Multivariate Statistical Approach for Nephrines in Women with Obesity

Abstract

Catecholamines are physiological regulators of carbohydrate and lipid metabolism during stress, but their chronic influence on metabolic changes in obese patients is still not clarified. The present study aimed to establish the associations between the catecholamine metabolites and metabolic syndrome (MS) components in obese women as well as to reveal the possible hidden subgroups of patients through hierarchical cluster analysis and principal component analysis. The 24-h urine excretion of metanephrine and normetanephrine was investigated in 150 obese women (54 non diabetic without MS, 70 non-diabetic with MS and 26 with type 2 diabetes). The interrelations between carbohydrate disturbances, metabolic syndrome components and stress response hormones were studied. Exploratory data analysis was used to determine different patterns of similarities among the patients. Normetanephrine concentrations were significantly increased in postmenopausal patients and in women with morbid obesity, type 2 diabetes, and hypertension but not with prediabetes. Both metanephrine and normetanephrine levels were positively associated with glucose concentrations one hour after glucose load irrespectively of the insulin levels. The exploratory data analysis showed different risk subgroups among the investigated obese women. The development of predictive tools that include not only traditional metabolic risk factors, but also markers of stress response systems might help for specific risk estimation in obesity patients.

Metanephrine and Normetanephrine Urine Excretion in Patients with PCOS

“…Twenty-four-hour urine samples for determining concentrations of metanephrine and normetanephrine were collected from patients and controls during the late follicular phase after specific instructions about the necessary diet. Twenty-fourhour urinary metanephrines were measured by RIA with the following specific characteristics: for metanephrine (MN): reference range < 350 µg/24 h; analytical sensitivity 8 ng/mL, cross-reactivity < 0.02%; for normetanephrine (NMN) reference range < 600 µg/24 h; analytical sensitivity 22 ng/mL, cross-reactivity < 0.08% as in previous studies [15].…”