Multistressor interactions in the zebrafish (Danio rerio): Concurrent phenanthrene exposure and Mycobacterium marinum infection

Prosser, Christopher M.; Unger, Michael A.; Vogelbein, Wolfgang K.

doi:10.1016/j.aquatox.2011.01.011

Cited by 23 publications

(16 citation statements)

References 39 publications

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“…It is plausible that a Th1 reduction and tilt towards a Th2 response in BB 2011, if biologically significant despite not being statistically significant, would increase the susceptibility of those dolphins to Brucella infection or other intracellular pathogens. Increased susceptibility to bacterial challenges, including intra-cellular Mycobacterium marinum, upon PAH exposure was specifically confirmed in several studies in fish (Bravo et al 2011, Prosser et al 2011.…”

Section: Oil Exposure Immune Functions and Perinatal Mortalitiesmentioning

confidence: 81%

Changes in immune functions in bottlenose dolphins in the northern Gulf of Mexico associated with the Deepwater Horizon oil spill

Guise¹,

Levin²,

Gebhard³

et al. 2017

Endang. Species. Res.

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Following the Deepwater Horizon (DWH) oil spill, the Natural Resource Damage Assessment conducted comprehensive health assessments on common bottlenose dolphins Tursiops truncatus in Barataria Bay (BB), Louisiana, in 2011Louisiana, in , 2013Louisiana, in and 2014, as well as in Mississippi Sound (MS) in 2013, to assess potential health effects from exposure to oil compared to Sarasota Bay (SB), Florida dolphins not exposed to DWH oil. Immune functions demonstrated a consistent increase in T (BB 2011) and B (BB 2011 and lymphocyte proliferation compared to SB. Cytokine concentrations varied considerably in wild dolphin populations, and no significant differences were found; however, interesting trends were observed. The Th1 cytokines IL-2, IL-12, and IFNγ, and the Th2 cytokines IL-5, IL-10, and IL-13, were 2-to 50-fold lower, and IL-4 was 3-fold higher, in BB 2011 compared to SB. Overall, the changes observed were compatible with those documented in other species following exposure to oil or PAHs and were most pronounced in BB 2011, at the place and time most affected by oil. The changes in T cell functions, and the trend towards a cytokine balance tilted towards a Th2, rather than a Th1 response, are compatible with intra-cellular bacterial infections such as Brucella, which has been identified as one of the potential contributory factors to perinatal dolphin mortalities, and changes in B cell functions are compatible with an increase in extra-cellular bacterial infections and primary bacterial pneumonia.

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Section: Oil Exposure Immune Functions and Perinatal Mortalitiesmentioning

confidence: 81%

Changes in immune functions in bottlenose dolphins in the northern Gulf of Mexico associated with the Deepwater Horizon oil spill

Guise¹,

Levin²,

Gebhard³

et al. 2017

Endang. Species. Res.

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“…Stressors, including poor water quality, handling and high stocking densities, are associated with increased disease prevalence (Ramsay et al . ; Prosser, Unger & Vogelbein ), and a link to genetic susceptibility has also been suggested (Broussard & Ennis ). Although all fish species are considered susceptible to mycobacteriosis, members of the families Anabantidae, Characidae, Cyprinidae, Cichlidae and Syngnathidae appear to have increased susceptibility (Noga, Wright & Pasarell ; Decostere et al .…”

Section: Introductionmentioning

confidence: 98%

“…Disease caused by NTM occurs infrequently in immune compromised humans, but many fish species are readily susceptible and infections are often fatal (Gauthier & Rhodes 2009). Stressors, including poor water quality, handling and high stocking densities, are associated with increased disease prevalence (Ramsay et al 2009;Prosser, Unger & Vogelbein 2011), and a link to genetic susceptibility has also been suggested (Broussard & Ennis 2007). Although all fish species are considered susceptible to mycobacteriosis, members of the families Anabantidae, Characidae, Cyprinidae, Cichlidae and Syngnathidae appear to have increased susceptibility (Noga, Wright & Pasarell 1990;Decostere et al 2004;Berzins & Greenwell 2005;Novotny et al 2010;Reavill & Schmidt 2012).…”

Section: Introductionmentioning

confidence: 99%

Pathologic features of mycobacteriosis in naturally infected Syngnathidae and novel transcriptome assembly in association with disease

