Multisite Comparison of Mucociliary and Cough Clearance Measures Using Standardized Methods

Bennett, William D.; Laube, Beth L.; Corcoran, Timothy E.; Zeman, Kirby L.; Sharpless, Gail; Thomas, Kristina; Wu, Jihong; Mogayzel, Peter J.; Pilewski, Joseph M.; Donaldson, Scott H.

doi:10.1089/jamp.2011.0909

Cited by 48 publications

(54 citation statements)

References 25 publications

(55 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Vivo in Subjects with CB MCC measurements by gamma scintigraphy were performed in subjects with CB in the whole, central, and peripheral lung zones using regions of interest (ROI) analyses (31,32). Tracheobronchial (TB) clearance (1-TB retention) was calculated for the first 60 minutes of radiotracer clearance by subtraction of the 24-hour radiotracer retention (33).…”

Section: Measurement Of Mucus Clearancementioning

confidence: 99%

The Relationship of Mucus Concentration (Hydration) to Mucus Osmotic Pressure and Transport in Chronic Bronchitis

Anderson

Coakley

Button

et al. 2015

Am J Respir Crit Care Med

Self Cite

135

154

View full text Add to dashboard Cite

Rationale: Chronic bronchitis (CB) is characterized by persistent cough and sputum production. Studies were performed to test whether mucus hyperconcentration and increased partial osmotic pressure, in part caused by abnormal purine nucleotide regulation of ion transport, contribute to the pathogenesis of CB.Objectives: We tested the hypothesis that CB is characterized by mucus hyperconcentration, increased mucus partial osmotic pressures, and reduced mucus clearance.Methods: We measured in subjects with CB as compared with normal and asymptomatic smoking control subjects indices of mucus concentration (hydration; i.e., percentage solids) and sputum adenine nucleotide/nucleoside concentrations. In addition, sputum partial osmotic pressures and mucus transport rates were measured in subjects with CB.Measurements and Results: CB secretions were hyperconcentrated as indexed by an increase in percentage solids and total mucins, in part reflecting decreased extracellular nucleotide/nucleoside concentrations. CB mucus generated concentration-dependent increases in partial osmotic pressures into ranges predicted to reduce mucus transport. Mucociliary clearance (MCC) in subjects with CB was negatively correlated with mucus concentration (percentage solids). As a test of relationships between mucus concentration and disease, mucus concentrations and MCC were compared with FEV 1 , and both were significantly correlated.Conclusions: Abnormal regulation of airway surface hydration may slow MCC in CB and contribute to disease pathogenesis.

show abstract

Section: Measurement Of Mucus Clearancementioning

confidence: 99%

The Relationship of Mucus Concentration (Hydration) to Mucus Osmotic Pressure and Transport in Chronic Bronchitis

Anderson

Coakley

Button

et al. 2015

Am J Respir Crit Care Med

Self Cite

135

154

View full text Add to dashboard Cite

show abstract

“…While many studies have investigated the regulation of the individual components of MCC in vitro, there have been fewer investigations of the integrated system, largely because of the difficulty of performing MCC studies in vivo. The most common and least invasive technique for measuring MCC in vivo involves measuring the disappearance of radioactive tracers from the airways (2). This technique has been utilized to study MCC in mice (3,10), dogs (23), and sheep (35), as well as in humans (2).…”

mentioning

confidence: 99%

“…The most common and least invasive technique for measuring MCC in vivo involves measuring the disappearance of radioactive tracers from the airways (2). This technique has been utilized to study MCC in mice (3,10), dogs (23), and sheep (35), as well as in humans (2). An alternative method to study mucociliary transport (MCT) in vivo records the movement of individual radiopaque disks (20,35).…”

mentioning

confidence: 99%

Continuous mucociliary transport by primary human airway epithelial cells in vitro

