Multiscattering-enhanced absorbance to enable the sensitive analysis of extremely diluted biological samples: Determination of oxidative potential in exhaled air

Goekce, Sami; Concha-Lozano, Nicolas; Sauvain, Jean‐Jacques; Hemmendinger, Maud; Portela, Alejandro; Sergent, Elise; Andujar, Pascal; Pairon, Jean‐Claude; Wild, Pascal; Suárez, Guillaume

doi:10.1016/j.medntd.2022.100120

Cited by 4 publications

(6 citation statements)

References 18 publications

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“…We found that OPEA was significantly higher among adults who had experienced a SARS-CoV-2 infection compared to SARS-CoV-2 seronegative adults on one hand and among adults with an obstructive syndrome compared to the adults with normal FEV1/FVC value on the other. These findings confirm the results of the pilot clinical study, where OPEA was found to discriminate the COPD patients from those free of COPD [ 6 ], while a clinical COPD diagnosis was based on the GOLD criteria [ 17 ]. Furthermore, we found that OPEA is stable with respect to individual characteristics such as sex and age, but also with respect to some exposures such as tobacco smoking or different types of diets.…”

Section: Discussionsupporting

confidence: 86%

“…Furthermore, we found that OPEA is stable with respect to individual characteristics such as sex and age, but also with respect to some exposures such as tobacco smoking or different types of diets. Such a stability was also observed in the pilot clinical study [ 6 ] and in two ongoing studies [ 26 , 27 ], and is a valuable characteristic for a candidate biomarker, facilitating its interpretation and therefore its use in clinical practice.…”

Section: Discussionmentioning

confidence: 55%

“…Once placed in the optical cell cavity, all oxidants in the sample are quantitatively determined as the measured absorbance (580 nm) varies linearly with the amount of H 2 O 2 equivalents (pmol). The calibration of the OPEA analyzer with H 2 O 2 shows the efficiency of the sensing strategy with an extremely low limit-of-detection achieved below 3 pmol compared to a LOD > 200 pmol with the standard cuvette-spectrometer configuration [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…To evaluate the OPEA analyzer and OPEA significance with respect to chronic obstructive pulmonary disease (COPD), a pilot clinical study was previously conducted in France at the Department of Pneumology and Occupational Pathology of an Intercommunal Hospital Center in Créteil. This study included 53 COPD and healthy patients and showed that OPEA allows discriminating patients with and without COPD, particularly in non-smokers [ 6 ]. Moreover, a statistically significant correlation was found (Pearson coefficient: −0.33) between the calculated OPEA and the FEV1/FVC ratio, which is decreased in COPD patients, indicating the degree of bronchiolar obstruction [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…This study included 53 COPD and healthy patients and showed that OPEA allows discriminating patients with and without COPD, particularly in non-smokers [ 6 ]. Moreover, a statistically significant correlation was found (Pearson coefficient: −0.33) between the calculated OPEA and the FEV1/FVC ratio, which is decreased in COPD patients, indicating the degree of bronchiolar obstruction [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

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Oxidative Potential in Exhaled Air (OPEA) as a Tool for Predicting Certain Respiratory Disorders in the General Adult Population: Cross-Sectional Analysis Nested in the Swiss Health Study

