Multivalent Traptavidin–DNA Conjugates for the Programmable Assembly of Nanostructures

Kim, Young-Youb; Bang, Yongbin; Lee, Ah-Hyoung; Song, Yoon‐Kyu

doi:10.1021/acsnano.8b06170

Cited by 23 publications

(18 citation statements)

References 61 publications

(80 reference statements)

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“…Traptavidin is a recently reported mutant of streptavidin with a lower koff rate for biotin binding versus streptavidin [13]. These mutations effectively increase the overall residence time of the complex with biotin, making Traptavidin a potentially more attractive alternative to streptavidin for biomaterials applications [20]. To test this, we constructed an expression cassette for an MPB-TEVS219V-TRPT fusion protein, expressing and immobilizing this protein fusion as previously described.…”

Section: Resultsmentioning

confidence: 99%

Covalent and non-covalent strategies for the immobilization of Tobacco Etch Virus protease (TEVp) on superparamagnetic nanoparticles

Norris

Patel

Dasari

et al. 2020

Journal of Biotechnology

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Section: Resultsmentioning

confidence: 99%

Covalent and non-covalent strategies for the immobilization of Tobacco Etch Virus protease (TEVp) on superparamagnetic nanoparticles

Norris

Patel

Dasari

et al. 2020

Journal of Biotechnology

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“…Compared with QD assemblies, NP-QD heterostructures were easily purified and concentrated by centrifugation and magnetic separation. [16,34,51] Plasmonic metal NPs and semiconductor heterostructures were obtained by optimizing the DNA sequences and concentrations on each NP. For example, by controlling the DNA density to ≈0.28 DNA per nm 2 on Au NPs, about six or seven QDs were assembled on every Au NP (Figure 3a).…”

Section: Plasmonic Metal Nps and Semiconductor Heterostructuresmentioning

confidence: 99%

“…DNA molecules contain abundant functional groups and unique biometric recognition capabilities, which allow for the precise modification and programmable construction of NP assemblies. [ 16–18 ] DNA can be self‐assembled into desired 3D shapes, [ 19 ] and the configuration of plasmonic heterogeneous nanoassemblies can be controlled by adjusting the sequence, length, and configuration of DNA. [ 20 ] Oriented self‐assembly holds great promise for controlling the morphologies of nanostructures beyond what is possible using conventional systems.…”

Section: Introductionmentioning

confidence: 99%

DNA‐Based Plasmonic Heterogeneous Nanostructures: Building, Optical Responses, and Bioapplications

Zhao

2020

Advanced Materials

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The integration of multiple functional nanoparticles into a specific architecture allows the precise manipulation of light for coherent electron oscillations. Plasmonic metals-based heterogeneous nanostructures are fabricated by using DNA as templates. This comprehensive review provides an overview of the controllable synthesis and self-assembly of heterogeneous nanostructures, and analyzes the effects of structural parameters on the regulation of optical responses. The potential applications and challenges of heterogeneous nanostructures in the fields of biosensors and bioanalysis, in vivo monitoring, and phototheranostics are discussed. 1907880 (2 of 22) www.advmat.de www.advancedsciencenews.com 2.1. Plasmonic Metal NP Heterostructures Heterostructures consisting of distinct metal NPs can give rise to targeted LSPR properties, and the plasmonic coupling of nano-objects between plasmonic metal NPs enhances EM fields. The intense EM enhancement gives rise to intense optical responses, which is dependent on sizes, shapes, and even the orientation of constituent NPs. [2] Plasmonic metallic NPs, especially Au and Ag NPs, possess tunable LSPR over a broad spectral range from the visible to the NIR regions and Yuan Zhao is an associate professor at Jiangnan University. She received her Ph.D. degree at Jiangnan University in 2013, supervised by Prof. Chuanlai Xu. She was a postdoctoral fellow at the Chinese University of Hong Kong in 2019, directed by Prof. Jianfang Wang. Her current research focuses on functional nanomaterials with optical and electrochemical properties for applications.

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“…Techniques using nanochannels, 15-17 magnetic nanobeads, 18,19 evaporation 20 and Langmuir-54 Blodgett films 21 have been explored to concentrate and deposit suspended particles to surfaces, but depositing them precisely to designated locations, which is important for applications like multiplexing sensors, are still challenging. Previous studies show that fluid flow around an photothermally generated surface bubble can be a promising deposition methods with precision.…”

Section: Introductionmentioning

confidence: 99%

Biocompatible Direct Deposition of Functionalized Nanoparticles using Shrinking Surface Plasmonic Bubble

Moon¹,

Zhang²,

Huang³

et al. 2020

Preprint

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<p>Functionalized nanoparticles (NPs) are the foundation of diverse applications, such as photonics, composites, energy conversion, and especially biosensors. In many biosensing applications, concentrating the higher density of NPs in the smaller spot without deteriorating biofunctions is usually an inevitable step to improve the detection limit, which remains to be a challenge. In this work, we demonstrate biocompatible deposition of functionalized NPs to an optically transparent surface using shrinking surface plasmonic bubbles. Leveraging the shrinking bubble can enable to mitigate any potential biomolecules degradation by strong photothermal effect, which has been a big obstacle of bridging plasmonic bubbles with biomolecules. The deposited NPs are closely packed in a micro-sized spot (as small as 3 μm), and the functional molecules are able to survive the process as verified by their strong fluorescence signals. We elucidate that the contracting contact line of the shrinking bubble forces the NPs captured by the contact line to a highly concentrated island. Such a shrinking surface bubble deposition (SSBD) is low temperature in nature as no heat is added during the process. Using a hairpin DNA-functionalized gold NP suspension as a model system, SSBD is shown to enable much stronger fluorescence signal compared to the optical pressure deposition and the conventional steady thermal bubble contact line deposition. The demonstrated SSBD technique capable of directly depositing functionalized NPs may benefit a wide range of applications, such as the manufacturing of multiplex biosensors.</p>

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Multivalent Traptavidin–DNA Conjugates for the Programmable Assembly of Nanostructures

Cited by 23 publications

References 61 publications

Covalent and non-covalent strategies for the immobilization of Tobacco Etch Virus protease (TEVp) on superparamagnetic nanoparticles

Covalent and non-covalent strategies for the immobilization of Tobacco Etch Virus protease (TEVp) on superparamagnetic nanoparticles

DNA‐Based Plasmonic Heterogeneous Nanostructures: Building, Optical Responses, and Bioapplications

Biocompatible Direct Deposition of Functionalized Nanoparticles using Shrinking Surface Plasmonic Bubble

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