Multipotent precursors in the anterior and hippocampal subventricular zone display similar transcription factor signatures but their proliferation and maintenance are differentially regulated

García, Carmen Carrillo; Suh, Yongjoon; Obernier, Kirsten; Hölzl-Wenig, Gabi; Mandl, Claudia; Ciccolini, Francesca

doi:10.1016/j.mcn.2010.04.003

Cited by 21 publications

(54 citation statements)

References 41 publications

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“…2Aa). This procedure, hereafter referred to as gate setting I, was previously used for the isolation of neonatal cells2324. Since adult SEZ cells displayed high levels of autofluorescence (Fig.…”

Section: Resultsmentioning

confidence: 99%

Proliferation and cilia dynamics in neural stem cells prospectively isolated from the SEZ

Khatri

Obernier

Simeonova

et al. 2014

Sci Rep

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Neural stem cells (NSCs) generate new neurons in vivo and in vitro throughout adulthood and therefore are physiologically and clinically relevant. Unveiling the mechanisms regulating the lineage progression from NSCs to newborn neurons is critical for the transition from basic research to clinical application. However, the direct analysis of NSCs and their progeny is still elusive due to the problematic identification of the cells. We here describe the isolation of highly purified genetically unaltered NSCs and transit-amplifying precursors (TAPs) from the adult subependymal zone (SEZ). Using this approach we show that a primary cilium and high levels of epidermal growth factor receptor (EGFR) at the cell membrane characterize quiescent and cycling NSCs, respectively. However, we also observed non-ciliated quiescent NSCs and NSCs progressing into the cell cycle without up-regulating EGFR expression. Thus, the existence of NSCs displaying distinct molecular and structural conformations provides more flexibility to the regulation of quiescence and cell cycle progression.

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Section: Resultsmentioning

confidence: 99%

Proliferation and cilia dynamics in neural stem cells prospectively isolated from the SEZ

Khatri

Obernier

Simeonova

et al. 2014

Sci Rep

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“…Cells were incubated with primary PSANCAM antibody together with a fluorescently labeled secondary antibody (Molecular Probes, Darmstadt, Germany) or with prominin-PE and then stained with EGF-Alexa (20 ng/ml) as previously described [11,12]. For clonal analysis, single cells were plated by FACS-automated cell deposition in each well of 96-well plates (Nunc, Wiesbaden, Germany) in NSA proliferation medium as described before [13].…”

Section: Facs and Clonal Analysismentioning

confidence: 99%

“…It has been previously shown that clonogenic EGFR high cells include two different cell types: activated stem cells, that is, precursors expressing both high levels of EGFR and stem cell markers, and TAPs [11,12,21]. We next investigated whether diazepam affects the proliferation of stem cells or TAPs in vivo.…”

Section: Gaba a Rs Activation Increases Proliferation Of Prominin 1 Pmentioning

confidence: 99%

GABAA Receptor Signaling Induces Osmotic Swelling and Cell Cycle Activation of Neonatal Prominin+ Precursors

et al. 2011

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Signal-regulated changes in cell size affect cell division and survival and therefore are central to tissue morphogenesis and homeostasis. In this respect, GABA receptors (GABA A Rs) are of particular interest because allowing anions flow across the cell membrane modulates the osmolyte flux and the cell volume. Therefore, we have here investigated the hypothesis that GABA may regulate neural stem cell proliferation by inducing cell size changes. We found that, besides neuroblasts, also neural precursors in the neonatal murine subependymal zone sense GABA via GABA A Rs. However, unlike in neuroblasts, where it induced depolarization-mediated [Ca 21 ] i increase, GABA A Rs activation in precursors caused hyperpolarization. This resulted in osmotic swelling and increased surface expression of epidermal growth factor receptors (EGFRs). Furthermore, activation of GABA A Rs signaling in vitro in the presence of EGF modified the expression of the cell cycle regulators, phosphatase and tensin homolog and cyclin D1, increasing the pool of cycling precursors without modifying cell cycle length. A similar effect was observed on treatment with diazepam. We also demonstrate that GABA and diazepam responsive precursors represent prominin 1 stem cells. Finally, we show that as in in vitro also in in vivo a short administration of diazepam promotes EGFR expression in prominin 1 stem cells causing activation and cell cycle entry. Thus, our data indicate that endogenous GABA is a part of a regulatory mechanism of size and cell cycle entry of neonatal stem cells. Our results also have potential implications for the therapeutic practices that involve exposure to GABA A Rs modulators during neurodevelopment. STEM CELLS 2011;29:307-319 Disclosure of potential conflicts of interest is found at the end of this article.

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“…Activated NSCs and transit-amplifying precursors (TAPs) express high levels of the epidermal growth factor receptor (EGFR) at the cell surface (E high ), and they proliferate in vivo and in vitro in response to EGF (5). Because EGFR is progressively down-regulated during the differentiation of intermediate progenitors into neuroblasts (6), prominin-1 and EGFR represent useful markers for the efficient identification and isolation of activated NSCs and TAPs (7)(8)(9).…”

Section: Activation Of ␥-Aminobutyricmentioning

confidence: 99%

“…However, the distribution of AQP4 in the different precursor types of the SEZ is still unknown. To investigate this issue, we used flow cytometry to measure expression levels of EGFR and prominin-1 and purify the following cell populations from the SEZ of neonatal mice: P ϩ /E low cells consisting of ependymal cells and quiescent NSCs; P ϩ /E high cells being highly enriched in activated NSCs; P Ϫ /E high cells enriched in TAPs and pre-neuroblasts; and P Ϫ /E low cells representing mostly neuroblasts (7,9,17).…”

Section: Colocalization Of Gaba a Rs And Aqp4 In Sez Precursors-mentioning

confidence: 99%

γ-Aminobutyric A Receptor (GABAAR) Regulates Aquaporin 4 Expression in the Subependymal Zone

Schmidt-Edelkraut

Poetz

et al. 2015

Journal of Biological Chemistry

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Multipotent precursors in the anterior and hippocampal subventricular zone display similar transcription factor signatures but their proliferation and maintenance are differentially regulated

Cited by 21 publications

References 41 publications

Proliferation and cilia dynamics in neural stem cells prospectively isolated from the SEZ

Proliferation and cilia dynamics in neural stem cells prospectively isolated from the SEZ

GABAA Receptor Signaling Induces Osmotic Swelling and Cell Cycle Activation of Neonatal Prominin+ Precursors

γ-Aminobutyric A Receptor (GABAAR) Regulates Aquaporin 4 Expression in the Subependymal Zone

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