Multistage Carcinogenesis: Cell and Animal Models

Kulesz‐Martin, Molly; Gallegos, Jayme R.; Liu, Y.

doi:10.1016/b978-0-08-046884-6.01402-0

Cited by 1 publication

(1 citation statement)

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“…[41] Specifically, normal epidermal homeostasis, in the context of KC, requires the expression of calcium-binding proteins, like cadherins, to facilitate the terminal differentiation of KC. [42] In psoriasis, where the barrier is defective, there is a global increase in calcium content. [41] We have found that high concentration of Ca 2+ at 1 mmol/L exhibited the loss of the filopodia formation in MC ( Figure 3A), and subsequently, there was inhibition in melanin transfer (Figure 3B, C).…”

Section: Ca 2+ Induced Melanocyte Filopodia Formation and Melanin Tmentioning

confidence: 99%

E‐cadherin mediates ultraviolet radiation‐ and calcium‐induced melanin transfer in human skin cells

Singh

Baker

Sikkink

et al. 2017

Experimental Dermatology

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Abstract:Skin pigmentation is directed by epidermal-melanin units, characterized by long-lived and dendritic epidermal melanocytes (MC) that interact with viable keratinocytes (KC) to contribute melanin to the epidermis. Previously we reported that MC:KC contact is required for melanosome transfer, that this can be enhanced by filopodial and by UVR/UVA irradiation, which can up-regulate melanosome transfer via Myosin X-mediated control of MC filopodia. Both MC and KC express Ca 2+ -dependent E-cadherins. These homophilic adhesion contacts induce transient increases in intra-KC Ca 2+, while ultraviolet radiation (UVR) raises intra-MC Ca 2+ via calcium selective ORAI1 ion channels; both are associated with regulating melanogenesis.However, how Ca 2+ triggers melanin transfer remains unclear, and here we evaluated the role of E-Cadherin in UVR-mediated melanin transfer in human skin cells. MC and KC in human epidermis variably express filopodia-associated E-Cadherin, Cdc42, VASP and β-catenin, all of which were upregulated by UVR/UVA in human MC in vitro. Knockdown of Ecadherin revealed that this cadherin is essential for UVR-induced MC filopodia formation and melanin transfer. Moreover, Ca 2+ induced a dose-dependent increase in filopodia formation and melanin transfer, as well as increased β-catenin, Cdc42, Myosin X, and E-Cadherin Accepted ArticleThis article is protected by copyright. All rights reserved.

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Section: Ca 2+ Induced Melanocyte Filopodia Formation and Melanin Tmentioning

confidence: 99%