“…Three 5-minute washes with PBS were performed between steps. Primary antibodies used were: rabbit anti-Liprin-α3 (A232, 1:500; homemade, knockout-verified for STED 20 ), rabbit anti-RIM (A58, 1:500, RRID: AB_887774, knockout-verified for STED 34,61 ), rabbit anti-CaV2.1 (A46, 1:500; RRID: AB_2619841, knockout-verified for STED 50 ), rabbit anti-Munc13-1 (A72, 1:500; RRID: AB_887733, knockout-verified for STED 63 ), rabbit anti-RIM-BP2 (A126, 1:500; RRID: AB_2619739), mouse anti-HA (A12, 1:500; RRID: AB_2565006), mouse anti-Synaptophysin (A100, 1:500; RRID: AB_887824), mouse anti-PSD-95 (A152, 1:500; RRID: AB_10698024), and mouse anti-Gephyrin (A8, 1:500; RRID: AB_2232546). Secondary antibodies used were: goat anti-rabbit Alexa Fluor 488 (S5; 1:500, RRID: AB_2576217), goat anti-mouse IgG1 Alexa Fluor 488 (S7; 1:500, RRID: AB_2535764), goat anti-mouse IgG1 Alexa Fluor 555 (S19, 1:500, RRID: AB_2535769), goat anti-mouse IgG2a Alexa Fluor 633 (S30, 1:500, RRID: AB_1500826), goat anti-guinea pig Alexa Fluor 405 (S51, 1:500, RRID: AB_2827755).…”