Multispecies biofilm in an artificial wound bed—A novel model for in vitro assessment of solid antimicrobial dressings

Kučera, Jan; Sojka, Martin; Pavlík, Vojtěch; Szuszkiewicz, Katarzyna Maria; Velebný, Vladimı́r; Klein, Pavel

doi:10.1016/j.mimet.2014.05.008

Cited by 52 publications

(52 citation statements)

References 23 publications

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“…The observations obtained in the present study suggest that, from a clinical point of view, infected wounds associated with Enterococcus faecalis biofilm could be unresponsive to topical honey treatment. In our previous study (Kucera et al, 2014) using the same multispecies bacterial biofilm, we tested two commonly used antimicrobial wound treatments: polyvinylpyrrolidone-iodine complex and cadexomer-iodine complex.…”

Section: Discussionmentioning

confidence: 99%

“…We used the previously described Lubbock chronic wound biofilm model (Sun et al, 2008), modified according to Kucera et al (2014). Briefly, 6 ml medium containing Bolton broth base (Sigma), 1% gelatin, 50% porcine plasma and 5% porcine erythrocytes lysed by freeze-thawing was dispensed into sterile 1.6|10 cm glass tubes.…”

Section: Pcrmentioning

confidence: 99%

“…After treatment, the biofilms were harvested from the artificial wound bed using sterile forceps and a Lang eye spoon, and then homogenized. The number of bacteria per culture was determined using different media as described previously (Kucera et al, 2014) and Brilliance UTI selective medium (Oxoid).…”

Section: Pcrmentioning

confidence: 99%

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Antibiofilm efficacy of honey and bee-derived defensin-1 on multispecies wound biofilm

Sojka

Valachová

Bucekova

et al. 2016

Journal of Medical Microbiology

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Many clinically relevant biofilms are polymicrobial. Examining the effect of antimicrobials in a multispecies biofilm consortium is of great clinical importance. The goal of this study was to investigate the effect of different honey types against bacterial wound pathogens grown in multispecies biofilm and to test the antibiofilm activity of honey defensin-1 (Def-1) in its recombinant form. A modified Lubbock chronic wound biofilm formed by four bacterial species (Staphylococcus aureus, Streptococcus agalactiae, Pseudomonas aeruginosa and Enterococcus faecalis) was used for evaluation of honey and recombinant bee-derived Def-1 antibiofilm efficacy. Recombinant Def-1 was prepared by heterologous expression in Escherichia coli. We showed that different types of honey (manuka and honeydew) were able to significantly reduce the cell viability of wound pathogens (Staphylococcus aureus, Streptococcus agalactiae and Pseudomonas aeruginosa) in mature polymicrobial biofilm. None of the tested honeys showed the ability to eradicate Enterococcus faecalis in biofilm. In addition, recombinant Def-1 successfully reduced the viability of Staphylococcus aureus and Pseudomonas aeruginosa cells within established polymicrobial biofilm after 24 and 48 h of treatment. Interestingly, recombinant Def-1 did not affect the viability of Streptococcus agalactiae cells within the biofilm, whereas both natural honeys significantly reduced the viable bacteria. Although Enterococcus faecalis was highly resistant to Def-1, Def-1 significantly affected the biofilm formation of Enterococcus faecalis and Streptococcus agalactiae after 24 h of treatment, most likely by inhibiting its extracellular polymeric substances production. In conclusion, our study revealed that honey and Def-1 are effective against established multispecies biofilm; however, Enterococcus faecalis grown in multispecies biofilm was resistant to both antimicrobials.

