Multisite Metagenomic Next-Generation Sequencing Improved Diagnostic Performance for Sepsis-Associated Lymphopenia Patients

Li, Dongkai; Gai, Wei; Zhang, Jiahui; Cheng, Wei; Cui, Na; Wang, Hao

doi:10.1128/spectrum.03532-22

Cited by 2 publications

(1 citation statement)

References 31 publications

(34 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, this technique cannot differentiate between MTB, Mycobacterium bovis and BCG [14]. In recent years, mNGS has emerged as a novel technique in pathogen detection [15]. The strengths of mNGS include avoidance of invasive diagnostic procedures, simplification of various infectious diseases evaluations, identification of pathogens otherwise undetected by conventional techniques [16], and contribution to the identification of MTB complex strains as in our study.…”

Section: Discussionmentioning

confidence: 75%

Metagenomic sequencing expedites diagnosis of disseminated BCG in an infant with BRAFV600E mutation

Yang,

Wu,

Wang

et al. 2024

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: Disseminated bacillus Calmette-Guérin (BCG) disease is a rare but serious BCG complication in children. Early diagnosis and timely interventions are essential to improve prognosis. However, its manifestations can closely mimic those of Langerhans cell histiocytosis (LCH), which usually leads to a high rate of misdiagnoses. Herein we report the first case of successful application of biopsy tissue metagenomic next-generation sequencing (mNGS) in the differential diagnosis of disseminated BCG disease and LCH. Case study: A 5-month-old female infant was transferred to our center for the treatment of paroxysmal cough, intermittent hematochezia and trunk rash. Examination on admission showed moderate anemia, erythropenia, thrombocytopenia and hepatosplenomegaly. The immunohistochemistry of her intestinal biopsy samples showed CD1a (+) and Langerin (+). Genetic testing of both peripheral blood and bone marrow samples suggested BRAFV600E mutation. Hence, she was initially diagnosed with LCH. However, no improvement was observed after a course of systemic chemotherapy. The left axillary lymph node and colonic mucosal biopsy specimens were sent for mNGS which resulted in sequence reads of Mycobacterium bovis-BCG. Triple antimycobacterial therapy was started according to the diagnosis. Results: The diagnosis of this case was corrected as disseminated BCG disease by mNGS. Currently, she is doing well clinically and continues to follow-up at our outpatient clinic. Conclusions: This case suggests that mNGS is a valuable tool in the differential diagnosis of disseminated BCG disease and LCH, which can improve the early diagnosis rate of disseminated BCG disease.

show abstract

Section: Discussionmentioning

confidence: 75%

Metagenomic sequencing expedites diagnosis of disseminated BCG in an infant with BRAFV600E mutation

Yang,

Wu,

Wang

et al. 2024

J Infect Dev Ctries

View full text Add to dashboard Cite

show abstract

Metagenomic next-generation sequencing testing from the perspective of clinical benefits

Chen,

Cai,

Wang

et al. 2024

Clinica Chimica Acta

View full text Add to dashboard Cite

Multisite Metagenomic Next-Generation Sequencing Improved Diagnostic Performance for Sepsis-Associated Lymphopenia Patients

Abstract: This study was undertaken to evaluate the reliability of pathogenic diagnoses based on multisite mNGS detection at the clinically suspected sites and to analyze the efficiency of plasma mNGS detection based on lymphocyte subset counts in patients with sepsis-associated lymphopenia.

Cited by 2 publications

References 31 publications

Metagenomic sequencing expedites diagnosis of disseminated BCG in an infant with BRAFV600E mutation

Metagenomic sequencing expedites diagnosis of disseminated BCG in an infant with BRAFV600E mutation

Metagenomic next-generation sequencing testing from the perspective of clinical benefits

Contact Info

Product

Resources

About