Municipal Wastewaters Carry Important Carbapenemase Genes Independent of Hospital Input and Can Mirror Clinical Resistance Patterns

Teban-Man, Adela; Szekeres, Edina; Fang, Peiju; Klümper, Uli; Hegedüs, Adriana; Baricz, Andreea; Berendonk, Thomas U.; Pârvu, Marcel; Coman, Cristian

doi:10.1128/spectrum.02711-21

Cited by 8 publications

(5 citation statements)

References 76 publications

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“…On the other hand, no isolates carried KPC, although its high dominance in clinical isolates of Klebsiella pneumoniae had been reported in China [ 33 , 34 , 35 ]. The fewer incidences of KPC detection could be the result of the characteristics of environmental samples [ 31 ], the expected high removal of KPC in wastewater treatment [ 12 ], and the small population of KPC in clinical isolates [ 26 , 29 , 36 ] although the reason may be complex.…”

Section: Discussionmentioning

confidence: 99%

“…During quantitative monitoring, which is essential for WBE, the genes that encode carbapenemases are usually quantification targets because of their ubiquitous presence [ 12 , 13 ]. They are carried by various species under Enterobacterales such as Klebsiella quasipneumoniae , Escherichia coli , and Enterobacter cloacae, which were taken from municipal wastewater samples [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

“…They are carried by various species under Enterobacterales such as Klebsiella quasipneumoniae , Escherichia coli , and Enterobacter cloacae, which were taken from municipal wastewater samples [ 14 ]. Molecular-based techniques such as new generation sequencing and real-time polymerase chain reaction (PCR) tests have been used for the quantitative monitoring of carbapenemase genes [ 12 , 15 ]. Carbapenemases constitute a large variety of enzymes that are categorized into three of the four Ambler classes (A, B and D) [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Monitoring Carbapenem-Resistant Enterobacterales in the Environment to Assess the Spread in the Community

2022

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The usefulness of wastewater-based epidemiology (WBE) was proven during the COVID-19 pandemic, and the role of environmental monitoring of emerging infectious diseases has been recognized. In this study, the prevalence of carbapenem-resistant Enterobacterales (CRE) in Japanese environmental samples was measured in the context of applying WBE to CRE. A total of 247 carbapenem-resistant isolates were obtained from wastewater, treated wastewater, and river water. Treated wastewater was shown to be an efficient target for monitoring CRE. The results of the isolate analysis showed that WBE may be applicable to Escherichia coli-carrying New Delhi metallo-β-lactamase (NDM)-type carbapenemase, the Enterobacter cloacae complex and Klebsiella pneumoniae complex-carrying IMP-type carbapenemase. In addition, a certain number of CRE isolated in this study carried Guiana extended spectrum (GES)-type carbapenemase although their clinical importance was unclear. Only a few isolates of Klebsiella aerogenes were obtained from environmental samples in spite of their frequent detection in clinical isolates. Neither the KPC-type, the oxacillinase (OXA)-type nor the VIM-type of carbapenemase was detected in the CRE, which reflected a low regional prevalence. These results indicated the expectation and the limitation of applying WBE to CRE.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Monitoring Carbapenem-Resistant Enterobacterales in the Environment to Assess the Spread in the Community

2022

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“…Leachate was collected from soil at depths of 10 cm, 20 cm, and 30 cm, as well as runoff water at a depth of 40 cm. The samples for Cd and Pb analysis were digested using the nitric acid-peroxide method and measured using a Graphite Furnace Atomic Absorption Spectrometer [25].…”

Section: Determination Of CD and Pb Content In Soil Flowmentioning

confidence: 99%

Remediation of Cadmium and Lead in Mine Soil by Ameliorants and Its Impact on Maize (Zea mays L.) Cultivation

Chen,

Wang,

et al. 2024

Agronomy

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The soil in a lead–zinc mining area, contaminated with heavy metals like cadmium (Cd) and lead (Pb), poses a risk to crops such as maize. Experiments using biochar and sepiolite as soil ameliorants in potted maize showed these substances can mitigate heavy metal contamination. Biochar increased potassium and phosphorus in the soil and maize, while sepiolite significantly boosted overground phosphorus by 73.2%. Both ameliorants transformed Cd and Pb into a more stable state in the soil, reducing their accumulation in maize, especially with biochar, which effectively inhibited metal migration during leaching events. This study provided insights for further improvement of soil amendments and multi-factor application experiments.

