Multispectroscopic analysis and molecular modeling to investigate the binding of beta lactoglobulin with curcumin derivatives

Abstract

Bovine beta lactoglobulin (β-lg), the major whey protein, has a great affinity for a wide range of organic compounds like fatty acids, retinol etc.

Binding behaviors and structural characteristics of ternary complexes of β-lactoglobulin, curcumin, and fatty acids

“…Curcumin located near Trp19, Tyr20, Thr125, Pro126, Glu158 and Glu44 through both hydrophobic interactions and hydrogen bonds, which was similar to the binding site in b-lg-resveratrol and b-lg-isoxazole interactions. 39,40 Moreover, the binding mode for curcumin out of the b-lg calyx was still stable when solvation and exibility was present in the biological system (Fig. S6 †).…”

Partitioning Behavior and Interfacial Activity of Phenolic Acid Derivatives and their Impact on β-Lactoglobulin at the Oil-Water Interface

“…For β-lg, it is possible to bind PADs inside its hydrophobic calyx, which is unfolding/exposing at the interface. Possible amino acids in the calyx for π-interactions are Phe 105 , Asn 90 , Ile 84 , Ile 56 , Val 43 , Val 92, and Met 107 residues [29]. Interactions in the calyx may have an important impact on the adsorption and unfolding behavior of β-lg at the interface as demonstrated in this study.…”

“…In the present study, β-lactoglobulin was used as a model protein since its interfacial behavior is well characterized. Previous studies on β-lactoglobulin and phenolic compound (curcumin) (reference) showed that hydrogen bonds, π-stacking, and other π-interactions like π-alkyl, π-anion, and π-sigmaas a kind of hydrophobic interactionsare the main forces for this proteinphenol bonding [29].…”

Facile Synthesis of a New Class of Pyrazolone Attached Chromene Derivatives Showing Good Binding with β‐Lactoglobulin

“…Also, the fluorescence intensity of the protein was found to depend on the concentration of 4 which was analyzed by using the Stern–Volmer plots. Thus, the plots of F 0 /F versus concentration of 4 gave a straight line with an intercept as shown in the insets of the plots given in the Supporting Information (SI) (where F 0 is the fluorescence intensity at λ max without addition of 4 and F is the fluorescence intensity at λ max with different concentrations of 4 ) ,. From this plot, for the compounds 4 c (shown in the inset of Figure ) the binding constants was calculated to be 16.3×10 5 M −1 .…”