Multispectral confocal microendoscope for in vivo and in situ imaging

Makhlouf, Houssine; Gmitro, Arthur F.; Tanbakuchi, Anthony; Udovich, Joshua A.; Rouse, Andrew R.

doi:10.1117/1.2950313

Cited by 53 publications

(40 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We expect this technique to be applicable to cancer excisions beyond the skin such as in excisions of oral mucosal lesions, thyroid nodules, parathyroid glands, and bone during head-and-neck surgery, needle core-biopsies and lumpectomies of breast, and intra-operative biopsies of liver, kidney and bladder that have been imaged with confocal microscopy. [17][18][19] …”

Section: Discussionmentioning

confidence: 99%

Tri-modal confocal mosaics detect residual invasive squamous cell carcinoma in Mohs surgical excisions

et al. 2012

View full text Add to dashboard Cite

Abstract. For rapid, intra-operative pathological margin assessment to guide staged cancer excisions, multimodal confocal mosaic scan image wide surgical margins (approximately 1 cm) with sub-cellular resolution and mimic the appearance of conventional hematoxylin and eosin histopathology (H&E). The goal of this work is to combine three confocal imaging modes: acridine orange fluorescence (AO) for labeling nuclei, eosin fluorescence (Eo) for labeling cytoplasm, and endogenous reflectance (R) for marking collagen and keratin. Absorption contrast is achieved by alternating the excitation wavelength: 488 nm (AO fluorescence) and 532 nm (Eo fluorescence). Superposition and false-coloring of these modes mimics H&E, enabling detection of cutaneous squamous cell carcinomas (SCC). The sum of mosaic Eo þ R is false-colored pink to mimic the appearance of eosin, while the AO mosaic is false-colored purple to mimic the appearance of hematoxylin in H&E. In this study, mosaics of 10 Mohs surgical excisions containing invasive SCC, and five containing only normal tissue were subdivided for digital presentation equivalent to 4× histology. Of the total 50 SCC and 25 normal sub-mosaics presented, two reviewers made two and three type-2 errors (false positives), respectively. Limitations to precisely mimic H&E included occasional elastin staining by AO. These results suggest that confocal mosaics may effectively guide staged SCC excisions in skin and other tissues.

show abstract

Section: Discussionmentioning

confidence: 99%

Tri-modal confocal mosaics detect residual invasive squamous cell carcinoma in Mohs surgical excisions

et al. 2012

View full text Add to dashboard Cite

show abstract

“…To achieve these goals, solutions similar to what is widely used on benchtop microscopes can be implemented: multiple excitations in combination with multiple fluorescent markers, targeting multiple biological entities, possibly in interaction. Again, different approaches have been proposed, providing multispectral imaging capabilities, with 23,24 or without 25 microscopic performances.…”

Section: The Need For Multispectral Imagingmentioning

confidence: 99%

Multicolor probe-based confocal laser endomicroscopy: a new world for in vivo and real-time cellular imaging

Vercauteren¹,

Doussoux²,

Cazaux³

et al. 2013

Endoscopic Microscopy VIII

View full text Add to dashboard Cite

Since its inception in the field of in vivo imaging, endomicroscopy through optical fiber bundles, or probe-based Confocal Laser Endomicroscopy (pCLE), has extensively proven the benefit of in situ and real-time examination of living tissues at the microscopic scale. By continuously increasing image quality, reducing invasiveness and improving system ergonomics, Mauna Kea Technologies has turned pCLE not only into an irreplaceable research instrument for small animal imaging, but also into an accurate clinical decision making tool with applications as diverse as gastrointestinal endoscopy, pulmonology and urology.The current implementation of pCLE relies on a single fluorescence spectral band making different sources of in vivo information challenging to distinguish. Extending the pCLE approach to multi-color endomicroscopy therefore appears as a natural plan. Coupling simultaneous multi-laser excitation with minimally invasive, microscopic resolution, thin and flexible optics, allows the fusion of complementary and valuable biological information, thus paving the way to a combination of morphological and functional imaging. This paper will detail the architecture of a new system, Cellvizio Dual Band, capable of video rate in vivo and in situ multi-spectral fluorescence imaging with a microscopic resolution. In its standard configuration, the system simultaneously operates at 488 and 660 nm, where it automatically performs the necessary spectral, photometric and geometric calibrations to provide unambiguously co-registered images in real-time. The main hardware and software features, including calibration procedures and sub-micron registration algorithms, will be presented as well as a panorama of its current applications, illustrated with recent results in the field of pre-clinical imaging.

show abstract

“…In their implementation, a line was scanned across the proximal face of the fiber bundle, and hence ultimately the tissue, and the fluorescent emission was de-scanned, passed through a confocal slit, and then rescanned over a 2D camera to form an image. This system has been developed further [18][19][20], and demonstrated in vivo [21], but the frame rate of 30 fps did not fully realize the potential of line-scanning.…”

Section: Introductionmentioning

confidence: 99%

High speed, line-scanning, fiber bundle fluorescence confocal endomicroscopy for improved mosaicking

Hughes¹,

Yang²

2015

Biomed. Opt. Express

View full text Add to dashboard Cite

A significant limitation of fiber bundle endomicroscopy systems is that the field of view tends to be small, usually only several hundred micrometers in diameter. Image mosaicking techniques can increase the effective image size, but require careful manipulation of the probe to ensure sufficient overlap between adjacent frames. For confocal endomicroscopes, which typically have frame rates on the order of 10 fps, this is particularly challenging. In this paper we demonstrate that line-scanning confocal endomicroscopy can, by use of a high speed linear CCD camera, achieve a frame rate of 120 fps while maintaining sufficient resolution and signal-tonoise ratio to allow imaging of topically stained gastrointestinal tissues. This leads to improved performance of a cross-correlation based mosaicking algorithm when compared with lower frame-rate systems.

show abstract

Multispectral confocal microendoscope for in vivo and in situ imaging

Cited by 53 publications

References 19 publications

Tri-modal confocal mosaics detect residual invasive squamous cell carcinoma in Mohs surgical excisions

Tri-modal confocal mosaics detect residual invasive squamous cell carcinoma in Mohs surgical excisions

Multicolor probe-based confocal laser endomicroscopy: a new world for in vivo and real-time cellular imaging

High speed, line-scanning, fiber bundle fluorescence confocal endomicroscopy for improved mosaicking

Contact Info

Product

Resources

About