Multivariate analysis of biochemical responses using non-invasive methods to evaluate the health status of the endangered blackfin goodeid (Girardinichthys viviparus)

Dzul-Caamal, Ricardo; Olivares-Rubio, Hugo F.; Salazar‐Coria, L.; Rocha-Gómez, Maria Alejandra; Vega-López, Armando

doi:10.1016/j.ecolind.2015.09.017

Cited by 24 publications

(10 citation statements)

References 85 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The application of multivariate tools allowed discrimination of the most relevant predictors which outline unified fish biological responses to the effluent relative to location and seasons. It was also found valuable in other studies in monitoring the impact of complex mixtures of toxicants in aquatic ecosystems on fish species, and controlling conditions needed in validating and monitoring of fish health (Hashem Sweidan et al, 2015;Dzul-Caamal et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Native Prussian carp (Carassius gibelio) health status, biochemical and histological responses to treated wastewaters

Popović¹,

Strunjak‐Perović²,

Barišić³

et al. 2016

Environmental Pollution

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Native Prussian carp (Carassius gibelio) health status, biochemical and histological responses to treated wastewaters

Popović¹,

Strunjak‐Perović²,

Barišić³

et al. 2016

Environmental Pollution

View full text Add to dashboard Cite

“…The study of external fish mucus is becoming more popular as it provides nonlethal alternatives for both detecting fish infections and monitoring the environmental pollutants [29][30][31][32][33][34]. External fish mucus is most often sampled by gently scraping (e.g., with cell scraper or spatula) the external surfaces of a fish (body, fins, gills), avoiding ventral areas in order to avoid intestinal or sperm contaminations [45][46][47][48].…”

Section: Mucus Sampling and Analysismentioning

confidence: 99%

“…Fish mucus research has increased in the last ten years mainly due to the discovery of numerous bioactive molecules (antibacterial, antiviral, antifungal, and antiparasitic) and their potential application in human medicine and in aquaculture [26][27][28]. Furthermore, the study of external fish mucus provides nonlethal alternatives for the early detection of infections [29][30][31] and for monitoring the impact of environmental pollutants on fish health [32][33][34]. To date, most research on fish mucus has focused on immune-related molecules and AMPs, but few studies have analysed other mucus molecules and their ecological roles in the environment.…”

Section: Introductionmentioning

confidence: 99%

Biological and Ecological Roles of External Fish Mucus: A Review

Reverter

Tapissier‐Bontemps

Lecchini

et al. 2018

Fishes

184

137

View full text Add to dashboard Cite

Fish mucus layers are the main surface of exchange between fish and the environment, and they possess important biological and ecological functions. Fish mucus research is increasing rapidly, along with the development of high-throughput techniques, which allow the simultaneous study of numerous genes and molecules, enabling a deeper understanding of the fish mucus composition and its functions. Fish mucus plays a major role against fish infections, and research has mostly focused on the study of fish mucus bioactive molecules (e.g., antimicrobial peptides and immune-related molecules) and associated microbiota due to their potential in aquaculture and human medicine. However, external fish mucus surfaces also play important roles in social relationships between conspecifics (fish shoaling, spawning synchronisation, suitable habitat finding, or alarm signals) and in interspecific interactions such as prey-predator relationships, parasite–host interactions, and symbiosis. This article reviews the biological and ecological roles of external (gills and skin) fish mucus, discussing its importance in fish protection against pathogens and in intra and interspecific interactions. We also discuss the advances that “omics” sciences are bringing into the fish mucus research and their importance in studying the fish mucus composition and functions.

show abstract

“…Sea lice infestation increased the abundance of lectins in the skin mucus of Atlantic salmon (Easy and Ross, 2009 ), while transcriptional and proteomic approaches revealed differentially expressed proteins in the skin mucus of Atlantic cod upon natural infection with Vibrio anguillarum (Rajan et al, 2013 ). Likewise, metabolite profiling of fish skin mucus has been successfully applied as a novel approach for the monitoring and surveillance of wild fish health (Ekman et al, 2015 ; Dzul-Caamal et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Skin Mucus of Gilthead Sea Bream (Sparus aurata L.). Protein Mapping and Regulation in Chronically Stressed Fish

et al. 2017

View full text Add to dashboard Cite

The skin mucus of gilthead sea bream was mapped by one-dimensional gel electrophoresis followed by liquid chromatography coupled to high resolution mass spectrometry using a quadrupole time-of-flight mass analyzer. More than 2,000 proteins were identified with a protein score filter of 30. The identified proteins were represented in 418 canonical pathways of the Ingenuity Pathway software. After filtering by canonical pathway overlapping, the retained proteins were clustered in three groups. The mitochondrial cluster contained 59 proteins related to oxidative phosphorylation and mitochondrial dysfunction. The second cluster contained 79 proteins related to antigen presentation and protein ubiquitination pathways. The third cluster contained 257 proteins where proteins related to protein synthesis, cellular assembly, and epithelial integrity were over-represented. The latter group also included acute phase response signaling. In parallel, two-dimensional gel electrophoresis methodology identified six proteins spots of different protein abundance when comparing unstressed fish with chronically stressed fish in an experimental model that mimicked daily farming activities. The major changes were associated with a higher abundance of cytokeratin 8 in the skin mucus proteome of stressed fish, which was confirmed by immunoblotting. Thus, the increased abundance of markers of skin epithelial turnover results in a promising indicator of chronic stress in fish.

show abstract

Multivariate analysis of biochemical responses using non-invasive methods to evaluate the health status of the endangered blackfin goodeid (Girardinichthys viviparus)

Cited by 24 publications

References 85 publications

Native Prussian carp (Carassius gibelio) health status, biochemical and histological responses to treated wastewaters

Native Prussian carp (Carassius gibelio) health status, biochemical and histological responses to treated wastewaters

Biological and Ecological Roles of External Fish Mucus: A Review

Skin Mucus of Gilthead Sea Bream (Sparus aurata L.). Protein Mapping and Regulation in Chronically Stressed Fish

Contact Info

Product

Resources

About