Multitarget Reaction Programmable Automatic Diagnosis and Treatment Logic Device

Abstract

Accurate diagnosis and precise and effective treatment are currently the two magic weapons for dealing with cancer. However, a single marker is often associated with multiple cellular events, which is not conducive to accurate diagnosis, and overly mild treatment methods often make the treatment effect unsatisfactory. In this paper, we construct a Au/Pd octopus nanoparticle–DNA nanomachine (Au/Pd ONP–DNA nanomachine) as a fully automatic diagnosis and treatment logic system. In this system, multiple DNA components are targeting detection units, Au/Pd ONPs act as carriers, and Au/Pd ONPs with an 808 nm laser is the treatment unit. In order to achieve the purpose of precise treatment, we will detect two secondary markers under the premise of detecting one major tumor marker. When all of the designated targets are detected (the logic system input is (1, 1, 1), and the output is (1, 1)), the 808 nm laser can be programmed to automatically radiate tumors and perform photothermal therapy and photodynamic therapy. In vivo and in vitro experiments show that this logic system not only can accurately identify tumor cells but also has considerable therapeutic effects.

A covalently conjugated branched DNA aptamer cluster-based nanoplatform for efficiently targeted drug delivery

“…Two kinds of cell lines (MUC1 positive MCF-7 and MUC1 negative L929) were chosen for the evaluation of targeted cellular internalization. 69 As shown in Fig. 4a, a much enhanced cellular uptake was observed in the TET + 5Apt group compared to the free TET and TET + 1Apt groups by confocal imaging analysis of treated MCF-7 cells.…”

PAI/MRI Visualization of Tumor Derived Cellular Microvesicles with Endogenous Biopolymer Nanoparticles Modification

“…For PA imaging in vitro, a phantom filled with TDMVs+MNP-Gd in different concentrations of MNP (25,50,100,200, and 400 μg/mL) was measured using a real-time multispectral optoacoustic tomographic (MSOT) imaging system (inVision 128, iThera Medical GmbH, Neuherberg, Germany). Finally, the PA signals of each sample were measured in regions of interest (ROIs), and the correlation between the PA signal and concentration curve was calculated.…”

“… 22–24 With the rapid development of nanotechnology and molecular imaging, engineering nanoparticles are expected to be potential candidates for visualizing the localization and functional behavior of TDMVs. 25 …”

“…[22][23][24] With the rapid development of nanotechnology and molecular imaging, engineering nanoparticles are expected to be potential candidates for visualizing the localization and functional behavior of TDMVs. 25 Among all non-invasive molecular and functional imaging techniques, magnetic resonance imaging (MRI) has been widely used in the biomedical field as a non-invasive technique with the advantages of high spatiotemporal resolution and good soft tissue contrast. 26,27 Contrast agents could enhance the relaxation of protons in water, which is of great significance for continuously improving the MRI contrast of different tissues and organs and achieving accurate detection of diseases.…”

Fluorescent Detection of Two Pesticides Based on CRISPR-Cas12a and Its Application for the Construction of Four Molecular Logic Gates

“…Different input combinations will result in different outputs according to the truth table permutation . Recently, some excellent logic gates were fabricated on the basis of molecular computations, such as AND, OR, XOR, NAND, half adder, half subtractor, etc. − Most of the previously reported logic gates employed nucleic acids, , proteins, , and heavy metal ions , as inputs. There is still no report about the logic gate for pesticide applications.…”