Multiresidue Method for Confirmation of Macrolide Antibiotics in Bovine Muscle by Liquid Chromatography/Mass Spectrometry

Abstract

A particle beam/liquid chromatographic/mass spectrometric (PB/LC/MS) method capable of determining 5 macrolides in bovine muscle is described. Spiramycin, tylosin, tilmicosin, erythromycin, and josamycin residues in bovine muscle are confirmed by reversed-phase LC/MS incorporating gradient elution. Macrolides are extracted from tissue with chloroform, and the extract is purified with a diol solid-phase extraction cleanup. The 5 macrolides are identified by negative and positive chemical ionization with selective ion monitoring of 2 ions in each mode. The procedure confirms the presence of each macrolide at 50 μg/kg in spiked samples.

Analysis of a commercial preparation of erythromycin estolates by tandem mass spectrometry and high performance liquid chromatography/electrospray ionization tandem mass spectrometry using an ion trap mass spectrometer

“…Because of its low detection limit, high specificity and, more importantly, abundant structure information, mass spectrometry (MS) has been considered one of the ideal devices for drug analysis. Analysis of erythromycins by various conventional MS techniques have been reported 7–11. Recently, electrospray ionization has rapidly emerged as a very promising technique for the analysis of compounds with medium or high polarity.…”

“…Recently, ESI‐MS/MS and HPLC/ESI‐MS/MS have been applied to the analysis of various erythromycins 8,. 10–12 These reports mainly focused on the analysis of the major erythromycin8,, 11 and its decomposition products 12. In this report, the major component, erythromycin estolate A, as well as related minor substances, in a commercial preparation, were studied by MS, MS/MS and HPLC/MS/MS.…”

Analysis of erythromycin and tylosin in bovine muscle using disposable screen printed electrodes

“…ELISA. Extraction of spiked and real tissue sample was carried out by modifying the procedure of Delépine et al; 13 the scheme is shown in Fig. (1).…”

“…Gas chromatography and liquid chromatography coupled to mass spectrometry, on account of their high specificity based on the structural information of the analytes, have also been reported as useful confirmatory methods for the determination of macrolides in animal tissues. [11][12][13][14] However, the analysis of these compounds with chromatographic methods is time consuming and equipment costs are high.…”

Determination of tylosin residues in pig tissues using high-performance liquid chromatography

“…EU legislation has established 1 a maximum residue limit (MRL) of 100 mg kg 21 for porcine edible tissues. Currently available methods [2][3][4][5] for the detection of tylosin at the MRL level are either laborious or require significant volumes of solvents (often chlorinated solvents), and are generally suitable for only one or two tissues. Hence for control purposes a reliable and simple method for the determination of tylosin in edible pig tissues is needed.…”