermis. 15-HETE and 13-HODE generated in cytosol from both transfected and untransfected cells were found to be racemic, as previously reported [26].…”

“…Prostaglandin synthase-1 [31] and at least four lipoxygenases (5-LOX [32], epidermis-type 12-LOX [26], 15-LOX-2 [27], and a novel epidermis-type LOX-2 [20]) have been found to be constitutively expressed in this tissue. In contrast, prostaglandin synthase-2 [31], platelet-type 12-LOX [16], and 8-LOX [19,20] are barely expressed in normal epidermis but are induced in response to skin damage or chemical irritation.…”

cDNA Cloning, Genomic Structure, and Chromosomal Localization of a Novel Murine Epidermis-type Lipoxygenase

“…Ten of 21 clones with correctly sized inserts corresponded to epidermis-type 12(S)-lipoxygenase, 3 to leukocyte-type 12(S)-lipoxygenase, and 4 to 12(R)-lipoxygenase. In addition, 3 clones were found to contain identical inserts representing a novel lipoxygenase, termed epidermis-type lipoxygenase-3 (e-LOX-3), 2 referring to epidermis-type 12(S)-lipoxygenase Kinzig et al, 1997;van Dijk et al, 1995) and 12(R)-lipoxygenase (Krieg et al, 1998) as e-LOX-1 and e-LOX-2, respectively. A 3Ј-RACE strategy using sequence information from the partial Aloxe3 cDNA clone enabled the isolation of the 3Ј-end.…”

Lipoxygenase pathways in Homo neanderthalensis: functional comparison with Homo sapiens isoforms

“…ALOXE3 was fi rst discovered in mouse and human skin ( 56,57 ), and alternative transcripts have been identifi ed. For initial comparison ( Fig.…”