Multivariate curve resolution of spectral data for the pH-dependent reduction of ferrylmyoglobin by cysteine

Reduction of ferrylmyoglobin by cysteine as affected by pH

“…The rst-order rate constants were obtained by non-linear regression (Matlab R2012a, Mathworks, Natick MA, US). Concentration/time proles were modelled as previously reported 9 in agreement with different reactions scheme using Levenberg-Marquardt algorithm in an optimized toolbox at Matlab R2012a.…”

“…Based on the extracted component spectra and the time concentration proles of the absorbing components in the reactions mixture several reactions mechanisms were suggested. 9 Accordingly, a more focused kinetic study has been initiated in order to identify reaction pathways under different pH-conditions and to determine rate constants for the dominating reactions for discussion of reaction mechanisms behind protection against hypervalent heme pigments by cysteine and cysteine derivatives and homologues.…”

“…Myoglobin was puried according to procedures previously described. 9,11 Acetic acid was from AppliChem GmbH (Darmstadt, Germany). K 2 HPO 4 and KH 2 PO 4 were of analytical grade and supplied by J. T. Baker (Phillipsburg, NJ, US).…”

Restoring important process information from complex optical spectra with MCR-ALS: Case study of actinide reduction in spent nuclear fuel reprocessing

“…and to improve the signal-to-noise ratio. Further on, data analysis was performed applying multivariate curve resolutionalternating least squares (MCR-ALS) [18][19][20] to recover the concentration profiles of actinides during their reduction, and their corresponding spectra. Due to the overall A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT 5 complexity of the process and the media investigated, and despite meticulous data preprocessing, some spectral variations such as shifts and bandwidth changes remained observable throughout the process.…”

Cinética e mecanismo de redução de espécies de ferro-heme hipervalentes pelo H2S, cisteína e CO em relação à proteção do trato gastrointestinal e a qualidade da carne

“…Produção de CO 2 das misturas reacionais mostradas na Figura 21 através da integração do sinal de CO 2 de 2280 à 2400 cm -1 .A velocidade de reação da espécie ROO • com CO tem sido reportada como mais rápida com relação à espécie TrpO • por um fator de 10 2 . 89 dado experimental obtido para o decaimento da espécie •MbFe(IV)=O na presença de CO torna possível (considerando a concentração de solução saturada de CO de 0,7 mmol L -1 ) estimar a constante de velocidade da reação entre Os mecanismos aqui delineados foram propostos com base nas componentes espectrais obtidos através do uso da técnica de MCR e do perfil de concentração com o tempo dos componentes individuais na mistura reacional como proposto anteriormente por Pindstrup et al60 Desta forma, o estudo cinético foi conduzido a fim de identificar-se os diferentes mecanismos de reação variando-se as condições…”