Multiscale Origami Structures as Interface for Cells

Angelin, Alessandro; Weigel, Simone; Garrecht, Ruben; Meyer, Rebecca; Bauer, J.; Kumar, Ravi; Hirtz, Michael; Niemeyer, Christof M.

doi:10.1002/anie.201509772

Cited by 92 publications

(102 citation statements)

References 61 publications

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“…Moreover, the cost of synthetic DNA used for these purposes has been reduced in recent times, and custom‐made DNA origami scaffolds provide purpose‐specific nanostructures . One such advancement is the recently developed origami‐based structures that provide an interface for presenting specifically patterned ligands to cells . Origami‐based structures allow the study of biomolecular interaction which can be incorporated into future sensor fabrication.…”

Section: Resultsmentioning

confidence: 99%

Nucleic Acid Nanostructures for Chemical and Biological Sensing

Chandrasekaran

Wady

Subramanian

2016

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The nanoscale features of DNA have made it a useful molecule for bottom-up construction of nanomaterials, for example, two- and three-dimensional lattices, nanomachines, and nanodevices. One of the emerging applications of such DNA-based nanostructures is in chemical and biological sensing, where they have proven to be cost-effective, sensitive and have shown promise as point-of-care diagnostic tools. DNA is an ideal molecule for sensing not only because of its specificity but also because it is robust and can function under a broad range of biologically relevant temperatures and conditions. DNA nanostructure-based sensors provide biocompatibility and highly specific detection based on the molecular recognition properties of DNA. They can be used for the detection of single nucleotide polymorphism and to sense pH both in solution and in cells. They have also been used to detect clinically relevant tumor biomarkers. In this review, recent advances in DNA-based biosensors for pH, nucleic acids, tumor biomarkers and cancer cell detection are introduced. Some challenges that lie ahead for such biosensors to effectively compete with established technologies are also discussed.

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Section: Resultsmentioning

confidence: 99%

Nucleic Acid Nanostructures for Chemical and Biological Sensing

Chandrasekaran

Wady

Subramanian

2016

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“…However, these 3D structures are more stable than mechanically sensitive 2D structures and the binding of the proteins was only achieved by hybridization of previously synthesized oligonucleotide–protein conjugates. Since direct ligation of proteins on the surface of DONs brings advantages in terms of synthesis effort and costs, and since flexible 2D DONs are important reagents for surface‐based cell assays, there is still a great need for a convenient and robust method to prepare and purify fragile DONs on which proteins have been immobilized by chemoselective coupling methods.…”

Section: Figurementioning

confidence: 99%

“…This limitation could be overcome by alternative bead‐binding/release systems, for example, based on DNA hybridization and strand displacement mechanisms . Even in the current version, the method is of great value for the further development of the field, as it enables new ligation methods to be evaluated on mechanically flexible and fragile origami structures, which have proven their utility for surface‐based cell assays and fundamental research in biocatalysis …”

Section: Figurementioning

confidence: 99%

Solid‐Phase Synthesis and Purification of Protein–DNA Origami Nanostructures

Burgahn

Garrecht

Rabe

et al. 2019

Chemistry A European J

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We present a facile method for the combined synthesis and purification of protein‐decorated DNA origami nanostructures (DONs). DONs bearing reductively cleavable biotin groups in addition to ligands for ligation of recombinant proteins are bound to magnetic beads. Protein immobilization is conducted with a large protein excess to achieve high ligation yields. Subsequent to cleavage from the solid support, pure sample solutions are obtained which are suitable for direct AFM analysis of occupation patterns. We demonstrate the method's utility using three different orthogonal ligation methods, the “halo‐based oligonucleotide binder” (HOB), a variant of Halo‐tag, the “SpyTag/SpyCatcher” (ST/SC) system, and the enzymatic “ybbR tag” coupling. We find surprisingly low efficiency for ST/SC ligation, presumably due to electrostatic repulsion and steric hindrance, whereas the ybbR method, despite its ternary nature, shows good ligation yields. Our method is particularly useful for the development of novel ligation methods and the synthesis of mechanically fragile DONs that present protein patterns for surface‐based cell assays.

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“…[48].C opyright Elsevier,2008. [66] TheM OSAIC platform can be used to investigate fundamental principles of cell signaling.C ellular membrane receptors are frequently geometrically constrained, and they often organize in supramolecular assemblies before or as part of their response to ligand binding. [50].C opyright MDPI AG, 2015.…”

Section: Dna Chips For Cell Cultivationmentioning

confidence: 99%

DNA Surface Technology: From Gene Sensors to Integrated Systems for Life and Materials Sciences

Schneider

Niemeyer

2018

Angewandte Chemie

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The evolution of DNA microarray technology has led to sophisticated DNA chips that are being used as routine tools for fundamental and applied genome research such as genotyping and expression profiling. Owing to their capability for highly parallel, site‐directed immobilization of complementary nucleic acids through canonical Watson–Crick base‐pairing, however, DNA‐modified surfaces can also be used for the assembly of complex surface architectures comprised of non‐nucleic acid compounds, such as proteins or colloidal materials. Furthermore, implementation of functional DNA devices and structural DNA nanotechnology can unlock the full potential of DNA surfaces. Based on case studies from diagnostics, sensing, proteome research, cell adhesion, and cell signaling, we show how classical gene sensors have developed into modern integrated systems and platforms for various applications in life sciences and materials research.

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Multiscale Origami Structures as Interface for Cells

Cited by 92 publications

References 61 publications

Nucleic Acid Nanostructures for Chemical and Biological Sensing

Nucleic Acid Nanostructures for Chemical and Biological Sensing

Solid‐Phase Synthesis and Purification of Protein–DNA Origami Nanostructures

DNA Surface Technology: From Gene Sensors to Integrated Systems for Life and Materials Sciences

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