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“…Analyses of wastewater containing bacteria mainly of human origin could be helpful for better understanding the community prevalence of antimicrobial-resistant bacteria, AMR genes, and associated MGEs ( 11 ). Recent studies revealed the presence of carbapenemase-encoding genes, such as bla KPC , bla NDM , bla IMP , and bla OXA-48 in wastewater influent in different countries, and the concordance of their prevalence in wastewater and human isolates was generally demonstrated ( 12 – 14 ). Meanwhile, the predominance of bla GES carbapenemase genes mainly harbored by Aeromonas , Enterobacter , and Kluyvera spp.…”

Section: Introductionmentioning

confidence: 99%

Municipal wastewater monitoring revealed the predominance of bla _GES genes with diverse variants among carbapenemase-producing organisms: high occurrence and persistence of Aeromonas caviae harboring the new bla _GES variant bla _GES-48

Tanabe,

Sugawara,

Denda

et al. 2023

Microbiol Spectr

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We characterized carbapenemase-producing organism (CPO) detected in municipal wastewater to better understand the epidemiology of CPOs in the community. In total, 36 samples were collected at six sampling sites every other month from December 2020 to October 2021. CPOs were not recovered from influent taken from inlet A receiving separated sewer line, treated effluents, and river water samples upstream and downstream of the effluent outlet. By contrast, 75 CPOs were detected in all influent samples taken from inlets B and C receiving combined sewer lines collecting both domestic/industrial wastewater and rainwater runoff. Aeromonas caviae was the dominant species (25/75, 33.3%), and the other 11 Aeromonas spp. together accounted for 48% of CPOs. The remaining 39 Enterobacterales strains mainly comprised 17 Klebsiella spp. and 10 Raoultella spp. CPOs carrying bla GES carbapenemase genes were overwhelmingly dominant, accounting for 72 of 75 isolates, including two isolates harboring both bla GES-24 and bla IMP-1 (96%), followed by three bla IMPs -positive isolates, where those carbapenemase genes were mainly carried in diverse class 1 integrons. Among bla GES variants, including six new variants ( bla GES-47 , bla GES-48 , bla GES-49 , bla GES-50 , bla GES-51 , and bla GES-54 ), bla GES-5 was detected in 28 CPOs, with Aeromonas spp. accounting for 53.6% of these organisms. Quantitative analysis revealed that the repeated detection of bla GES-48 -positive A. caviae ST1056 from both inlets B and C ranked the total number of this bacterial clone highest in the wastewater influent. In summary, our study revealed the high prevalence and persistence of diverse bla GES carbapenemase genes among CPOs isolated from influent inlets connected to combined sewer systems. IMPORTANCE The emergence and spread of carbapenemase-producing organisms (CPOs) represent a global health threat because they are associated with limited treatment options and poor clinical outcomes. Wastewater is considered a hotspot for the evolution and dissemination of antimicrobial resistance. Thus, analyses of municipal wastewater are critical for understanding the circulation of these CPOs and carbapenemase genes in local communities, which remains scarcely known in Japan. This study resulted in several key observations: (i) the vast majority of bla GES genes, including six new bla GES variants, and less frequent bla IMP genes were carbapenemase genes encountered exclusively in wastewater influent; (ii) the most dominant CPO species were Aeromonas spp., in which a remarkable diversity of new sequence types was observed; and (iii) CPOs were detected from combined sewer wastewater, but not from separate sewer wastewater, suggesting that the load of CPOs from unrecognized environmental sources could greatly contribute to their detection in influent wastewater.

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Municipal Wastewaters Carry Important Carbapenemase Genes Independent of Hospital Input and Can Mirror Clinical Resistance Patterns

Abstract: Wastewater-based epidemiology has recently been recognized as a valuable, cost-effective tool for antimicrobial resistance surveillance. It can help gain insights into the characteristics and distribution of antibiotic resistance elements at a local, national, and even global scale.

Cited by 8 publications

References 76 publications

Monitoring Carbapenem-Resistant Enterobacterales in the Environment to Assess the Spread in the Community

Monitoring Carbapenem-Resistant Enterobacterales in the Environment to Assess the Spread in the Community

Remediation of Cadmium and Lead in Mine Soil by Ameliorants and Its Impact on Maize (Zea mays L.) Cultivation

Municipal wastewater monitoring revealed the predominance of bla _GES genes with diverse variants among carbapenemase-producing organisms: high occurrence and persistence of Aeromonas caviae harboring the new bla _GES variant bla _GES-48

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Municipal Wastewaters Carry Important Carbapenemase Genes Independent of Hospital Input and Can Mirror Clinical Resistance Patterns

Abstract: Wastewater-based epidemiology has recently been recognized as a valuable, cost-effective tool for antimicrobial resistance surveillance. It can help gain insights into the characteristics and distribution of antibiotic resistance elements at a local, national, and even global scale.

Cited by 8 publications

References 76 publications

Monitoring Carbapenem-Resistant Enterobacterales in the Environment to Assess the Spread in the Community

Monitoring Carbapenem-Resistant Enterobacterales in the Environment to Assess the Spread in the Community

Remediation of Cadmium and Lead in Mine Soil by Ameliorants and Its Impact on Maize (Zea mays L.) Cultivation

Municipal wastewater monitoring revealed the predominance of bla GES genes with diverse variants among carbapenemase-producing organisms: high occurrence and persistence of Aeromonas caviae harboring the new bla GES variant bla GES-48

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Municipal wastewater monitoring revealed the predominance of bla _GES genes with diverse variants among carbapenemase-producing organisms: high occurrence and persistence of Aeromonas caviae harboring the new bla _GES variant bla _GES-48