2019
DOI: 10.1080/10739149.2019.1662438
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Multivariate optimization of ultrasound-assisted extraction for the determination of phenolic compounds in plums (Prunus salicina Lindl.) by high-performance liquid chromatography (HPLC)

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Cited by 14 publications
(8 citation statements)
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“…Phenolic compounds were extracted as described in our previous work by Serradilla et al [ 29 ]. Briefly, 10 g of homogenate was mixed with 50 mL of methanol (0.1% HCl), manually shaken and stored at −20 °C for 24 h. Separation and quantification were performed by HPLC-DAD/FLD, as described by Manzano et al [ 30 ], using a Series 1100 chromatograph (Agilent) equipped with a degasser, a quaternary pump, a Gemini column (NX-C18, 150 × 4.6 mm, 3 µm) thermostated at 40 °C. Phenolic compounds were calculated using calibration curves that were made with standards and following the method of external standards.…”
Section: Methodsmentioning
confidence: 99%
“…Phenolic compounds were extracted as described in our previous work by Serradilla et al [ 29 ]. Briefly, 10 g of homogenate was mixed with 50 mL of methanol (0.1% HCl), manually shaken and stored at −20 °C for 24 h. Separation and quantification were performed by HPLC-DAD/FLD, as described by Manzano et al [ 30 ], using a Series 1100 chromatograph (Agilent) equipped with a degasser, a quaternary pump, a Gemini column (NX-C18, 150 × 4.6 mm, 3 µm) thermostated at 40 °C. Phenolic compounds were calculated using calibration curves that were made with standards and following the method of external standards.…”
Section: Methodsmentioning
confidence: 99%
“…The common structural characteristics all phenolics share are aromatic rings, hydroxyl groups and, commonly, electron double bonds. Phenolics have been extracted using ethanol, methanol, water, ethyl acetate, and dichloromethane by the use of simple solvent extraction under reflux, Soxhlet extraction, assisted ultrasound extraction, and assisted microwave extraction [ 37 , 38 , 39 , 40 , 41 ]. Different extraction methods and solvents have been shown to target the extraction of different phenolics [ 41 , 42 ].…”
Section: Bioactive Components Including Primary Metabolites Phenolics and Their Antioxidant Propertiesmentioning
confidence: 99%
“…Frozen fruits were pitted and homogenised using an Omni Mixer homogeniser (Omni International, Marietta, GA, USA), and 2 g were used to determine phenolic compounds and another 2 g to determine total antioxidant activity (TAA). Phenolic compounds analysis was performed as described in a previous work 16 . Results were expressed as g kg −1 FW.…”
Section: Methodsmentioning
confidence: 99%
“…Phenolic compounds analysis was performed as described in a previous work. 16 Results were expressed as g kg −1 FW. TAA was measured using the DPPH method, and extraction was performed according to Pérez-Jimenez et al, 17 although fresh fruit homogenised was used instead of freeze-dried fruit.…”
Section: Bioactive Compoundsmentioning
confidence: 99%