Multiscale modelling of chromatin 4D organization in SARS-CoV-2 infected cells

Abstract

SARS-CoV-2 can re-structure chromatin organization and alter the epigenomic landscape of the host genome, but the mechanisms that produce such changes remain unclear. Here, we use polymer physics to investigate how the chromatin of the host genome is re-organized upon infection with SARS-CoV-2. We show that re-structuring of A/B compartments can be explained by a re-modulation of intra-compartment homo-typic affinities, which leads to the weakening of A-A interactions and the enhancement of A-B mixing. At the TAD level, re-arrangements are physically described by a reduction in the loop extrusion activity coupled with an alteration of chromatin phase-separation properties, resulting in more intermingling between different TADs and a spread in space of the TADs themselves. In addition, the architecture of loci relevant to the antiviral interferon response, such as DDX58 or IFIT, becomes more variable within the 3D single-molecule population of the infected model, suggesting that viral infection leads to a loss of chromatin structural specificity. Analysing the time trajectories of pairwise gene-enhancer and higher-order contacts reveals that this variability derives from increased fluctuations in the chromatin dynamics of infected cells. This suggests that SARS-CoV-2 alters gene regulation by impacting the stability of the contact network in time.

Polymer physics models reveal structural folding features of single-molecule gene chromatin conformations

“…On the other hand, theoretical models of chromatin have been instrumental in bolstering experimental technologies to understand the 3D organization of the genome [37]. These models, including polymer-physics-based and computational approaches , have been used, e.g., to dissect the fundamental molecular mechanisms shaping DNA contact formation and to derive quantitative testable predictions particularly in disease contexts [81][82][83][84].…”