Multisite Recording of Extracellular Potentials Produced by Microchannel-Confined Neurons In-Vitro

Abstract

Abstract-Towards establishing electrical interfaces with patterned in vitro neurons, we have previously described the fabrication of hybrid elastomer-glass devices polymer-on-multielectrode array technology and obtained single-electrode recordings of extracellular potentials from confined neurons (Claverol-Tinturé et al., 2005). Here, we demonstrate the feasibility of spatially localized multisite recordings from individual microchannel-guided neurites extending from microwell-confined somas with good signal-to-noise ratios (20 dB) and spike magnitudes of up to 300 V.Single-cell current source density (scCSD) analysis of the spatio-temporal patterns of membrane currents along individual processes is illustrated.

Action potential recording from dielectrophoretically positioned neurons inside micro-wells of a planar microelectrode array

“…Nevertheless, the task of positioning a single neuron inside each micro-well of the pMEA is not straightforward. Neurons are usually loaded manually using glass micropipettes guided by micromanipulators (Maher et al, 1999;Suzuki et al, 2004;Claverol-Tinture et al, 2007), which is a time-consuming, workintensive procedure. Furthermore, the long period of time required for loading a few micro-wells could result in cells being damaged, which forbids the realisation of large-scale single-cell networks.…”

Prospects for Neuroprosthetics: Flexible Microelectrode Arrays with Polymer Conductors

“…7b, left) as demonstrated by various groups (Morin et al, 2005;Claverol-Tinture et al, 2007;Benmerah et al, 2009;Lacour et al, 2010). This would increase the likelihood of identifying the actual origin of the bioelectrical signals.…”

Extracellular Potential Recording of Patterned Rat Olfactory Bulb Neuronal Network Using Planar Microelectrode Arrays

“…According to another research, signals collected from a MEA had typical amplitudes in the range of 20-600μV and the thermal noise ranged from 10μV to 20μV [12]. Claverol-Tinture et al [9] measured the extracellular potential of neurons with the open-well configuration. They found that some electrodes, although partially covered by the soma, remained electrically silent because the electrodes were grounded by the culture medium.…”

“…Local cellular environment can be controlled at the micron scale by this approach, which is particularly useful in single-cell-based researches [7]. Techniques with chemically patterned growth substrates [8] or three-dimensional microstructures [9] were used successfully for cell patterning. Bani-Yaghoub et al [10] made specific topographic structures on polydimethylsiloxane (PDMS), and modified the property of PDMS surface with fluoroalkylsilane derivative solution to obtain a patterned neuronal network.…”