Multitarget Effects of Danqi Pill on Global Gene Expression Changes in Myocardial Ischemia

Abstract

Danqi pill (DQP) is a widely prescribed traditional Chinese medicine (TCM) in the treatment of cardiovascular diseases. The objective of this study is to systematically characterize altered gene expression pattern induced by myocardial ischemia (MI) in a rat model and to investigate the effects of DQP on global gene expression. Global mRNA expression was measured. Differentially expressed genes among the sham group, model group, and DQP group were analyzed. The gene ontology enrichment analysis and pathway analysis of differentially expressed genes were carried out. We quantified 10,813 genes. Compared with the sham group, expressions of 339 genes were upregulated and 177 genes were downregulated in the model group. The upregulated genes were enriched in extracellular matrix organization, response to wounding, and defense response pathways. Downregulated genes were enriched in fatty acid metabolism, pyruvate metabolism, PPAR signaling pathways, and so forth. This indicated that energy metabolic disorders occurred in rats with MI. In the DQP group, expressions of genes in the altered pathways were regulated back towards normal levels. DQP reversed expression of 313 of the 516 differentially expressed genes in the model group. This study provides insight into the multitarget mechanism of TCM in the treatment of complex diseases.

Danqi soft capsule prevents infarct border zone remodelling and reduces susceptibility to ventricular arrhythmias in post‐myocardial infarction rats

“…The Chinese herb combination S miltiorrhiza and P notoginseng is one of the most widely prescribed formulas, and has remarkable protective effects on heart failure and coronary heart disease . The present study demonstrated the considerable long‐term cardiac protective effects of this herb combination (DQ) in IBZ remodelling, decreasing susceptibility to VT in post‐MI rats.…”

“…24,25 The present study demonstrated the considerable long-term cardiac protective effects of this herb combination (DQ) in IBZ remodelling, decreasing susceptibility to VT in post-MI rats. The current results showed that DQ inhibited fibrosis and myocyte hypertrophy, and increased Cx43 expression in the IBZ.…”

“…E, DQ reduced the protein levels of collagen III as assessed by Western blot (n = 5 rats/group). *P < 0.05, **P < 0.01 vs Sham group, # P < 0.05, ## P < 0.01 vs MI group4 | D ISCUSS I ONThe Chinese herb combination S miltiorrhiza and P notoginseng is one of the most widely prescribed formulas, and has remarkable protective effects on heart failure 10 and coronary heart disease 24,25. The present study demonstrated the considerable long-term cardiac protective effects of this herb combination (DQ) in IBZ remodelling, decreasing susceptibility to VT in post-MI rats.…”

The Effect of Chinese Medicine on Lipid and Glucose Metabolism in Acute Myocardial Infarction Through PPARγ Pathway

“…Most recently, the imbalance of energy metabolism has become an attractive target for the treatment of HF after AMI ( Noordali et al, 2017 ). Accumulating studies integrating transcriptome, proteomics, and metabolomics have revealed that energy metabolism disorders dominated in HF ( Wang et al, 2018 ). Disturbance of glucose and lipid metabolism is the fundamental energy metabolism disorder after AMI, which can directly lead to the HF ( Heggermont et al, 2016 ).…”

Systems biology approaches in the study of Chinese herbal formulae

“…This study demonstrates the feasibility of microarray technology in combination with other techniques for the evaluation of therapeutic efficiencies and mechanism studies of CHF. Wang et al [20] reported the mechanism of the Danqi pill (DQP) in myocardial ischemia treatment by measuring gene microarrays in a rat model. After evaluating the effects of signaling pathways and metabolism, the authors found that DQP can reverse energy metabolic disorders to recover cardiac function.…”