“…Nevertheless, the task of positioning a single neuron inside each micro-well of the pMEA is not straightforward. Neurons are usually loaded manually using glass micropipettes guided by micromanipulators (Maher et al, 1999;Suzuki et al, 2004;Claverol-Tinture et al, 2007), which is a time-consuming, workintensive procedure. Furthermore, the long period of time required for loading a few micro-wells could result in cells being damaged, which forbids the realisation of large-scale single-cell networks.…”