Multipotential stromal cells in the talus and distal tibia in ankle osteoarthritis – Presence, potency and relationships to subchondral bone changes

Jones, William G.; El‐Jawhari, Jehan J.; Brockett, Claire; Koria, Lekha; Ktistakis, Ioannis; Jones, Elena

doi:10.1111/jcmm.15993

Cited by 2 publications

(1 citation statement)

References 54 publications

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“…Despite the good clinical response of patients to IO infiltrations of PRP, knowing the biological mechanisms behind this improvement is essential in advancing the development of more effective therapies. Aberrant behaviour of subchondral bone MSCs with decreased proliferation and multipotency and increased osteoblastogenesis have been previously reported in OA animal models and in human studies [13][14][15][16]. Another parameter contributing towards the progression of OA is cellular senescence.…”

Section: Introductionmentioning

confidence: 88%

Effect of Combined Intraosseous and Intraarticular Infiltrations of Autologous Platelet-Rich Plasma on Subchondral Bone Marrow Mesenchymal Stromal Cells from Patients with Hip Osteoarthritis

Ganguly

Fiz

Beitia³

et al. 2022

JCM

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Osteoarthritis (OA) is a debilitating condition that significantly impacts its patients and is closely associated with advancing age and senescence. Treatment with autologous platelet rich plasma (PRP) is a novel approach that is increasingly being researched for its effects. Subchondral bone mesenchymal stromal cells (MSCs) are key progenitors that form bone and cartilage lineages that are affected in OA. This study investigated the changes in subchondral bone MSCs before and after combined intraosseous (IO) and intraarticular (IA) PRP infiltration. Patient bone marrow aspirates were collected from 12 patients (four male, eight female) aged 40–86 years old (median 59.5). MSCs were expanded in standard media containing human serum to passage 1 and analysed for their colony-forming potential, senescence status, and gene expression. Hip dysfunction and Osteoarthritis Outcome Score (HOOS) at baseline and 6 months post second infiltration were used to assess the clinical outcomes; seven patients were considered responders and five non-responders. The number of colony-forming MSCs did not increase in the post treatment group, however, they demonstrated significantly higher colony areas (14.5% higher compared to Pre) indicative of enhanced proliferative capacity, especially in older donors (28.2% higher). Senescence assays also suggest that older patients and responders had a higher resistance to senescent cell accumulation. Responder and non-responder MSCs tended to differ in the expression of genes associated with bone formation and cartilage turnover including osteoblast markers, matrix metalloproteinases, and their inhibitors. Taken together, our data show that in hip OA patients, combined IO and IA PRP infiltrations enhanced subchondral MSC proliferative and stress-resistance capacities, particularly in older patients. Future investigation of the potential anti-ageing effect of PRP infiltrations and the use of next-generation sequencing would contribute towards better understanding of the molecular mechanisms associated with OA in MSCs.

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Section: Introductionmentioning

confidence: 88%