Multisite recording of brain field potentials and unit activity in freely moving rats

Buzsáki, György; Bickford, Reginald G.; Ryan, Lawrence J.; Young, Stephanie Z.; Prohaska, O.; Mandel, Ronald J.; Gage, Fred H.

doi:10.1016/0165-0270(89)90038-1

Cited by 66 publications

(27 citation statements)

References 13 publications

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“…Five four-channel MOSF ET input operational amplifiers, mounted in the female connector, served to eliminate cable movement artifacts (Buzsáki et al, 1989). Physiological data were typically recorded as wide band (1 Hz -5 kHz for units and field) or as filtered (1-500 Hz) potentials after amplification (24 channel Grass model 12).…”

Section: Methodsmentioning

confidence: 99%

Cellular–Synaptic Generation of Sleep Spindles, Spike-and-Wave Discharges, and Evoked Thalamocortical Responses in the Neocortex of the Rat

1997

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Thalamocortical neuronal oscillations underlie various field potentials that are expressed in the neocortex, including sleep spindles and high voltage spike-and-wave patterns (HVSs). The mechanism of extracellular current generation in the neocortex was studied in the anesthetized and awake rat. Field potentials and unit activity were recorded simultaneously along trajectories perpendicular to the cortical layers at spatial intervals of 100 m by multiple-site recording silicon probes. Current source density (CSD) analysis revealed that the spatial positions of sinks in layers IV, V-VI, and II-III and of the accompanying sources were similar during sleep spindles, HVSs, and thalamic-evoked responses, although their relative strengths and timings differed. The magnitude and relative timing of the multiple pairs of sinks and sources determined the amplitude variability of HVSs and sleep spindles. The presence of temporally shifted dipoles was also supported by the time distribution of unit discharges in different layers. Putative interneurons discharged with repetitive bursts of 300-500 Hz. The spike component of HVSs was associated with fast field oscillations (400-600 Hz "ripples"). Discharges of pyramidal cells were phase-locked to the ripples. These findings indicate that the major extracellular currents underlying sleep spindles, HVSs, and evoked responses result from activation of intracortical circuitries. We hypothesize that the fast field ripples reflect summed IPSPs in pyramidal cells resulting from the high frequency barrage of interneurons.

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Section: Methodsmentioning

confidence: 99%

Cellular–Synaptic Generation of Sleep Spindles, Spike-and-Wave Discharges, and Evoked Thalamocortical Responses in the Neocortex of the Rat

1997

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“…Five four-channel MOSFET input operational amplifiers, mounted in the cable connector, served to eliminate cable movement artifacts (23). Physiological data were recorded wide-band 0.1 Hz to 5 kHz and sampled at 10 kHz/channel (1 6 channels) with 12-bit precision.…”

Section: Recording and Stimulationmentioning

confidence: 99%

Electrophysiologic Analysis of a Chronic Seizure Model After Unilateral Hippocampal KA Injection

