Multispectral compressive fluorescence lifetime imaging microscopy with a SPAD array detector

Abstract

Multispectral/hyperspectral Fluorescence Lifetime Imaging Microscopy (λFLIM) is a promising tool for studying functional and structural biological processes. The rich information content provided by a multidimensional dataset is often in contrast with the acquisition speed. In this work, we develop and experimentally demonstrate a wide-field λFLIM setup, based on a novel time-resolved x Single Photon Avalanche Diodes (SPAD) array detector working in a single pixel camera scheme, which parallelizes the spectral…