Multistrain Probiotics with Fructooligosaccharides Improve Middle Cerebral Artery Occlusion–Driven Neurological Deficits by Revamping Microbiota-Gut-Brain Axis

Rahman, Ziaur; Bhale, Nagesh A.; Dikundwar, Amol G.; Dandekar, Manoj P.

doi:10.1007/s12602-023-10109-y

Cited by 8 publications

(4 citation statements)

References 125 publications

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“…The genomic bacterial DNA was extracted from fecal samples using a QIAamp PowerFecal Pro DNA Kit (Qiagen catalog no: 51804). The procedure for the extraction of DNA from rodent fecal samples has been standardized and published in our earlier studies ( Rahman et al, 2023 ). Briefly, 200–250 mg of fecal sample was added to the PowerBead Pro Tubes containing 800 μL of CD1 solution and then homogenized using a FastPrep-24™ bead grinder (MP Biomedicals, United States) followed by centrifugation at 15,000 × g for 1 min.…”

Section: Methodsmentioning

confidence: 99%

Fecal microbiota transplantation unveils sex-specific differences in a controlled cortical impact injury mouse model

Pasam,

Dandekar

2024

Front. Microbiol.

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IntroductionContusion type of traumatic brain injury (TBI) is a major cause of locomotor disability and mortality worldwide. While post-TBI deleterious consequences are influenced by gender and gut dysbiosis, the sex-specific importance of commensal gut microbiota is underexplored after TBI. In this study, we investigated the impact of controlled cortical impact (CCI) injury on gut microbiota signature in a sex-specific manner in mice.MethodsWe depleted the gut microflora of male and female C57BL/6 mice using antibiotic treatment. Thereafter, male mice were colonized by the gut microbiota of female mice and vice versa, employing the fecal microbiota transplantation (FMT) method. CCI surgery was executed using a stereotaxic impactor (Impact One™). For the 16S rRNA gene amplicon study, fecal boli of mice were collected at 3 days post-CCI (dpi).Results and discussionCCI-operated male and female mice exhibited a significant alteration in the genera of Akkermansia, Alistipes, Bacteroides, Clostridium, Lactobacillus, Prevotella, and Ruminococcus. At the species level, less abundance of Lactobacillus helveticus and Lactobacillus hamsteri was observed in female mice, implicating the importance of sex-specific bacteriotherapy in CCI-induced neurological deficits. FMT from female donor mice to male mice displayed an increase in genera of Alistipes, Lactobacillus, and Ruminococcus and species of Bacteroides acidifaciens and Ruminococcus gnavus. Female FMT-recipient mice from male donors showed an upsurge in the genus Lactobacillus and species of Lactobacillus helveticus, Lactobacillus hamsteri, and Prevotella copri. These results suggest that the post-CCI neurological complications may be influenced by the differential gut microbiota perturbation in male and female mice.

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Section: Methodsmentioning

confidence: 99%

Fecal microbiota transplantation unveils sex-specific differences in a controlled cortical impact injury mouse model

Pasam,

Dandekar

2024

Front. Microbiol.

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“…We identified 140 articles, of which 32 articles were deemed irrelevant to our research topic, nine were reviews, two were letters to the editor, one was an editorial, one was a perspective piece, one was a comment, one was a duplicate publication, and one was an un‐peer‐reviewed preprint; these were excluded by two authors who independently assessed the articles. Ultimately, 92 studies, 5–96 including 38 human studies and 54 animal studies, were selected as being highly relevant to our research topic. The flowchart of the article selection process is depicted in Figure 1.…”

Section: Methodsmentioning

confidence: 99%

“…Rahman et al 85 Lactobacillus reuteri UBLRu-87, Lactobacillus plantarum UBLP-40, Lactobacillus rhamnosus UBLR-58, Lactobacillus salivarius UBLS-22, Bifidobacterium breve UBBr-01…”

Section: Study Probiotics Potential Mechanismmentioning

confidence: 99%

Role of gut microbiota in ischemic stroke: A narrative review of human and animal studies

Chen,

Du,

Cui

et al. 2024

Neuroprotection

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The high incidence, mortality, and disability associated with ischemic stroke pose a significant threat to human health. The intestinal microbiota significantly influences the onset, progression, and prognosis of ischemic stroke. Gut flora plays a pivotal role in brain–gut interactions. The reflection of changes in the gut and brain caused by gut microbes facilitates the investigation of early warning biomarkers and potential therapeutic targets for ischemic stroke. In this narrative review of the relationship between gut microbiota and ischemic stroke, we primarily discuss three topics, grounded in real‐world human and animal studies. First, we examined the relationship between ischemic stroke and intestinal microbiota and its metabolites, delineate the overall characteristics of intestinal microbiota dysregulation in ischemic stroke, and assess the potential clinical value, prevailing research controversies, and unique phenomena of intestinal microbiota metabolites such as trimethylamine N‐oxide and short‐chain fatty acids in ischemic stroke. Second, we explored the potential communication pathways between intestinal flora and ischemic stroke based on the brain–gut axis, encompassing metabolic pathways, immune pathways, and neural pathways. Finally, we encapsulated the factors influencing the severity of ischemic stroke via intestinal flora, the pharmacological and nonpharmacological interventions that modulate intestinal flora in treating ischemic stroke, and the current research landscape of intestinal flora in the context of ischemic stroke sequelae.

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“…The HPA axis is activated by CRH and ACTH when pathogenic infections arise (Fan et al, 2023). Studies show that those who experience stress or anxiety have dysregulated gut flora, which alters the HPA axis (Rahman et al, 2023). Changing the microbiota in the gut may help treat and alleviate mental diseases because of the significant role the gut-brain axis plays in these ailments (Wu, Wang, Tang, et al, 2022).…”

Section: Neuronal Pathways For Gut-brain Axis Interactionsmentioning

confidence: 99%

Probiotics: Bridging the interplay of a healthy gut and psychoneurological well‐being

Meher,

Acharya,

Sahu

2024

Food Bioengineering

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The establishment of a healthy microbiota, particularly during infancy, profoundly influences psychological health and neurological function through the gut‐brain axis. This review delves into the intricate connections between probiotics, gut microbiome development, and their impact on neurological disorders. Biotics, live microorganisms with proven health benefits, have emerged as a promising intervention, particularly during critical developmental stages. Administering specific probiotic strains (Lactobacillus species and Bifidobacterium) in infancy has shown promise in preventing and alleviating gut disorders, with implications for psychological well‐being. The bidirectional communication along the gut‐brain axis underscores the potential of probiotics in influencing neurological outcomes, ranging from anxiety to neurodevelopmental disorders. Additionally, this review explores emerging food engineering techniques (microencapsulation, genome editing, fermentation, protein engineering, immobilization, etc.) employed in preparing probiotic‐based foods, ensuring the viability and targeted release of probiotics in the gastrointestinal tract.

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Multistrain Probiotics with Fructooligosaccharides Improve Middle Cerebral Artery Occlusion–Driven Neurological Deficits by Revamping Microbiota-Gut-Brain Axis

Cited by 8 publications

References 125 publications

Fecal microbiota transplantation unveils sex-specific differences in a controlled cortical impact injury mouse model

Fecal microbiota transplantation unveils sex-specific differences in a controlled cortical impact injury mouse model

Role of gut microbiota in ischemic stroke: A narrative review of human and animal studies

Probiotics: Bridging the interplay of a healthy gut and psychoneurological well‐being

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