Bragin¹,

Engel²,

Wilson³

et al. 1999

Epilepsia

280

260

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Summary:Purpose: Unilateral intrahippocampal injections of kainic acid (KA) in rats produce spontaneous recurrent limbic seizures and morphologic changes in hippocampus that resemble hippocampal sclerosis in patients with medically refractory mesial temporal lobe epilepsy (MTLE), that form of temporal lobe epilepsy (TLE) associated with hippocampal sclerosis. Interictal in vivo electrophysiologic studies have revealed high-frequency (250-500 Hz) oscillations, termed fast ripples (FRs). These oscillations may uniquely occur in or adjacent to the site of hippocampal KA injection, in areas that generate spontaneous seizures. Similar field potentials also have been demonstrated in the epileptogenic region of patients with TLE. We have now characterized ictal electrographic patterns in this rat model for comparison with those in human TLE and begun to evaluate the role of FRs in the transition to ictus in the KA-treated rat.Methods: Rats received unilateral intrahippocampal injections of KA and, after the development of spontaneous seizures, were implanted with multiple fixed and moveable microelectrodes for single unit, field potential, and EEG recording. They were then monitored by using video-EEG telemetry for several weeks to capture and evaluate electrographic and behavioral seizure types. Results were correlated with Timm's stain demonstration of mossy fiber sprouting.Results: Low-voltage fast (LVF) and hypersynchronous electrographic ictal-onset patterns were seen in the KA-treated rat that resembled similar ictal-onset patterns in patients with TLE. Hypersynchronous, but not LVF, ictal discharges were associated with recurrent FRs. As in the human, hypersynchronous ictal onsets originated predominantly in hippocampus, whereas LVF ictal onsets more often involved extrahippocampal structures. LVF ictal onsets occurred during wakefulness or paradoxical sleep and were usually associated with motor behavior, whereas hypersynchronous ictal onsets occurred during slowwave sleep or periods of immobility and were not associated with motor behavior unless there was transition to another ictal electrographic pattern. Mossy fiber sprouting did not correlate with the frequency of ictal EEG discharges exhibited by each rat but was greater in those rats that demonstrated frequent behavioral seizures. Conclusions:The electrographic features of spontaneous seizures in the KA-treated rat resemble those of patients with medically refractory TLE with respect to EEG pattern ,and localization. Our data suggest that hypersynchronous ictal onsets represent epileptogenic disturbances in hippocampal circuits, whereas LVF ictal onsets may involve extrahippocampal areas having more direct connections to the motor system. Hypersynchronous seizures may involve the same neuronal mechanisms that generate interictal FRs.

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“…During each recording session, one end of a flexible lead terminating in a small operational amplifier circuit (TLC27L7C, Texas Instruments, Dallas TX) (Buzsaki et al, 1989) was connected to the small socket on the bird's head, and the other end was connected to a rotating commutator (H. Adams, C altech Machine Shop, or Radio Shack, Fort Worth, TX). The neural activity signal passed through the commutator to a differential amplifier (A-M Systems), where it was filtered between 300 Hz and 5 kHz.…”

Section: Animals Adult (ͼ125 D Old) Male Zebra Finches (Taeniopyg Iamentioning

confidence: 99%

Singing-Related Neural Activity in a Dorsal Forebrain–Basal Ganglia Circuit of Adult Zebra Finches

1999

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The anterior forebrain pathway (AFP) of songbirds, a specialized dorsal forebrain-basal ganglia circuit, is crucial for song learning but has a less clear function in adults. We report here that neurons in two nuclei of the AFP, the lateral magnocellular nucleus of the anterior neostriatum (LMAN) and Area X, show marked changes in neurophysiological activity before and during singing in adult zebra finches. The presence of modulation before song output suggests that singing-related AFP activity originates, at least in part, in motor control nuclei. Some neurons in LMAN of awake birds also responded selectively to playback of the bird's own song, but neural activity during singing did not completely depend on auditory feedback in the short term, because neither the level nor the pattern of this activity was strongly affected by deafening. The singing-related activity of neurons in AFP nuclei of songbirds is consistent with a role of the AFP in adult singing or song maintenance, possibly related to the function of this circuit during initial song learning.

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Multisite recording of brain field potentials and unit activity in freely moving rats

Cited by 66 publications

References 13 publications

Cellular–Synaptic Generation of Sleep Spindles, Spike-and-Wave Discharges, and Evoked Thalamocortical Responses in the Neocortex of the Rat

Cellular–Synaptic Generation of Sleep Spindles, Spike-and-Wave Discharges, and Evoked Thalamocortical Responses in the Neocortex of the Rat

Electrophysiologic Analysis of a Chronic Seizure Model After Unilateral Hippocampal KA Injection

Singing-Related Neural Activity in a Dorsal Forebrain–Basal Ganglia Circuit of Adult Zebra Finches

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