Multistep Synthesis of (S)‐3‐Hydroxyisobutyric Acid from Glucose usingPseudomonas taiwanensisVLB120 B83 T7 Catalytic Biofilms

Abstract

Thec hiral building block (S)-3-hydroxyisobutyric acid [(S)-3-HIBA] was produced either by conversion of isobutyric acid or directly from glucose utilizing cells of Pseudomonas taiwanensis VLB120 B83 T7 as catalysts.T his strain carriesapoint mutation in the gene encoding 3-hydroxyisobutyrate dehydrogenase (mmsB), leading to its inactivation.T he maximal specific activity in resting-cell biotransformations using isobutyric acid as substrate was 4.9 AE 0.4 Ug cdw À1 .O verexpression of the 2-ketoisovalerate pathwayg enes alsS,i lvC, and ilvD,a nd the introduction of kivd encoding 2-ketoacid decarboxylase resulted in the efficient fermentatives ynthesiso f( S )-3-HIBAd irectly from glucose.U pt o2 2mM( 2.3 g L À1 ) (S)-3-HIBAwere produceda t3 .7 AE 0.3 Ug cdw À1 in repeated batch experiments without observable product degradation. Utilizing ab iofilm reactor it was possible to continuously produce up to.O verall, the conversion of isobutyric acid to (S)-3-HIBAw as found to be the rate-limiting step,l eading to the accumulation of am ixture of (S)-3-hydroxyisobutyric acid andi sobutyric acid. This study demonstratesf or the first time the production of (S)-3-HIBAf rom renewable carbon in shake flasks and biofilm reactors and sets the stage for furthero ptimizations towards the efficient production of 3-HIBAa nd its derivatives in continuous fermentations.

Isobutyrate biosynthesis via methanol chain elongation: converting organic wastes to platform chemicals

“…We have identified isobutyrate as one of the interesting chemicals that are to be produced through chain elongation. Isobutyrate, though not a MCFA, is a useful platform chemical that can be used as a precursor for: (1) methyl methacrylic acid (MMA), which is used for production of transparent thermoplastics with an annual market size of up to 2.2 million tonnes; (2) sucrose acetate isobutyrate, which is used as an emulsifier in many commodities with an annual market size of up to 100 000 tonnes; or (3) 3‐hydroxyisobutyric acid that can be used for synthesis of vitamins, antibiotics and many other bioactive compounds . A recent study also showed that isobutyrate could be biologically reduced in a syngas fermentation process to yield isobutanol, a commercially applied fuel substitute that has a superior performance over ethanol .…”

Continuous multistep synthesis of perillic acid from limonene by catalytic biofilms under segmented flow

“…In conclusion, P. putida GS1 is superior in terms of resilience to both limonene and perillic acid as compared to P. taiwanensis VLB120. Nevertheless, P. taiwanensis VLB120 is a well‐characterized biofilm producer and process parameters have been described for the biofilm‐based production of toxic compounds such as ( S )‐styrene oxide (Gross et al, ; Halan et al, ), octanol (Gross et al, ), and ( S )‐3‐hydroxyisobutyric acid (Lang et al, ). Hence, P. taiwanensis VLB120 was still included as a potential production strain for perillic acid.…”

Screening of Altered Metabolites and Metabolic Pathways in Celiac Disease Using NMR Spectroscopy

“…Gut microbiota productions can be absorbed by the colonic epithelium; they enter the bloodstream and play an important role in regulating the metabolism of glucose, fatty acids, and cholesterol. Changes in serum metabolite levels in CeD have been identified and suggest significant changes in the gut microbiota of CeD subjects [60][61][62][63][64][65].…”