Multivisceral transplantation in pigs: a model for research and training

David, André Ibrahim; Chida, Valéria Vieira; Lee, Andre Dong Won; Fiuza, Felipe; Calil, Daniela Medeiros; Maranhão, Eduardo Rullo; Campi, Gabriel Beligni; Llanos, Juan Carlos; Neto, Ben-Hur Ferraz

doi:10.1590/s1679-45082011ao1956

Cited by 2 publications

(4 citation statements)

References 19 publications

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“…To our knowledge, no studies have been conducted to investigate the immunologic outcomes after combining AW‐VCA and ITx in animal models. Due to their anatomical and physiological similarities to humans, the porcine model of ITx can provide a better understanding of graft immunology and physiology and can be performed orthotopically or heterotopically 5–10 . In general, the selection of a specific technique is based on considerations involving technical feasibility, personal experience, reported complication rates, and the design and objectives of the study 6 .…”

Section: Introductionmentioning

confidence: 99%

“…Due to their anatomical and physiological similarities to humans, the porcine model of ITx can provide a better understanding of graft immunology and physiology and can be performed orthotopically or heterotopically. [5][6][7][8][9][10] In general, the selection of a specific technique is based on considerations involving technical feasibility, personal experience, reported complication rates, and the design and objectives of the study. 6 Orthotopic ITx involves grafting in gastrointestinal continuity, which might cause diarrhea, dehydration and electrolyte imbalances, having a significant impact on the physiological state of the pig and interfering with observations of graft immunology.…”

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confidence: 99%

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Heterotopic intestine and abdominal wall transplantation in a miniature swine: A model for graft immunology

Kuo

Chen

Yeh

et al. 2023

The Kaohsiung J of Med Scie

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In order to accurately monitor graft immunology, we have developed a method for performing intestine and abdominal wall transplantation heterotopically in miniature swine. The procedure consisted of simultaneous segmental terminal ileum and full‐thickness abdominal wall transplantation in Lanyu miniature swine, with the intestinal and the abdominal wall grafts being placed on the recipient's bilateral rear flank. Five transplantations were technically successful. One animal died on the first post‐transplant day due to anesthesia‐related issues, three abdominal wall and four intestinal grafts survived, while one abdominal wall graft failed due to vascular thrombosis. Acute cellular rejection (ACR) of the intestinal graft could occur preceding, simultaneously with or following ACR of the abdominal wall graft. Our experimental model demonstrates the technical feasibility of heterotopic intestine and abdominal wall transplantation in miniature swine without grafting in gastrointestinal continuity. This model could be suitable for further studies of graft immunology.

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Section: Introductionmentioning

confidence: 99%

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confidence: 99%

Heterotopic intestine and abdominal wall transplantation in a miniature swine: A model for graft immunology

Kuo

Chen

Yeh

et al. 2023

The Kaohsiung J of Med Scie

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“…The training of anesthetic-surgical teams through experimental surgeries in animals has been considered a fundamental step before its clinical application because of the technical complexity involved in liver transplantation 5,6 . Haschek et al 6 complemented that a pig is an useful model for studies in biomedical research, once it presents similarities to humans in structure and functions 7,8 .…”

Section: Introductionmentioning

confidence: 99%

“…Haschek et al 6 complemented that a pig is an useful model for studies in biomedical research, once it presents similarities to humans in structure and functions 7,8 .…”

Section: Introductionmentioning

confidence: 99%

Immediate graft histological assay, post pig's liver transplantation

Brito

Araújo²,

Cunha³

et al. 2013

Acta Cir. Bras.

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PURPOSE:To describe the vascular and tissue histopathological changes in seven sequential experimental liver transplantations in pigs. METHODS:Fourteen female pigs, Sus domesticus species, with body mass between 5 and 8 kg were utilized. After the end of all anastomoses of the graft implantation in the receptor, the animal was monitored for 30 minutes, and at its end one of the biopsies was collected for histological analysis. The histological criteria utilized were: lytic hepatocyte necrosis, density of septal and portal inflammatory infiltrated, sinusoidal congestion and hemorrhage. The analysis was performed separately for the portal region in zone 1, 2 and 3. RESULTS:Among the structural changes undergone by the graft, those with greater frequency and intensity were vascular congestion and steatosis, which stood out in transplantations 5, 6 and 7. CONCLUSIONS:The technique demonstrated vascular alterations represented by vasocongestion, edema and minimum inflammatory reaction. In relation to the parenchyma, was observed macrovacuolar pan-acinar steatosis, focal lytic and occasional hemorrhages, beyond the accumulation of hemosiderin in Kuppfer's cells.

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Multivisceral transplantation in pigs: a model for research and training

Cited by 2 publications

References 19 publications

Heterotopic intestine and abdominal wall transplantation in a miniature swine: A model for graft immunology

Heterotopic intestine and abdominal wall transplantation in a miniature swine: A model for graft immunology

Immediate graft histological assay, post pig's liver transplantation

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