et al. 2022

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In a pilot clinical study, OPEA allowed for distinguishing participants with and without chronic obstructive pulmonary disease. This study aimed to assess whether abnormal spirometry parameters and immunity against SARS-CoV-2 are associated with increased OPEA and estimating the OPEA reference interval. Swiss adult residents of the Vaud Canton aged 20–69 years randomly selected from the Federal Statistical Office’s registries, speaking French or German, were included and examined between 1 October 2020 and 31 December 2021. General health status and presence of respiratory diseases were assessed by questionnaire and spirometry. Spirometric results were compared with the predicted values and their lower limits of norms of the Global Lung Function Initiative. SARS-CoV-2-seroprevalence was assessed using the Luminex-based test of IgG. Statistical analysis consisted of unilateral t-tests and ANOVA. Lower and upper limit of OPEA reference interval with associated 90%-confidence interval (90%CI) were estimated for the sub-sample of healthy adults by bootstrap, after excluding outliers. The study sample included 247 participants. SARS-CoV-2-seropositive participants and those with an obstructive syndrome had a significantly higher OPEA than seronegative and healthy participants. The estimated reference interval was: −0.0516 (90%CI = −0.0735; −0.0316); −0.0044 (90%CI = −0.0224; 0.0153). OPEA could predict inflammatory-based respiratory disorders, but needs further validation in different settings and for other pathologies.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Oxidative Potential in Exhaled Air (OPEA) as a Tool for Predicting Certain Respiratory Disorders in the General Adult Population: Cross-Sectional Analysis Nested in the Swiss Health Study

et al. 2022

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Metal and oxidative potential exposure through particle inhalation and oxidative stress biomarkers: a 2-week pilot prospective study among Parisian subway workers

Sauvain,

Hemmendinger,

Charreau

et al. 2024

Int Arch Occup Environ Health

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Objective In this pilot study on subway workers, we explored the relationships between particle exposure and oxidative stress biomarkers in exhaled breath condensate (EBC) and urine to identify the most relevant biomarkers for a large-scale study in this field. Methods We constructed a comprehensive occupational exposure assessment among subway workers in three distinct jobs over 10 working days, measuring daily concentrations of particulate matter (PM), their metal content and oxidative potential (OP). Individual pre- and post-shift EBC and urine samples were collected daily. Three oxidative stress biomarkers were measured in these matrices: malondialdehyde (MDA), 8-hydroxy-2′deoxyguanosine (8-OHdG) and 8-isoprostane. The association between each effect biomarker and exposure variables was estimated by multivariable multilevel mixed-effect models with and without lag times. Results The OP was positively associated with Fe and Mn, but not associated with any effect biomarkers. Concentration changes of effect biomarkers in EBC and urine were associated with transition metals in PM (Cu and Zn) and furthermore with specific metals in EBC (Ba, Co, Cr and Mn) and in urine (Ba, Cu, Co, Mo, Ni, Ti and Zn). The direction of these associations was both metal- and time-dependent. Associations between Cu or Zn and MDAEBC generally reached statistical significance after a delayed time of 12 or 24 h after exposure. Changes in metal concentrations in EBC and urine were associated with MDA and 8-OHdG concentrations the same day. Conclusion Associations between MDA in both EBC and urine gave opposite response for subway particles containing Zn versus Cu. This diverting Zn and Cu pattern was also observed for 8-OHdG and urinary concentrations of these two metals. Overall, MDA and 8-OHdG responses were sensitive for same-day metal exposures in both matrices. We recommend MDA and 8-OHdG in large field studies to account for oxidative stress originating from metals in inhaled particulate matter.

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Occupational exposure to nanomaterials and biomarkers in exhaled air and urine: Insights from the NanoExplore international cohort

Hemmendinger,

Squillacioti,

Charreau

et al. 2023

Environment International

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Multiscattering-enhanced absorbance to enable the sensitive analysis of extremely diluted biological samples: Determination of oxidative potential in exhaled air

Cited by 4 publications

References 18 publications

Oxidative Potential in Exhaled Air (OPEA) as a Tool for Predicting Certain Respiratory Disorders in the General Adult Population: Cross-Sectional Analysis Nested in the Swiss Health Study

Oxidative Potential in Exhaled Air (OPEA) as a Tool for Predicting Certain Respiratory Disorders in the General Adult Population: Cross-Sectional Analysis Nested in the Swiss Health Study

Metal and oxidative potential exposure through particle inhalation and oxidative stress biomarkers: a 2-week pilot prospective study among Parisian subway workers

Occupational exposure to nanomaterials and biomarkers in exhaled air and urine: Insights from the NanoExplore international cohort

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