Multivitamin and Mineral Supplementation Containing Phytonutrients Scavenges Reactive Oxygen Species in Healthy Subjects: A Randomized, Double-Blinded, Placebo-Controlled Trial

Abstract

Phytonutrients and vitamin and mineral supplementation have been reported to provide increased antioxidant capacity in humans; however, there is still controversy. In the current clinical trial, we examined the antioxidant and DNA protection capacity of a plant-based, multi-vitamin/mineral, and phytonutrient (PMP) supplementation in healthy adults who were habitually low in the consumption of fruits and vegetables. This study was an eight-week, double-blind, randomized, parallel-arm, and placebo-controlled trial. PMP supplementation for eight weeks reduced reactive oxygen species (ROS) and prevented DNA damage without altering endogenous antioxidant system. Plasma vitamins and phytonutrients were significantly correlated with ROS scavenging and DNA damage. In addition, gene expression analysis in PBMC showed subtle changes in superoxide metabolic processes. In this study, we showed that supplementation with a PMP significantly improved ROS scavenging activity and prevented DNA damage. However, additional research is still needed to further identify mechanisms of actions and the role of circulating phytonutrient metabolites.

Meta-Analysis of Randomized Clinical Trials Evaluating Effectiveness of a Multivitamin Supplementation against Oxidative Stress in Healthy Subjects

“…However, we included an 817 per-protocol (PP) population for the meta-analysis after removing 69 samples with incomplete data. All participants were healthy adults with habitually low fruit and vegetable intake, as identified by the recommended food score (RFS) [ 14 , 15 , 16 , 21 ]. The mean age was 38.7 ± 12.6 years and 37.3% ( n = 305) of the sample was male.…”

“…Therefore, the current study aimed to perform a meta-analysis based on the previously performed RCTs of PMS to shed light on the controversy and reach a general conclusion about its effectiveness in maintaining health by reducing oxidative stress. In detail, we collected individual subject datasets of six (three published [ 14 , 15 , 16 ] and three unpublished) RCTs incorporating 886 participants and aggregated the effect sizes. As variables of interest, we selected 19 biological marker variables that are overlapped in at least two studies and applied meta-analysis, considering the heterogeneity of effects from PMS supplementation.…”

Perilla frutescens Extracts Enhance DNA Repair Response in UVB Damaged HaCaT Cells

“…Comet assays were used to assess DNA damage levels in cells [ 17 ]. HaCaT cells were treated with Perilla leaf or callus extracts for 12 h, exposed to UVB, and cultured for 3 or 12 h. Cells were then trypsinized, mixed with low melting agarose gel (LMA), and dispersed onto precoated slides with normal melting agarose (NMA).…”

Impact of Septoplasty on Oxidative DNA Damage Levels in Patients with Marked Nasal Septal Deviation

“…Lipid peroxidation induced by ROS concludes in many unsteady, disintegrated mixtures, with the most significant one being malondialdehyde (MDA), an adequate declarative of lipid peroxidation [6]. But then, ROS are intimately conjoined with DNA rupture and infrastructure alteration [7]. For this intention, the utilisation of any marker of DNA oxidation, such as 8-hydroxy-2 -deoxyguanosine , to measure oxidative stress is of implication [8].…”