Multiresidue analysis of neonicotinoids by solid-phase extraction technique using high-performance liquid chromatography

Abstract

For routine monitoring of pesticides, a multiresidue analysis through solid-phase extraction technique and using high-performance liquid chromatography (HPLC) in cotton seed cake (CSC) has been developed. Extraction of fortified samples was carried out with aqueous acetone under vacuum. The concentrated extract was loaded onto the solid-phase extraction units, preconditioned with acetonitrile. The extraction units were then washed with hexane and finally eluted with acetonitrile. The pesticide residues were determined using a multiresidue method by reversed-phase HPLC. The average percentage recoveries were found to range between 65.47% and 110% at spiking levels of 10 to 40 mg/kg. The method developed shows a healthy rate of recovery and can successfully be utilized for the extraction and screening of neonicotinoid residues in CSC. The detection limits for imidacloprid, acetamiprid, and thiacloprid using this method were found to be 5, 10, and 20 mg/kg, respectively.

Nicotine degradation by two novel bacterial isolates of Acinetobacter sp. TW and Sphingomonas sp. TY and their responses in the presence of neonicotinoid insecticides

“…Nicotine concentration was analyzed using methanol/0.1% triethylamine (40:60) as the mobile phase at a flow rate of 1.0 ml/min with an injection volume of 5 ll (Ruan et al 2005). Neonicotinoid insecticides were determined using an injection volume of 10 ll with acetonitrile/Milli-Q water (25:75) as the mobile phase at a flow rate of 1.2 ml/min (Obana et al 2002;Bi et al 2006;Mohan et al 2010).…”

Review on Current Analytical Methods with Chromatographic and Nonchromatographic Techniques for New Generation Insecticide Neonicotinoids

“…Table 1 shows that sample pre-treatment procedures roughly consist of (1) extraction of the target pesticide from the sample and (2) separation of the target pesticide from the extract and clean-up. For (1) extraction of neonicotinoid insecticides, shaking extraction with organic solvents such as acetone, acetonitrile, or methanol (Baskaran et al, 1997;de Erenchun et al, 1997;Mohan et al, 2010;Tokieda et al, 1997bTokieda et al, , 1998, blending extraction with a homogenizer (Agüera et al, 2004;Blasco et al, 2002aBlasco et al, , 2002bDi Muccio et al, 2006;Fernandez-Alba et al, 1996Ferrer et al, 2005;Hengel & Miller, 2008;Hernández et al, 2006;Ishii et al, 1994;Jansson et al, 2004;Kamel et al, 2010;Mateu-Sánchez et al, 2003;Obana et al, 2002Obana et al, , 2003Sannino et al, 2004;Ting et al, 2004;Tokieda et al, 1997aTokieda et al, , 1997bVenkateswarlu et al, 2007), and ultrasonic extraction (Bourgin et al, 2009;García et al, 2007;Ishii et al, 1994;Liu et al, 2005Liu et al, , 2010Mayer-Helm, 2009;Rancan et al, 2006aRancan et al, , 2006bZhang et al, 2010) are commonly used. In addition to these, Bourgin et al (2009) extracted five insecticides including imidacloprid from seeds coated with acetonitrile and Xiao et al (2011) extracted seven neonicotinoid insecticides from bovine tissues with water by accelerated solvent extraction (ASE) to give quantitative extraction efficiency.…”

“…a large amount of organic solvent is used; and an emulsion is formed at the liquid-liquid interface depending on the extraction sample that is used. For that reason, it has been increasingly replaced by clean-up mainly by SPE (Mohan et al, 2010;Obana et al, 2002;Ting et al, 2004;. used re-extraction with diatomaceous earth SPE and clean-up with GCB/NH 2 SPE in the development of simultaneous analysis by HPLC-DAD of seven neonicotinoid insecticides released on the market.…”

Aptamer-based Resonance Light Scattering for Sensitive Detection of Acetamiprid

“…Despite that it is considered to have relatively low acute and chronic mammalian toxicity, due to its frequent and extensive usage, there is probability to cause potential risk of human health. 1 At present, there are many methods used to detect acetamiprid, for instance, high-performance liquid chromatography (HPLC), [2][3][4] enzyme-linked immunosorbent assay (ELISA) 5,6 and liquid chromatography (LC) [7][8][9][10] etc. All of these methods have many advantages; however, they also encounter some disadvantages, for example: HPLC and LC suffer from complicated sample pretreatment, time consuming and inappropriate on-site operation.…”