Multiresidue determination of (fluoro)quinolone antibiotics in swine kidney using liquid chromatography–tandem mass spectrometry

Vyncht, Géry Van; Amaya, Janosi; Bordin, Guy; Toussaint, B.; Maghuin‐Rogister, Guy; Pauw, Edwin De; Rodríguez, A. R.

doi:10.1016/s0021-9673(02)00092-4

Cited by 78 publications

(38 citation statements)

References 21 publications

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“…CID was carried out, on the ion of interest, by collisions with the helium background gas present in the trap for 40 ms. Product ion mass spectra (MS 3 [30][31][32][33][34]. According to the EC Decision [5], using QIT, each precursor-product ion transition earns 1.5 IPs.…”

Section: Optimization Of Ce-ms Conditionsmentioning

confidence: 99%

Determination of quinolone residues in chicken and fish by capillary electrophoresis‐mass spectrometry

2006

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A specific pressure-assisted CE-MS method is described for the analysis of five quinolone residues. MS using a single quadrupole is compared with multiple-stage MS using a quadrupole IT (QIT-MS(n)). The procedure involves a common sample preparation by SPE on disposable cartridges. The most suitable electrolyte is 60 mM (NH(4))(2)CO(3) at pH 9.2. Single quadrupole does not provide enough fragmentation to confirm identities according to the current legislation. However, QIT-MS(n) achieves selective fragmentation. Using this method, danofloxacin, enrofloxacin, flumequine, ofloxacin, and pipemidic acid are analyzed in fortified samples of chicken and fish. Recoveries at levels of 50 ng/g were 62-99%, except for flumequine, which gives recoveries > or =45%. RSDs are from 9 to 16% and the LOD is equal (20 ng/g) for the five analytes. Confirmation of the quinolones' identity is achieved using QIT-MS(3). Forty samples of chicken and fish taken from different local markets are analyzed. Enrofloxacin is also determined in incurred chicken muscle using this method.

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Section: Optimization Of Ce-ms Conditionsmentioning

confidence: 99%

Determination of quinolone residues in chicken and fish by capillary electrophoresis‐mass spectrometry

2006

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“…However, these methods are complicated, timeconsuming, and specificity-lacking (Kurittu et al, 2000). Instrumental methods, including liquid chromatography-mass spectrometry (LC-MS) and highperformance liquid chromatography (HPLC) (Johnston et al, 2002;van Vyncht et al, 2002;EspinosaMansilla et al, 2005;Toussaint et al, 2005a;2005b;Zeng et al, 2005), are the most current widely used methods for CPFX residues detection. These methods are sensitive and highly specific, but they require expensive equipment, large volumes of solvents, and highly trained individuals for operating complicated instruments, with time-consuming sample cleanup processes.…”

Section: Introductionmentioning

confidence: 99%

Preparation of high-affinity rabbit monoclonal antibodies for ciprofloxacin and development of an indirect competitive ELISA for residues in milk

Huang

Yin

Lü

et al. 2010

J. Zhejiang Univ. Sci. B

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Abstract:A convenient competitive enzyme-linked immunosorbent assay (ELISA) for ciprofloxacin (CPFX) was developed by using rabbit monoclonal antibodies (RabMAbs) against a hapten-protein conjugate of CPFX-bovine serum albumin (BSA). The indirect competitive ELISA of CPFX had a concentration at 50% inhibition (IC 50 ) of 1.47 ng/ml and a limit of detection (LOD) of 0.095 ng/ml. The mAb exhibited some cross-reactivity, however, not so high with enrofloxacin (28.8%), ofloxacin (13.1%), norfloxacin (11.0%), fleroxacin (22.6%), and pefloxacin (20.4%). And it showed almost no cross-reactivity with other antibiotics or sulfonamides evaluated in this study. The competitive ELISA kit developed here could be used as a screening tool to detect and control illegal addition of CPFX in food products. This kit had been applied to milk detection and the recovery rates from samples spiked by CPFX were in a range of 63.02%-84.60%, with coefficients of variation of less than 12.2%.

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“…Most of them involve liquid chromatography with ultraviolet (LC-UV; Gigosos et al, 2000;Pecorelli et al, 2003;Bailac et al, 2004;Christodoulou and Samanidou, 2007), fluorescence (LC-FD;Eng et al, 1998;Hernández-Arteseros et al, 2000;Chu et al, 2002;EspinosaMansilla et al, 2005;Hassouan et al, 2007) or mass spectrometric detection (Van Vyncht et al, 2002;Toussaint et al, 2002Toussaint et al, , 2005aSchneider et al, 2005;Van Hoof et al, 2005;Hermo et al, 2006;Bailac et al, 2006;Rubies et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of quinolone antibacterials in bovine milk by liquid chromatography–mass spectrometry

Zafra-Gómez

Garballo

Ballesteros

et al. 2008

Biomedical Chromatography

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A new liquid chromatography-mass spectrometry (LC-MS) method has been developed and validated for the simultaneous determination of eight quinolone antibacterials for veterinary use in processed bovine milk samples. The quinolones studied included marbofloxacin, ciprofloxacin, danofloxacin, enrofloxacin, sarafloxacin, difloxacin, oxolinic acid and flumequine. Also, a new sample-treatment procedure was used for extraction and preconcentration of these compounds. It involved defatting by centrifugation, protein precipitation by adding a mixture of glacial acetic acid-acetonitrile and removing acetonitrile with dichloromethane; finally, the acidified aqueous layer was evaporated to dryness in a speed vac system, resuspended in the mobile phase and filtered prior to LC injection. The mobile phase was composed of a formic acid aqueous solution 0.1% (v/v) and acetonitrile, with an initial composition of water-acetonitrile 95: 5 (v/v) and using linear gradient elution. Norfloxacin was used as internal standard. The limits of quantification found (2-7 ng g(-1)) were in all cases lower than the maximum residue limits tolerated by the European Union for these compounds in milk.

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Multiresidue determination of (fluoro)quinolone antibiotics in swine kidney using liquid chromatography–tandem mass spectrometry

Cited by 78 publications

References 21 publications

Determination of quinolone residues in chicken and fish by capillary electrophoresis‐mass spectrometry

Determination of quinolone residues in chicken and fish by capillary electrophoresis‐mass spectrometry

Preparation of high-affinity rabbit monoclonal antibodies for ciprofloxacin and development of an indirect competitive ELISA for residues in milk

Simultaneous determination of quinolone antibacterials in bovine milk by liquid chromatography–mass spectrometry

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