Multiresistance, beta-lactamase-encoding genes and bacterial diversity in hospital wastewater in Rio de Janeiro, Brazil

Chagas, Thiago Pavoni Gomes; Seki, Liliane Miyuki; Cury, José; Oliveira, Joana; Dávila, Alberto M. R.; Silva, D.M.; Asensi, Marise Dutra

doi:10.1111/j.1365-2672.2011.05072.x

Cited by 149 publications

(104 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…There are five main clusters of CTX-M betalactamases: CTX-M-1, CTX-M-2, CTX-M-8, CTX-M-9, and CTX-M-25, and except for the last one, all of them have already been described in strains from Brazil. The most frequent encoding genes reported in our region are bla CTX-M-15 [6,12] from the CTX-M-1 cluster, bla CTX-M-2 [6][7][8][9][10][11][12][13][14] and bla CTX-M-59 [5,13] from the CTX-M-2 cluster, bla CTX-M-8 [8] from the CTX-M-8 cluster, and bla CTX-M-9 [10,[12][13] from the CTX-M-9 cluster. The reason for the spread of CTX-M lies in their ability to be mobilized by genetic structures, especially insertion sequences and integrons.…”

Section: Introductionmentioning

confidence: 77%

“…In Brazil, there is no report of TEM enzymes that express an ESBL phenotype, while some SHV ESBLs are frequently reported (SHV-5, SHV-12) [5][6]. CTX-M enzymes are spread worldwide, and in Latin America, they are considered endemic and play an important role in the resistance landscape in Brazil, where several reports are continuously being published [5][6][7][8][9][10][11][12][13][14]. There are five main clusters of CTX-M betalactamases: CTX-M-1, CTX-M-2, CTX-M-8, CTX-M-9, and CTX-M-25, and except for the last one, all of them have already been described in strains from Brazil.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Complex class 1 integrons harboring CTX-M-2-encoding genes in clinical Enterobacteriaceae from a hospital in Brazil

Dropa

Balsalobre

Lincopán

et al. 2015

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: CTX-M enzymes are the most prevalent extended-spectrum beta-lactamases (ESBLs) in Brazil and around the world. The spread of CTX-M lies in their ability to be mobilized by insertion sequences and integrons. This study aimed to identify the mobile genetic structures associated with bla CTX-M genes from clinical Enterobacteriaceae strains. Methodology: Twenty-eight clinical non-clonal Enterobacteriaceae were screened by PCR for the presence of bla CTX-M genes and class 1 integrase (int1), and for the association of bla CTX-M with class 1 integrons. Plasmid incompatibility groups were assessed by PBRT. Wild-type plasmids were transformed into electrocompetent E. coli, and the S1-PFGE technique was used to verify the presence of high-molecularweight plasmids in both wild-type strains and E. coli transformants. Results: Sequencing showed that strains carried bla CTX-M-2 (n = 25) and bla CTX-M-59 (n = 3) genes inserted into the 3'-end of complex class 1 integrons. Thirteen strains also carried bla TEM and bla SHV genes. CTX-M-2/59-containing complex class 1 integrons were also present in E. coli transformants. The most frequent Inc groups were IncA/C (n = 10) and IncF (n = 8). Heavy plasmids were observed in both wild-type strains and E. coli transformants. Conclusions: The presence of the same bla CTX-M-2-group -containing genetic structure in seven Enterobacteriaceae species isolated at seven hospital wards shows the great mobility potential of complex class 1 integrons. Also, this is the first report of TEM-15, SHV-45, and SHV-55 in Latin America. The genetic environment of bla CTX-M-2 accounts for their maintenance and spread among Gram-negative bacteria.

show abstract

Section: Introductionmentioning

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Complex class 1 integrons harboring CTX-M-2-encoding genes in clinical Enterobacteriaceae from a hospital in Brazil

Dropa

Balsalobre

Lincopán

et al. 2015

J Infect Dev Ctries

View full text Add to dashboard Cite

show abstract

“…Water certainly promotes the circulation of Enterobacteriaceae strains (be they resistant or not). [18][19][20][21][22][23][24][25] Moreover, access to drinking water is not properly insured in many parts of the city. As such, Enterobacteriaceae from stools can easily affect many people through contaminated water.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of CTX-M beta-Lactamases in Escherichia coli from community-acquired urinary tract infections and associated risk factors among women in Cameroon