Sears

Yin

Ostrowski

2015

American Journal of Physiology-Lung Cellular and Molecular Phys

View full text Add to dashboard Cite

show abstract

“…PCD and control groups were similar in age and sex distribution but PCD patients had impaired lung function (mean FEV1 74% (range 36-105%) predicted in PCD patients and 97% (range 93-100%) predicted in controls) (table 1). Participants inhaled nebulised technetium-99m labelled nanocoll (GE Healthcare, Little Chalfont, UK) via a DeVilbiss 646 nebuliser (DeVilbiss Healthcare, Somerset, PA, USA) while breathing to ensure the best deposition in the central airways, as described previously [11]. Planar gamma camera images were taken at time 0, 1, 2, 4 and 24 h on a dual-head GE Infinia gamma camera (GE Systems, Milwaukee, WI, USA).…”

mentioning

confidence: 99%

Pulmonary radioaerosol mucociliary clearance in primary ciliary dyskinesia

et al. 2014

View full text Add to dashboard Cite

Primary ciliary dyskinesia (PCD) is a genetic condition affecting one in 10 000-40 000 people from birth [1]; cilia fail to beat, and the airway clearance of mucus and debris is severely impaired. If untreated, this results in progressive lung infection leading to bronchiectasis and ultimately respiratory failure. Additionally, delayed diagnosis has implications for genetic counselling, appropriate management of glue ear and fertility advice. Early diagnosis and appropriate treatment are believed to improve outcome. The diagnosis of PCD is highly specialised and results can remain inconclusive, despite state of the art equipment and diagnostic techniques. A European consensus statement [2,3] highlighted that there is no ''gold-standard'' diagnostic test; diagnosis requires expert review of clinical history and screening tests (nasal nitric oxide measurement) alongside analysis of ciliary function and ultrastructure [2]. It is recommended that ciliary activity of respiratory epithelial cells obtained by nasal or bronchial brushing is recorded using a high-speed video camera mounted on a microscope. The images are played back in slow motion to analyse ciliary beat pattern (CBP) and frequency (CBF). Transmission electron microscopy (TEM) is used to assess ciliary ultrastructure [4]. Diagnostic uncertainty can be caused by secondary damage of the epithelium during sampling or due to infection or inflammation of epithelia; this damage can lead to abnormalities of ultrastructure, CBF and CBP. Furthermore, diagnosis is hindered by normal ciliary ultrastructure in 3-30% of cases of PCD [5,6]. To improve diagnostic certainty, a variety of further investigations can be employed [2], including reanalysis of CBF, CBP and TEM following culture of the cells at an air-liquid interface [7,8], or using immunofluorescence microscopy [9] to identify ciliary proteins. A single-centre study has previously reported the use of pulmonary radioaerosol mucociliary clearance (MCC) in the diagnosis of PCD [10]. The method is based on clearance patterns after the inhalation of a radioaerosol tracer. It provides a whole-lung functional test for pulmonary radioaerosol MCC. The investigation is noninvasive and has been used in thousands of patients with other lung diseases, as young as ,5 years. The authors reported that MCC was an effective noninvasive functional test for PCD [10] but the study was preliminary, and the feasibility of this complex technique and interpretation of data have not been assessed in other centres. We therefore conducted a study to replicate the results using a standardised protocol. This study was approved by the National Research Ethics Service (South Central committee 11/ SC/0192) and all subjects gave written informed consent.

show abstract

Multisite Comparison of Mucociliary and Cough Clearance Measures Using Standardized Methods

Abstract: This standardized protocol may prove beneficial in multicenter trials for testing new therapies that are designed to improve MCC/CC.

Cited by 48 publications

References 25 publications

The Relationship of Mucus Concentration (Hydration) to Mucus Osmotic Pressure and Transport in Chronic Bronchitis

The Relationship of Mucus Concentration (Hydration) to Mucus Osmotic Pressure and Transport in Chronic Bronchitis

Continuous mucociliary transport by primary human airway epithelial cells in vitro

Pulmonary radioaerosol mucociliary clearance in primary ciliary dyskinesia

Contact Info

Product

Resources

About