Multivariate correlation of infrared fingerprints and molecular weight distributions with bioactivity of poultry by-product protein hydrolysates

Low Molecular Weight Peptide Fraction from Poultry Byproduct Hydrolysate Features Dual ACE-1 and DPP4 Inhibition

“…Processing conditions (i.e., enzyme choice, enzyme concentration, and hydrolysis time) for production of the crude hydrolysate were based on our previous screening study. 29 Proximate analysis revealed that the resulting crude hydrolysate contained 73.8% protein, while moisture and the remaining ash accounted for 5.7 and 5% of the dry weight, respectively (Table S1). It should be noted that the enzyme preparation constitutes 35−45% (w/w) glycerin (per manu-facture's product description for FoodPro PNL).…”

“…The chosen enzyme and time of hydrolysis were of a hydrolysate with the highest ACE-1 inhibitory activity. The hydrolysis of MDCR was performed as previously outlined in Sorokina et al 29 Briefly, the homogenized MDCR (500 ± 2 g) was suspended in 1 L of Milli-Q water, preheated to 60 °C (±1 °C) and hydrolyzed for 45 min using protease FoodPro PNL (5% relative to the weight of raw material) at 60 °C (±1 °C) and 300 rpm. After 45 min of hydrolysis, the enzyme was thermally inactivated in a water bath at 90 °C (±1 °C) for 15 min.…”

“…SEC of the crude hydrolysate, 3 kDa retentate, 3 kDa permeate, and SEC fractions (SEC method with formic acid in the mobile phase) was performed to study their molecular weight distributions. SEC was performed as described previously 29 with TFA in the mobile phase. The crude hydrolysate and its fractions after the first and second rounds of fractionation were prepared in Milli-Q water at a concentration of 10 mg/mL.…”

“…Mechanically deboned chicken residue (MDCR) is an underutilized byproduct of poultry processing, which contains muscle and connective tissue proteins mixed with bones. Our previous study showed that enzymatic protein hydrolysis of MDCR facilitated separation of solubilized protein fraction from bones and produced diverse peptides, some with bioactive characteristics …”

“…Our previous study showed that enzymatic protein hydrolysis of MDCR facilitated separation of solubilized protein fraction from bones and produced diverse peptides, some with bioactive characteristics. 29…”