Fogelson

Fast

Leary

et al. 2017

Journal of Fish Diseases

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Syngnathidae (seahorses, seadragons and pipefish) suffer significant losses from non-tuberculous mycobacteria. However, they produce markedly different lesions in response to the disease compared to other teleost species, notably infrequent granuloma formation. This study evaluated 270 syngnathid fish, from which 92 were diagnosed with mycobacteriosis by histopathology, culture or both. Microscopic lesions variably consisted of random foci of coagulative necrosis in multiple organs, containing high numbers of free bacteria and large aggregates or sheets of macrophages with cytoplasm laden with acid-fast bacilli. Mycobacterial associated granulomas were identified in only six seahorses. Five fish had positive cultures with no observed microscopic changes. RNA-seq of the head kidney was performed to investigate the transcriptome of two infected and six non-infected lined seahorses Hippocampus erectus. Assembled and annotated putative transcripts serve to enrich the database for this species, as well as provide baseline data for understanding the pathogenesis of mycobacteriosis in seahorses. Putative components of the innate immune system (IL-1β, IL-6, TNF, NOS, Toll-like receptor 1, MHC Class I, NF-κβ, transforming growth factor beta, MyD88) were identified in the RNA-seq data set. However, a homolog for a key component in the TH1 adaptive immune response, interferon-gamma, was not identified and may underlie the unique pathologic presentation.

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“…23 Despite limited aqueous solubility (1.28 μg ml −1 ), 23 phenanthrene as a model PAH exhibits profound ZFE toxicities in their aqueous culture model at 10 μg View Article Online ml −1 . Phenanthrene is a known toxin with reported toxicities in Danio rerio reporting an LD 50 of 0.293 μg ml −1 when exposed to adult fish for 14 days.…”

Section: Discussionmentioning

confidence: 99%

Carbon nanomaterials rescue phenanthrene toxicity in zebrafish embryo cultures

Falconer

Jones

et al. 2015

Environ. Sci.: Nano

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Multi-Walled Carbon Nanotubes (MWCNTs) and Carbon Blacks (CB) are known to carry wide varieties of adsorbed, potentially toxic chemicals resulting from their manufacturing, typically including metals and polyaromatic hydrocarbons (PAHs). Instead of desorption of these adsorbates into aqueous milieu, substantial high-affinity adsorption of the aqueous dispersed PAH, phenanthrene to MWCNTs and CB from aqueous solutions and dispersions is reported. Phenanthrene adsorption to aqueous dispersed carbon nanomaterials phases was measured using isotherms and then exploited to remove toxic levels of phenanthrene from aqueous media incubating zebrafish embryos (ZFEs) as a whole organism toxicity screening system. Remarkable reduction in phenanthrene-induced ZFE toxicity was observed using two experiments: one comparing PAH concentration-dependent rescue of ZFE viability from MWCNTs compared to carbon black (CB), and a second examining kinetics of the ZFE rescue by MWCNTs vs. CB incubations after initial ZFE exposure to phenanthrene for 2 hours. Phenanthrene LD 50 concentration in the absence of any carbon-based sorbent increases dramatically to 10 μg ml −1 in the presence of either 81.3 μg ml −1 MWCNTs or 81.5 μg ml −1 CB materials. When CB and MWCNTs were added to ZFEs previously exposed to 10 μg ml −1 phenanthrene for 2 hours, significant rescue of ZFE viability was observed in CB-treated embryos while no ZFE rescue was observed in MWCNT-treated ZFEs. This result is consistent with an expected carbon nanomaterial surface area-dependent ZFE rescue effect: CB exhibits a higher Brunauer-Emmett-Teller (BET) surface area than the MWCNTs used, with higher adsorption capacity likely for phenanthrene, yielding ZFE rescue from toxicity.Environ. Sci.: Nano This journal is Nanotoxicity is an increasing concern for public health given the increasing number of new engineered nanomaterials introduced into consumer products and the environment with potential for wide dispersal. Carbon-based nanomaterials represent a major class of engineered nanomaterials with numerous possible interactions with the environment and living systems. This whole organism toxicity study evaluates the zebrafish embryo toxicity to the common environmental polyaromatic hydrocarbon toxin, phenanthrene, and reports toxicity rescue mediated by multi-walled carbon nanotubes and carbon blacks added to the culture milieu.

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Multistressor interactions in the zebrafish (Danio rerio): Concurrent phenanthrene exposure and Mycobacterium marinum infection

Cited by 23 publications

References 39 publications

Changes in immune functions in bottlenose dolphins in the northern Gulf of Mexico associated with the Deepwater Horizon oil spill

Changes in immune functions in bottlenose dolphins in the northern Gulf of Mexico associated with the Deepwater Horizon oil spill

Pathologic features of mycobacteriosis in naturally infected Syngnathidae and novel transcriptome assembly in association with disease

Carbon nanomaterials rescue phenanthrene toxicity in zebrafish embryo cultures

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