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Section: Discussionmentioning

confidence: 99%

Section: Pcrmentioning

confidence: 99%

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Antibiofilm efficacy of honey and bee-derived defensin-1 on multispecies wound biofilm

Sojka

Valachová

Bucekova

et al. 2016

Journal of Medical Microbiology

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“…; Kucera et al . ), as such the immobilized cell model is provides an alternative that may improve timescales in which candidate dressings are tested at a clinical level (Durante ).…”

Section: Discussionmentioning

confidence: 99%

A systematic comparison of antimicrobial wound dressings using a planktonic cell and an immobilized cell model

et al. 2015

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Aim: The aim of the study was to evaluate the ability of in-vitro planktonic and immobilised cell models for determining the antimicrobial efficacy of common antimicrobial wound dressings.Methods and Results: Five strains of A.baumannii, P.aeruginosa and S. aureus (MRSA) were tested against four antimicrobial wound dressings containing silver, honey or PHMB, Significance and Impact of study: The introduction of planktonic and immobilised cell models as part of testing regimens for wound dressings will provide a more thorough understanding of their antimicrobial and antibiofilm properties.

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“…Approaches typically include the addition of blood components, and/or the incorporation of host tissue substrate components, including artificial skin and collagen [38,[63][64][65][66]. For example, Sun et al developed the Lubbock Chronic Wound Model (LCWM) to mimic the wound environment using a combination of bovine plasma, Bolton Broth and laked horse red blood cells [65].…”

Section: Wound Microcosmmentioning

confidence: 99%

Biofilm Models of Polymicrobial Infection

2015

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Interactions between microbes are complex and play an important role in the pathogenesis of infections. These interactions can range from fierce competition for nutrients and niches to highly evolved cooperative mechanisms between different species that support their mutual growth. An increasing appreciation for these interactions, and desire to uncover the mechanisms that govern them, has resulted in a shift from monomicrobial to polymicrobial biofilm studies in different disease models. Here we provide an overview of biofilm models used to study select polymicrobial infections and highlight the impact that the interactions between microbes within these biofilms have on disease progression. Notable recent advances in the development of polymicrobial biofilm-associated infection models and challenges facing the study of polymicrobial biofilms are addressed. Polymicrobial interactions in biofilmsOver the past 2 decades there has been a revolutionary paradigm shift in the field of microbiology with the appreciation that bacteria present in most biological systems exist in biofilms, rather than in a free-living state. This understanding has dramatically changed the way we study bacteria in the laboratory and resulted in the development of many new experimental systems that replicate biofilm environments [1][2][3][4][5]. Studies utilizing these systems have demonstrated time and time again that bacteria behave very differently when in a biofilm than during planktonic growth. In many ways, we now have to relearn everything we thought we knew about bacterial behavior through the view of the biofilm lens.Similarly, the recent explosion of metagenomic studies has significantly increased our appreciation of the complexity of the microbial populations present in biofilms [6,7]. This is also true for infections, most of which are thought to be biofilm related and inherently polymicrobial, including various species of bacteria, fungi and viruses [8]. It is thought that microbes act in concert to establish biofilms, which in turn can increase tolerance to antimicrobials, exacerbate of the host's immune response and increase persistence at the infection site [7,[9][10][11].Genetic diversity of microbes within biofilm communities is thought to increase the fitness of the residing community, making them more equipped to survive environmental stresses. In large part, this is due to an expanded gene pool, which can be more easily shared within the confines of a biofilm community [12]. Community composition and interactions within the community can have huge influences on bacterial behavior. Thus, just as the behavior of planktonic versus biofilm-associated bacteria is dramatically different, so is that of bacteria in single species versus multispecies systems.Interactions between microbes are complex and highly dependent on context. They can range from fierce competition for nutrients and niches, manifested by antagonistic behavior, to highly evolved cooperative mechanisms between different species that support their mutual grow...

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Multispecies biofilm in an artificial wound bed—A novel model for in vitro assessment of solid antimicrobial dressings

Cited by 52 publications

References 23 publications

Antibiofilm efficacy of honey and bee-derived defensin-1 on multispecies wound biofilm

Antibiofilm efficacy of honey and bee-derived defensin-1 on multispecies wound biofilm

A systematic comparison of antimicrobial wound dressings using a planktonic cell and an immobilized cell model

Biofilm Models of Polymicrobial Infection

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