Djuikoue

Njajou

Kamga

et al. 2017

JER

View full text Add to dashboard Cite

Background: In recent years, a worldwide dissemination of CTX-M beta-lactamase-type in Escherichia coli strains isolates from community-acquired urinary tract infections (CA-UTI) has been observed. However little is known on the prevalence and risk factors of this global threat in developing countries. Objective: The aim of this study was to study the prevalence and risk factors for CA-UTI in Yaoundé, Cameroon. Methods: Eighty six patients with urinary E.coli infection recruited from 10 health structures in the town of Yaoundé, Cameroon. After taking the first urine, faeces were collected from the patients for the study of the intestinal flora. The sample collection of faeces was done on a selected gel of enterobacteria resistant to third generation of cephalosporin. The molecular typing of extended-spectrum β-lactamase (ESBL) was carried out. Results: Eighty-six strains of E. coli from 86 patients were included. We found that 39 (45.3%) strains produced an extendedspectrum beta-lactamase. Among risk factors, previous use of antibiotic and the dry season were associated with the presence of an ESBL-producing strain in the urine. All ESBL were identified as CTX-M. The production of CTX-M was found to be significantly associated with resistance to fluoroquinolones, aminoglycosides and to the association of trimethoprim-sulfamethazole. Conclusions: The prevalence of CTX-M ESBL in Yaoundé, Cameroon, provides new evidence on the global dissemination of CTX-M and the extent of this phenomenon in developing countries.

show abstract

“…This was supported by Study conducted in Switzerland which showed that increased proportions of highly and extremely multi-resistant bacteria among the isolated sulfamethoxazole/ trimethoprim and streptomycin resistant strains in the sample of treated wastewater compared to the wastewater treatment plant inlet sample [34]. Study conducted in Australia [35], Brazil [36] and China [37] that investigates a hospital sewage treatment system also showed, the treatment of the hospital wastewater may not be totally effective in removing multiple drug resistant bacteria and …”

mentioning

confidence: 94%

Antibiotic Resistant Bacteria from Treated and Untreated Hospital Wastewater at Ayder Referral Hospital, Mekelle, North Ethiopia

Asfaw¹,

Negash²,

Kahsay³

et al. 2017

AiM

View full text Add to dashboard Cite

The widespread emergence of antibiotic resistance among bacterial pathogens has become one of the most serious challenges in Ethiopia. This study determined the prevalence and drug resistance patterns of bacterial pathogens isolated from treated and untreated wastewater released from Ayder Referral Hospital in Northern Ethiopia. A cross sectional study design was conducted from September-December, 2015 in wastewater released from Ayder referral hospital. A total of 40 composite samples were aseptically collected, transported and processed for enumeration of indicator organisms, bacteriological identification and susceptibility testing following standard procedure. Data obtained were entered and analyzed using SPSS version 20. Mean heterotrophic plate count, total coliform count, fecal coliform count and E. coli count were found to be 1.6 × 10 6 CFU/mL, 2.2 × 10 6 CFU/100 mL, 2.0 × 10 5 CFU/100 mL and 1.1 × 10 4 CFU/100 mL from treated wastewater respectively.

show abstract

Multiresistance, beta-lactamase-encoding genes and bacterial diversity in hospital wastewater in Rio de Janeiro, Brazil

Cited by 149 publications

References 49 publications

Complex class 1 integrons harboring CTX-M-2-encoding genes in clinical Enterobacteriaceae from a hospital in Brazil

Complex class 1 integrons harboring CTX-M-2-encoding genes in clinical Enterobacteriaceae from a hospital in Brazil

Prevalence of CTX-M beta-Lactamases in Escherichia coli from community-acquired urinary tract infections and associated risk factors among women in Cameroon

Antibiotic Resistant Bacteria from Treated and Untreated Hospital Wastewater at Ayder Referral Hospital, Mekelle, North Ethiopia

Contact Info

Product

Resources

About